ID   THR1_COLOR              Reviewed;         282 AA.
AC   P87025; A0A484G104; N4VTL6;
DT   16-JAN-2004, integrated into UniProtKB/Swiss-Prot.
DT   18-SEP-2013, sequence version 4.
DT   03-AUG-2022, entry version 94.
DE   RecName: Full=Trihydroxynaphthalene reductase;
DE            EC=1.1.1.-;
DE   AltName: Full=T3HN reductase;
GN   Name=THR1; ORFNames=Cob_04570, Cob_v003479;
OS   Colletotrichum orbiculare (strain 104-T / ATCC 96160 / CBS 514.97 / LARS
OS   414 / MAFF 240422) (Cucumber anthracnose fungus) (Colletotrichum
OS   lagenarium).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Hypocreomycetidae; Glomerellales; Glomerellaceae; Colletotrichum;
OC   Colletotrichum orbiculare species complex.
OX   NCBI_TaxID=1213857;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=104-T / ATCC 96160 / CBS 514.97 / LARS 414 / MAFF 240422;
RX   PubMed=8672814; DOI=10.1094/mpmi-9-0323;
RA   Perpetua N.S., Kubo Y., Yasuda N., Takano Y., Furusawa I.;
RT   "Cloning and characterization of a melanin biosynthetic THR1 reductase gene
RT   essential for appressorial penetration of Colletotrichum lagenarium.";
RL   Mol. Plant Microbe Interact. 9:323-329(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=104-T / ATCC 96160 / CBS 514.97 / LARS 414 / MAFF 240422;
RX   PubMed=23252678; DOI=10.1111/nph.12085;
RA   Gan P., Ikeda K., Irieda H., Narusaka M., O'Connell R.J., Narusaka Y.,
RA   Takano Y., Kubo Y., Shirasu K.;
RT   "Comparative genomic and transcriptomic analyses reveal the hemibiotrophic
RT   stage shift of Colletotrichum fungi.";
RL   New Phytol. 197:1236-1249(2013).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=104-T / ATCC 96160 / CBS 514.97 / LARS 414 / MAFF 240422;
RX   PubMed=30893003; DOI=10.1094/mpmi-12-18-0352-a;
RA   Gan P., Tsushima A., Narusaka M., Narusaka Y., Takano Y., Kubo Y.,
RA   Shirasu K.;
RT   "Genome sequence resources for four phytopathogenic fungi from the
RT   Colletotrichum orbiculare species complex.";
RL   Mol. Plant Microbe Interact. 32:1088-1090(2019).
CC   -!- FUNCTION: Catalyzes the NADPH-dependent reduction of 1,3,8-
CC       trihydroxynaphthalene (T3HN) into (-)-vermelone. Essential for
CC       appressorial penetration of colletotrichum lagenarium.
CC   -!- PATHWAY: Pigment biosynthesis; melanin biosynthesis.
CC   -!- SUBUNIT: Homotetramer.
CC   -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR)
CC       family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAA18962.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; D83988; BAA18962.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; KB725704; ENH87277.1; -; Genomic_DNA.
DR   EMBL; AMCV02000005; TDZ23821.1; -; Genomic_DNA.
DR   AlphaFoldDB; P87025; -.
DR   SMR; P87025; -.
DR   STRING; 1213857.P87025; -.
DR   EnsemblFungi; ENH87277; ENH87277; Cob_04570.
DR   eggNOG; KOG0725; Eukaryota.
DR   HOGENOM; CLU_010194_1_3_1; -.
DR   OrthoDB; 913128at2759; -.
DR   UniPathway; UPA00785; -.
DR   PHI-base; PHI:59; -.
DR   Proteomes; UP000014480; Unassembled WGS sequence.
DR   GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR   GO; GO:0042438; P:melanin biosynthetic process; IEA:UniProtKB-UniPathway.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR002347; SDR_fam.
DR   PRINTS; PR00081; GDHRDH.
DR   PRINTS; PR00080; SDRFAMILY.
DR   SUPFAM; SSF51735; SSF51735; 1.
PE   3: Inferred from homology;
KW   Melanin biosynthesis; NADP; Oxidoreductase; Reference proteome.
FT   CHAIN           1..282
FT                   /note="Trihydroxynaphthalene reductase"
FT                   /id="PRO_0000054783"
FT   ACT_SITE        178
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250"
FT   BINDING         33..57
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250"
FT   BINDING         164
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   282 AA;  29849 MW;  69BDAB12F6DA9B23 CRC64;
     MPGVTSQSAG SKYDAIPGPL GLASASLMGK VALVTGAGRG IGREMAMELG RRGAKVIVNY
     ANSAETAEEV VQAIKKSGSD AASIKANVSD VDQIVKMFGE AKQIWGRLDI VCSNSGVVSF
     GHVKDVTPEE FDRVFAINTR GQFFVAREAY KHLEVGGRLI LMGSITGQAK GVPKHAVYSG
     SKGTIETFVR CMAIDFGDKK ITVNAVAPGG IKTDMYRDVC REYIPNGGEL DDEGVDEFAA
     GWSPMHRVGL PIDIARVVCF LASQDGEWIN GKVLGIDGAA CM