ID   THPR_GEOSE              Reviewed;         314 AA.
AC   Q45633;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   25-MAY-2022, entry version 75.
DE   RecName: Full=RNA 2',3'-cyclic phosphodiesterase {ECO:0000255|HAMAP-Rule:MF_01940};
DE            Short=RNA 2',3'-CPDase {ECO:0000255|HAMAP-Rule:MF_01940};
DE            EC=3.1.4.58 {ECO:0000255|HAMAP-Rule:MF_01940};
OS   Geobacillus stearothermophilus (Bacillus stearothermophilus).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Geobacillus.
OX   NCBI_TaxID=1422;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 7953 / DSM 5934 / JCM 9488 / NBRC 13737 / NCIB 8157 / NCTC
RC   10007 / NCA 1518;
RX   PubMed=8190087; DOI=10.1007/bf00301070;
RA   Liong E.C., Ferenci T.;
RT   "Molecular cloning of a maltose transport gene from Bacillus
RT   stearothermophilus and its expression in Escherichia coli K-12.";
RL   Mol. Gen. Genet. 243:343-352(1994).
CC   -!- FUNCTION: Hydrolyzes RNA 2',3'-cyclic phosphodiester to an RNA 2'-
CC       phosphomonoester. {ECO:0000255|HAMAP-Rule:MF_01940}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 3'-end 2',3'-cyclophospho-ribonucleotide-RNA + H2O = a 3'-
CC         end 2'-phospho-ribonucleotide-RNA + H(+); Xref=Rhea:RHEA:11828,
CC         Rhea:RHEA-COMP:10464, Rhea:RHEA-COMP:17353, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:83064, ChEBI:CHEBI:173113;
CC         EC=3.1.4.58; Evidence={ECO:0000255|HAMAP-Rule:MF_01940};
CC   -!- SIMILARITY: Belongs to the 2H phosphoesterase superfamily. ThpR family.
CC       {ECO:0000255|HAMAP-Rule:MF_01940}.
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DR   EMBL; L13418; AAA71981.1; -; Unassigned_DNA.
DR   PIR; S43916; S43916.
DR   AlphaFoldDB; Q45633; -.
DR   SMR; Q45633; -.
DR   GO; GO:0004113; F:2',3'-cyclic-nucleotide 3'-phosphodiesterase activity; IEA:InterPro.
DR   GO; GO:0008664; F:2'-5'-RNA ligase activity; IEA:InterPro.
DR   HAMAP; MF_01940; RNA_CPDase; 1.
DR   InterPro; IPR009097; Cyclic_Pdiesterase.
DR   InterPro; IPR014051; Phosphoesterase_HXTX.
DR   InterPro; IPR004175; RNA_CPDase.
DR   PANTHER; PTHR35561; PTHR35561; 1.
DR   Pfam; PF02834; LigT_PEase; 2.
DR   SUPFAM; SSF55144; SSF55144; 1.
DR   TIGRFAMs; TIGR02258; 2_5_ligase; 1.
PE   3: Inferred from homology;
KW   Hydrolase.
FT   CHAIN           1..314
FT                   /note="RNA 2',3'-cyclic phosphodiesterase"
FT                   /id="PRO_0000138967"
FT   MOTIF           43..46
FT                   /note="HXTX 1"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01940"
FT   MOTIF           129..132
FT                   /note="HXTX 2"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01940"
FT   ACT_SITE        43
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01940"
FT   ACT_SITE        129
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01940"
SQ   SEQUENCE   314 AA;  35736 MW;  B54E25FD3F72BFC4 CRC64;
     MKRSHYFIAV PLTSEAKQAI SRFSGDASSS LPFRTWVHEE DYHITLAFLG DVPPGKMAPL
     CEAMAAVAAK SAPFSLALAG LGTFGERTAP RIFWQGVKEE AALNELRRDV YEACLSLGFS
     LDRRPFAPHI TIARKWQGEA PFQPEALRSL PAASTVFSVP EIVLYRTNME KTPKYETIAA
     FPLLGAPDGR TGEGMGQLLK LRDYISRYET DVYHYVPEFI RLKQWQWEQA KARWEAERDA
     DGARREPGET WDFLLDKPSW WERLIGRWRR GPEPEMDEER SPAPSLSRAA TLDELKWQFL
     DDLFELQGMP AGRL