THR_DROME
ID THR_DROME Reviewed; 1379 AA.
AC P42286; Q8MQL8; Q94525; Q9V894;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 19-JUL-2005, sequence version 2.
DT 03-AUG-2022, entry version 127.
DE RecName: Full=Protein three rows;
GN Name=thr; ORFNames=CG5785;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND DEVELOPMENTAL STAGE.
RC STRAIN=Oregon-R;
RX PubMed=8270646; DOI=10.1242/jcs.106.1.87;
RA Philp A.V., Axton J.M., Saunders R.D.C., Glover D.M.;
RT "Mutations in the Drosophila melanogaster gene three rows permit aspects of
RT mitosis to continue in the absence of chromatid segregation.";
RL J. Cell Sci. 106:87-98(1993).
RN [2]
RP ERRATUM OF PUBMED:8270646.
RA Philp A.V., Axton J.M., Saunders R.D., Glover D.M.;
RL J. Cell Sci. 107:1102-1102(1994).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [6]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 81-1209.
RC STRAIN=Canton-S, and Oregon-R;
RX PubMed=8305737; DOI=10.1091/mbc.4.11.1161;
RA D'Andrea R.J., Stratmann R., Lehner C.F., John U.P., Saint R.;
RT "The three rows gene of Drosophila melanogaster encodes a novel protein
RT that is required for chromosome disjunction during mitosis.";
RL Mol. Biol. Cell 4:1161-1174(1993).
RN [7]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=9927461; DOI=10.1093/genetics/151.2.685;
RA Liu X., Kiss I., Lengyel J.A.;
RT "Identification of genes controlling malpighian tubule and other epithelial
RT morphogenesis in Drosophila melanogaster.";
RL Genetics 151:685-695(1999).
RN [8]
RP FUNCTION, AND INTERACTION WITH PIM AND SSE.
RX PubMed=11581162; DOI=10.1101/gad.207301;
RA Jager H., Herzig A., Lehner C.F., Heidmann S.;
RT "Drosophila separase is required for sister chromatid separation and binds
RT to PIM and THR.";
RL Genes Dev. 15:2572-2584(2001).
RN [9]
RP FUNCTION, SUBCELLULAR LOCATION, AND MUTAGENESIS OF ARG-1035.
RX PubMed=12231632; DOI=10.1101/gad.242202;
RA Herzig A., Lehner C.F., Heidmann S.;
RT "Proteolytic cleavage of the THR subunit during anaphase limits Drosophila
RT separase function.";
RL Genes Dev. 16:2443-2454(2002).
CC -!- FUNCTION: Required specifically for chromosome disjunction during all
CC mitoses; maternally provided protein is sufficient until mitosis 14
CC then zygotic protein is required. Involved in formation and/or
CC maintenance of epithelial structures: bud extension during Malpighian
CC tubule development, and foregut and hindgut morphogenesis.
CC {ECO:0000269|PubMed:11581162, ECO:0000269|PubMed:12231632,
CC ECO:0000269|PubMed:8270646, ECO:0000269|PubMed:9927461}.
CC -!- SUBUNIT: Interacts with pim and Sse. Cleavage of thr contributes to
CC inactivation of Sse. {ECO:0000269|PubMed:11581162}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:12231632}.
CC Note=Cytoplasmic during interphase and distributed throughout the cell
CC during early mitosis.
CC -!- TISSUE SPECIFICITY: During embryogenesis, expressed in Malpighian
CC tubule buds, and epithelia of foregut and hindgut.
CC {ECO:0000269|PubMed:9927461}.
CC -!- DEVELOPMENTAL STAGE: Expressed both maternally and zygotically.
CC {ECO:0000269|PubMed:8270646}.
CC -!- PTM: Proteolytically cleaved after the metaphase-to-anaphase
CC transition, C-terminal cleavage product is degraded. Cleavage can only
CC proceed within complexes that contain active Sse.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAB29824.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=AAB60210.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=AAM75095.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=CAA53148.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=CAA53148.1; Type=Miscellaneous discrepancy; Note=Intron retention.; Evidence={ECO:0000305};
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DR EMBL; X75374; CAA53148.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AE013599; AAF57778.3; -; Genomic_DNA.
DR EMBL; AY128502; AAM75095.1; ALT_FRAME; mRNA.
DR EMBL; U03276; AAB60210.1; ALT_FRAME; Unassigned_DNA.
