THPR_THET8
ID THPR_THET8 Reviewed; 198 AA.
AC Q5SHB1; Q84CU4;
DT 04-FEB-2015, integrated into UniProtKB/Swiss-Prot.
DT 21-DEC-2004, sequence version 1.
DT 25-MAY-2022, entry version 84.
DE RecName: Full=RNA 2',3'-cyclic phosphodiesterase {ECO:0000255|HAMAP-Rule:MF_01940};
DE Short=RNA 2',3'-CPDase {ECO:0000255|HAMAP-Rule:MF_01940};
DE EC=3.1.4.58 {ECO:0000255|HAMAP-Rule:MF_01940};
GN OrderedLocusNames=TTHA1819 {ECO:0000312|EMBL:BAD71642.1};
OS Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8).
OC Bacteria; Deinococcus-Thermus; Deinococci; Thermales; Thermaceae; Thermus.
OX NCBI_TaxID=300852;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], X-RAY CRYSTALLOGRAPHY (2.50 ANGSTROMS),
RP AND ACTIVE SITES.
RC STRAIN=ATCC 27634 / DSM 579 / HB8;
RX PubMed=12798681; DOI=10.1016/s0022-2836(03)00448-0;
RA Kato M., Shirouzu M., Terada T., Yamaguchi H., Murayama K., Sakai H.,
RA Kuramitsu S., Yokoyama S.;
RT "Crystal structure of the 2'-5' RNA ligase from Thermus thermophilus HB8.";
RL J. Mol. Biol. 329:903-911(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 27634 / DSM 579 / HB8;
RA Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T.,
RA Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.;
RT "Complete genome sequence of Thermus thermophilus HB8.";
RL Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Hydrolyzes RNA 2',3'-cyclic phosphodiester to an RNA 2'-
CC phosphomonoester. {ECO:0000255|HAMAP-Rule:MF_01940}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 3'-end 2',3'-cyclophospho-ribonucleotide-RNA + H2O = a 3'-
CC end 2'-phospho-ribonucleotide-RNA + H(+); Xref=Rhea:RHEA:11828,
CC Rhea:RHEA-COMP:10464, Rhea:RHEA-COMP:17353, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:83064, ChEBI:CHEBI:173113;
CC EC=3.1.4.58; Evidence={ECO:0000255|HAMAP-Rule:MF_01940};
CC -!- SIMILARITY: Belongs to the 2H phosphoesterase superfamily. ThpR family.
CC {ECO:0000255|HAMAP-Rule:MF_01940}.
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DR EMBL; AB107665; BAC67697.1; -; Genomic_DNA.
DR EMBL; AP008226; BAD71642.1; -; Genomic_DNA.
DR RefSeq; WP_011173843.1; NC_006461.1.
DR RefSeq; YP_145085.1; NC_006461.1.
DR PDB; 1IUH; X-ray; 2.50 A; A=1-198.
DR PDBsum; 1IUH; -.
DR AlphaFoldDB; Q5SHB1; -.
DR SMR; Q5SHB1; -.
DR STRING; 300852.55773201; -.
DR EnsemblBacteria; BAD71642; BAD71642; BAD71642.
DR GeneID; 3168502; -.
DR KEGG; ttj:TTHA1819; -.
DR PATRIC; fig|300852.9.peg.1790; -.
DR eggNOG; COG1514; Bacteria.
DR HOGENOM; CLU_081251_0_1_0; -.
DR OMA; HITLRFL; -.
DR PhylomeDB; Q5SHB1; -.
DR Proteomes; UP000000532; Chromosome.
DR GO; GO:0004113; F:2',3'-cyclic-nucleotide 3'-phosphodiesterase activity; IEA:InterPro.
DR GO; GO:0008664; F:2'-5'-RNA ligase activity; IEA:InterPro.
DR HAMAP; MF_01940; RNA_CPDase; 1.
DR InterPro; IPR009097; Cyclic_Pdiesterase.
DR InterPro; IPR014051; Phosphoesterase_HXTX.
DR InterPro; IPR004175; RNA_CPDase.
DR PANTHER; PTHR35561; PTHR35561; 1.
DR Pfam; PF02834; LigT_PEase; 2.
DR SUPFAM; SSF55144; SSF55144; 1.
DR TIGRFAMs; TIGR02258; 2_5_ligase; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Hydrolase; Reference proteome.
FT CHAIN 1..198
FT /note="RNA 2',3'-cyclic phosphodiesterase"
FT /id="PRO_0000431802"
FT MOTIF 39..42
FT /note="HXTX 1"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01940,
FT ECO:0000305|PubMed:12798681"
FT MOTIF 130..133
FT /note="HXTX 2"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01940,
FT ECO:0000305|PubMed:12798681"
FT ACT_SITE 39
FT /note="Proton donor"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01940,
FT ECO:0000305|PubMed:12798681"
FT ACT_SITE 130
FT /note="Proton acceptor"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01940,
FT ECO:0000305|PubMed:12798681"
FT STRAND 2..8
FT /evidence="ECO:0007829|PDB:1IUH"
FT HELIX 11..21
FT /evidence="ECO:0007829|PDB:1IUH"
FT HELIX 22..24
FT /evidence="ECO:0007829|PDB:1IUH"
FT STRAND 30..32
FT /evidence="ECO:0007829|PDB:1IUH"
FT HELIX 35..37
FT /evidence="ECO:0007829|PDB:1IUH"
FT STRAND 39..47
FT /evidence="ECO:0007829|PDB:1IUH"
FT HELIX 50..52
FT /evidence="ECO:0007829|PDB:1IUH"
FT HELIX 53..66
FT /evidence="ECO:0007829|PDB:1IUH"
FT STRAND 70..85
FT /evidence="ECO:0007829|PDB:1IUH"
FT STRAND 87..94
FT /evidence="ECO:0007829|PDB:1IUH"
FT HELIX 96..113
FT /evidence="ECO:0007829|PDB:1IUH"
FT HELIX 114..119
FT /evidence="ECO:0007829|PDB:1IUH"
FT TURN 121..124
FT /evidence="ECO:0007829|PDB:1IUH"
FT STRAND 130..138
FT /evidence="ECO:0007829|PDB:1IUH"
FT STRAND 150..154
FT /evidence="ECO:0007829|PDB:1IUH"
FT STRAND 156..164
FT /evidence="ECO:0007829|PDB:1IUH"
FT STRAND 166..178
FT /evidence="ECO:0007829|PDB:1IUH"
SQ SEQUENCE 198 AA; 22411 MW; D85B66890413350E CRC64;
MRLFYAVFLP EEVRAALVEA QTKVRPFRGW KPVPPHQLHL TLLFLGERPE EELPDYLALG
HRLARLEAPF RARLRGTGYF PNEGTPRVWF AKAEAEGFLR LAEGLRAGVE ELLGEEAVRI
PGWDKPFKPH ITLARRKAPA PRVPPVLFGL EWPVEGFALV RSELKPKGPV YTVLEKFSLR
GEHGREQAQG PGERPEGD
