BRWD3_MOUSE
ID BRWD3_MOUSE Reviewed; 1799 AA.
AC A2AHJ4; Q3TQR7; Q3UTB6; Q640P3; Q8C7D5;
DT 17-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT 20-FEB-2007, sequence version 1.
DT 03-AUG-2022, entry version 117.
DE RecName: Full=Bromodomain and WD repeat-containing protein 3;
GN Name=Brwd3; Synonyms=Gm596;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3), AND NUCLEOTIDE
RP SEQUENCE [LARGE SCALE MRNA] OF 1595-1799 (ISOFORM 1).
RC STRAIN=C57BL/6J; TISSUE=Egg, and Pancreas;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1390-1799 (ISOFORM 1).
RC STRAIN=C57BL/6J; TISSUE=Eye;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-884; SER-885; SER-1576 AND
RP SER-1760, AND IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Kidney, Lung, Pancreas, Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Plays a role in the regulation of cell morphology and
CC cytoskeletal organization. Required in the control of cell shape (By
CC similarity). {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=A2AHJ4-1; Sequence=Displayed;
CC Name=2;
CC IsoId=A2AHJ4-2; Sequence=VSP_024310, VSP_024311;
CC Name=3;
CC IsoId=A2AHJ4-3; Sequence=VSP_024308, VSP_024309;
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DR EMBL; AK050539; BAC34314.1; -; mRNA.
DR EMBL; AK139563; BAE24064.1; -; mRNA.
DR EMBL; AK163354; BAE37315.1; -; mRNA.
DR EMBL; AL731764; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BX000698; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC082562; AAH82562.1; -; mRNA.
DR CCDS; CCDS41100.1; -. [A2AHJ4-1]
DR RefSeq; NP_001074946.1; NM_001081477.1. [A2AHJ4-1]
DR AlphaFoldDB; A2AHJ4; -.
DR SMR; A2AHJ4; -.
DR BioGRID; 238286; 64.
DR IntAct; A2AHJ4; 54.
DR STRING; 10090.ENSMUSP00000123588; -.
DR iPTMnet; A2AHJ4; -.
DR PhosphoSitePlus; A2AHJ4; -.
DR EPD; A2AHJ4; -.
DR MaxQB; A2AHJ4; -.
DR PaxDb; A2AHJ4; -.
DR PeptideAtlas; A2AHJ4; -.
DR PRIDE; A2AHJ4; -.
DR ProteomicsDB; 265242; -. [A2AHJ4-1]
DR ProteomicsDB; 265243; -. [A2AHJ4-2]
DR ProteomicsDB; 265244; -. [A2AHJ4-3]
DR Antibodypedia; 537; 52 antibodies from 16 providers.
DR Ensembl; ENSMUST00000101283; ENSMUSP00000098841; ENSMUSG00000063663. [A2AHJ4-3]
DR Ensembl; ENSMUST00000150434; ENSMUSP00000123588; ENSMUSG00000063663. [A2AHJ4-1]
DR GeneID; 382236; -.
DR KEGG; mmu:382236; -.
DR UCSC; uc009ucm.1; mouse. [A2AHJ4-1]
DR UCSC; uc009ucn.1; mouse. [A2AHJ4-2]
DR CTD; 254065; -.
DR MGI; MGI:3029414; Brwd3.
DR VEuPathDB; HostDB:ENSMUSG00000063663; -.
DR eggNOG; KOG0644; Eukaryota.
DR GeneTree; ENSGT00950000183107; -.
DR HOGENOM; CLU_001108_0_0_1; -.
DR InParanoid; A2AHJ4; -.
DR OMA; TVVTSWK; -.
DR OrthoDB; 240778at2759; -.
DR PhylomeDB; A2AHJ4; -.
DR TreeFam; TF324197; -.
DR BioGRID-ORCS; 382236; 2 hits in 77 CRISPR screens.
DR ChiTaRS; Brwd3; mouse.
DR PRO; PR:A2AHJ4; -.
DR Proteomes; UP000000589; Chromosome X.
DR RNAct; A2AHJ4; protein.
DR Bgee; ENSMUSG00000063663; Expressed in embryonic post-anal tail and 212 other tissues.
DR ExpressionAtlas; A2AHJ4; baseline and differential.
DR Genevisible; A2AHJ4; MM.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0007010; P:cytoskeleton organization; ISS:UniProtKB.
DR GO; GO:0008360; P:regulation of cell shape; ISS:UniProtKB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR Gene3D; 1.20.920.10; -; 2.
DR Gene3D; 2.130.10.10; -; 2.
DR InterPro; IPR001487; Bromodomain.
DR InterPro; IPR036427; Bromodomain-like_sf.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR019775; WD40_repeat_CS.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR Pfam; PF00439; Bromodomain; 2.
DR Pfam; PF00400; WD40; 5.
DR PRINTS; PR00503; BROMODOMAIN.
DR SMART; SM00297; BROMO; 2.
DR SMART; SM00320; WD40; 8.
DR SUPFAM; SSF47370; SSF47370; 2.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS50014; BROMODOMAIN_2; 2.
DR PROSITE; PS00678; WD_REPEATS_1; 2.
DR PROSITE; PS50082; WD_REPEATS_2; 5.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Bromodomain; Phosphoprotein; Reference proteome;
KW Repeat; WD repeat.
