ID   THPR_METJA              Reviewed;         173 AA.
AC   Q58963;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   25-MAY-2022, entry version 102.
DE   RecName: Full=RNA 2',3'-cyclic phosphodiesterase {ECO:0000255|HAMAP-Rule:MF_01940};
DE            Short=RNA 2',3'-CPDase {ECO:0000255|HAMAP-Rule:MF_01940};
DE            EC=3.1.4.58 {ECO:0000255|HAMAP-Rule:MF_01940};
GN   OrderedLocusNames=MJ1568;
OS   Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM
OS   10045 / NBRC 100440) (Methanococcus jannaschii).
OC   Archaea; Euryarchaeota; Methanomada group; Methanococci; Methanococcales;
OC   Methanocaldococcaceae; Methanocaldococcus.
OX   NCBI_TaxID=243232;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440;
RX   PubMed=8688087; DOI=10.1126/science.273.5278.1058;
RA   Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G.,
RA   Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R.,
RA   Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R.,
RA   Kirkness E.F., Weinstock K.G., Merrick J.M., Glodek A., Scott J.L.,
RA   Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R.,
RA   Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D.,
RA   Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P.,
RA   Fraser C.M., Smith H.O., Woese C.R., Venter J.C.;
RT   "Complete genome sequence of the methanogenic archaeon, Methanococcus
RT   jannaschii.";
RL   Science 273:1058-1073(1996).
CC   -!- FUNCTION: Hydrolyzes RNA 2',3'-cyclic phosphodiester to an RNA 2'-
CC       phosphomonoester. {ECO:0000255|HAMAP-Rule:MF_01940}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 3'-end 2',3'-cyclophospho-ribonucleotide-RNA + H2O = a 3'-
CC         end 2'-phospho-ribonucleotide-RNA + H(+); Xref=Rhea:RHEA:11828,
CC         Rhea:RHEA-COMP:10464, Rhea:RHEA-COMP:17353, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:83064, ChEBI:CHEBI:173113;
CC         EC=3.1.4.58; Evidence={ECO:0000255|HAMAP-Rule:MF_01940};
CC   -!- SIMILARITY: Belongs to the 2H phosphoesterase superfamily. ThpR family.
CC       {ECO:0000255|HAMAP-Rule:MF_01940}.
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DR   EMBL; L77117; AAB99586.1; -; Genomic_DNA.
DR   PIR; G64495; G64495.
DR   RefSeq; WP_010871092.1; NC_000909.1.
DR   AlphaFoldDB; Q58963; -.
DR   SMR; Q58963; -.
DR   STRING; 243232.MJ_1568; -.
DR   EnsemblBacteria; AAB99586; AAB99586; MJ_1568.
DR   GeneID; 1452476; -.
DR   KEGG; mja:MJ_1568; -.
DR   eggNOG; arCOG01736; Archaea.
DR   HOGENOM; CLU_081251_3_4_2; -.
DR   InParanoid; Q58963; -.
DR   OMA; HITLRFL; -.
DR   OrthoDB; 102399at2157; -.
DR   PhylomeDB; Q58963; -.
DR   Proteomes; UP000000805; Chromosome.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0004113; F:2',3'-cyclic-nucleotide 3'-phosphodiesterase activity; IEA:InterPro.
DR   GO; GO:0008664; F:2'-5'-RNA ligase activity; IEA:InterPro.
DR   GO; GO:0034237; F:protein kinase A regulatory subunit binding; IBA:GO_Central.
DR   GO; GO:0010738; P:regulation of protein kinase A signaling; IBA:GO_Central.
DR   HAMAP; MF_01940; RNA_CPDase; 1.
DR   InterPro; IPR009097; Cyclic_Pdiesterase.
DR   InterPro; IPR014051; Phosphoesterase_HXTX.
DR   InterPro; IPR004175; RNA_CPDase.
DR   PANTHER; PTHR35561; PTHR35561; 1.
DR   Pfam; PF02834; LigT_PEase; 2.
DR   SUPFAM; SSF55144; SSF55144; 1.
DR   TIGRFAMs; TIGR02258; 2_5_ligase; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Reference proteome.
FT   CHAIN           1..173
FT                   /note="RNA 2',3'-cyclic phosphodiesterase"
FT                   /id="PRO_0000138963"
FT   MOTIF           38..41
FT                   /note="HXTX 1"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01940"
FT   MOTIF           118..121
FT                   /note="HXTX 2"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01940"
FT   ACT_SITE        38
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01940"
FT   ACT_SITE        118
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01940"
SQ   SEQUENCE   173 AA;  20545 MW;  34F03BCE0159E5B1 CRC64;
     MRLFLAIDIP ENIKEEIAKF QEQFKMKGIK LVEKENLHIT VKFLGEVDEE KLKEILNLDL
     SIQPIKIKLK YIGTFPNSNY IRVIWIGAYN NNLVEIFKEI DEKLSNLGFK KEREYVPHLT
     IGRVKFIDNK KKLKDRIEKY KDVDFGEFEA KHIKLYKSTL TPNGPIYEVI KEW