FT CHAIN 1..1799
FT /note="Bromodomain and WD repeat-containing protein 3"
FT /id="PRO_0000283090"
FT REPEAT 170..209
FT /note="WD 1"
FT REPEAT 213..251
FT /note="WD 2"
FT REPEAT 255..297
FT /note="WD 3"
FT REPEAT 307..347
FT /note="WD 4"
FT REPEAT 353..393
FT /note="WD 5"
FT REPEAT 400..452
FT /note="WD 6"
FT REPEAT 456..495
FT /note="WD 7"
FT REPEAT 502..542
FT /note="WD 8"
FT DOMAIN 1156..1226
FT /note="Bromo 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00035"
FT DOMAIN 1315..1409
FT /note="Bromo 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00035"
FT REGION 766..912
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1258..1291
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1321..1367
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1435..1482
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1517..1723
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 799..818
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 841..859
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 869..885
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1329..1351
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1450..1465
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1518..1532
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1533..1547
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1563..1598
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1599..1623
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1624..1645
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1646..1660
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1677..1691
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 693
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q6RI45"
FT MOD_RES 884
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 885
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 1574
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q6RI45"
FT MOD_RES 1576
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 1760
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT VAR_SEQ 144..181
FT /note="VNITSARQLTGCSRFSHVFPSSAYQHIKMHKRILGHLS -> GFFFVALTVL
FT ELYVDQAGLCLSSAGIKVVCHMTSYITF (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_024308"
FT VAR_SEQ 182..1799
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_024309"
FT VAR_SEQ 272..283
FT /note="FCPSTKGTTRYL -> VRIMISSLPG (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_024310"
FT VAR_SEQ 284..1799
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_024311"
SQ SEQUENCE 1799 AA; 202942 MW; 57E01C1ECBDD2D31 CRC64;
MAAAPTQIEA ELYYLIARFL QSGPCNKSAQ VLVQELEEHQ LIPRRLDWEG KEHRRSFEDL
VAANAHIPPD YLLKICERIG PLLDKEIPQS VPGVQTLLGV GRQSLLRDAK DCKSTLWNGS
AFAALHRGRP PELPVNYVKP PNVVNITSAR QLTGCSRFSH VFPSSAYQHI KMHKRILGHL
SSVYCVAFDR SGRRIFTGSD DCLVKIWATD DGRLLATLRG HSAEISDMAV NYENTLIAAG
SCDKVVRVWC LRTCAPVAVL QGHSASITSI QFCPSTKGTT RYLTSTGADG TICFWQWHVK
TMKFRDRPVK FTERSRPGVQ ISCSSFSSGG MFITTGSTDH VIRIYYLGSE IPEKIAELES
HTDKVVAVQF CNNGDSLRFV SGSRDGTARI WQYQQQEWKS IVLDMATKMS GNNLTSAEDK
VTKLKVTMVA WDRYDTTVIT AVNNFLLKVW NSVTGQLLHT LSGHDDEVFV LEAHPFDQRI
ILSAGHDGNI FIWDLDRGTK IRNYFNMIEG QGHGAVFDCK FSPDGNHFAC TDSHGHLLLF
GFGCSKYYEK IPDQMFFHTD YRPLIRDANN YVLDEQTQQA PHLMPPPFLV DVDGNPHPTK
FQRLVPGREN CKDEQLIPQL GYVANGDGEV VEQVIGQQTN DQEESILDGI IRELQREQDL
RLINEGDVPH FPINRSYSVN GALSSPNMDI PSSPNIGLRR SGQIEGVRQM HNNAPRSQMA
TERDLMAWSR RVVVNELNSG VSRVQEECRN AKGDLEVSLY TVEKKKKPSY PIQRNDYQPS
CGRSLRRTQR KRQHTYLTRS NIEHNSQASS QTSGVQEDSD SSSEEDETVG TSDASVEDPV
VEWQSESSSS DSSSEYSDWI ADAGINLQPP KRQTRQATQK IYSSSEDENL KLEDRQKKPK
QTKKKKGGLV SMAGEPNEEW LAPQWILDTI PRRSPFVPQM GDELIYFRQG HEAYVRAVRK
SKIYSVNLQK QPWNKMDLRE QEFVKIVGIK YEIGPPTLCC LKLAFLDPIS GKMTGESFSI
KYHDMPDVID FLVLHQFYNE AKERNWQIGD RFRSIIDDAW WFGTVESQQP FQPEYPDSSF
QCYSVHWDNN EREKMSPWDM EPIPDGTAFP DEVGAGIPVS QEELTALLYK PQEGEWGAHS
RDEECERVIQ GINNLLSLDF ASPFAVPVDL SAYPLYCTVV AYPTDLNTIK QRLENRFYRR
ISALMWEVRY IEHNARTFNE PDSPIVKAAK IVTDVLLRFI GDQSCSDILD TYNKIKAEDP
DSSDLEEDSE MVDLDSDGPG TSSGRRAKCR GRRQSLKCNP DAWKKQCEEL LSLIYEREDS
EPFRQPADPQ SYPVQQQQEG ESSQSVPPDR QDPSLSEDYQ DGIDTPMDFS TVKETLESGN
YDSPLEFYKD VRQIFSNSKA YTSNKKSRIY SMTLRLSALF ENHIKNIISD YKSAIQSQKR
RRPRYRKRLR SSSSSLSSSR APSPKGKQKQ MKLQPKNDQN TSVAYARTSS PFSSPVSDAA
EGVSLYLLDD EGDGPFSPSS FSGYSRSGNS HDPGKAKSFR NRVLPAKQDH SLDGPLTNVD
GREPRTGAKR KLLSASEEDE SMGGEEKEMK ETKEQVHLSS SESGELGSSL SSESTSGSDS
DSESTSRTDQ DYVDGDHDYS KFIQTRPKRK LRKQHTNGKR NWKTRGTGGR GRWGRWGRWS
RGGRGRGGRG RGGRGRGGGG GRGRGRGRGG RGASRGSSRA KRARVADDEF DTMFSGRFSR
LPRIKTRNQG RRTVLYNDDS DNDNFVSTED PLNLGTSRSG RVRKMTEKAR VSHLMGWNY