DR EMBL; S69585; AAB29824.1; ALT_FRAME; Genomic_DNA.
DR PIR; A49440; A49440.
DR RefSeq; NP_001286552.1; NM_001299623.1.
DR RefSeq; NP_725731.2; NM_166257.2.
DR AlphaFoldDB; P42286; -.
DR BioGRID; 62727; 7.
DR IntAct; P42286; 1.
DR STRING; 7227.FBpp0086004; -.
DR PaxDb; P42286; -.
DR PRIDE; P42286; -.
DR GeneID; 37042; -.
DR KEGG; dme:Dmel_CG5785; -.
DR CTD; 37042; -.
DR FlyBase; FBgn0003701; thr.
DR VEuPathDB; VectorBase:FBgn0003701; -.
DR eggNOG; ENOG502T8T7; Eukaryota.
DR HOGENOM; CLU_255337_0_0_1; -.
DR InParanoid; P42286; -.
DR BioGRID-ORCS; 37042; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 37042; -.
DR PRO; PR:P42286; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR ExpressionAtlas; P42286; baseline and differential.
DR Genevisible; P42286; DM.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0007502; P:digestive tract mesoderm development; IMP:FlyBase.
DR GO; GO:0007440; P:foregut morphogenesis; IMP:FlyBase.
DR GO; GO:0008406; P:gonad development; IMP:FlyBase.
DR GO; GO:0008258; P:head involution; IMP:FlyBase.
DR GO; GO:0007442; P:hindgut morphogenesis; IMP:FlyBase.
DR GO; GO:0007443; P:Malpighian tubule morphogenesis; IMP:FlyBase.
DR GO; GO:0000070; P:mitotic sister chromatid segregation; TAS:FlyBase.
DR GO; GO:0051306; P:mitotic sister chromatid separation; IMP:FlyBase.
DR GO; GO:0002009; P:morphogenesis of an epithelium; IMP:FlyBase.
DR GO; GO:0007424; P:open tracheal system development; IMP:FlyBase.
PE 1: Evidence at protein level;
KW Cell cycle; Cell division; Cytoplasm; Developmental protein; Mitosis;
KW Reference proteome.
FT CHAIN 1..1379
FT /note="Protein three rows"
FT /id="PRO_0000072526"
FT REGION 1031..1037
FT /note="Separase cleavage-site"
FT REGION 1206..1231
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1248..1309
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1328..1379
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1215..1229
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1248..1270
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1285..1307
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1328..1370
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VARIANT 392
FT /note="S -> T (in strain: Berkeley and Oregon-R)"
FT VARIANT 427
FT /note="R -> K (in strain: Berkeley and Oregon-R)"
FT VARIANT 428
FT /note="A -> T (in strain: Berkeley and Oregon-R)"
FT VARIANT 927
FT /note="L -> P (in strain: Canton-S)"
FT MUTAGEN 1035
FT /note="R->D: Abolishes proteolytic cleavage."
FT /evidence="ECO:0000269|PubMed:12231632"
FT CONFLICT 18
FT /note="K -> N (in Ref. 1)"
FT /evidence="ECO:0000305"
FT CONFLICT 216
FT /note="A -> P (in Ref. 1 and 5)"
FT /evidence="ECO:0000305"
FT CONFLICT 296
FT /note="G -> A (in Ref. 5; AAM75095)"
FT /evidence="ECO:0000305"
FT CONFLICT 315
FT /note="H -> N (in Ref. 5; AAM75095)"
FT /evidence="ECO:0000305"
FT CONFLICT 319
FT /note="S -> L (in Ref. 5; AAM75095)"
FT /evidence="ECO:0000305"
FT CONFLICT 365
FT /note="E -> D (in Ref. 3; AAF57778)"
FT /evidence="ECO:0000305"
FT CONFLICT 431
FT /note="S -> N (in Ref. 5; AAM75095)"
FT /evidence="ECO:0000305"
FT CONFLICT 624
FT /note="K -> N (in Ref. 1 and 5)"
FT /evidence="ECO:0000305"
FT CONFLICT 630
FT /note="K -> M (in Ref. 5; AAM75095)"
FT /evidence="ECO:0000305"
FT CONFLICT 633
FT /note="I -> M (in Ref. 5; AAM75095)"
FT /evidence="ECO:0000305"
FT CONFLICT 718
FT /note="D -> S (in Ref. 5)"
FT /evidence="ECO:0000305"
FT CONFLICT 756
FT /note="E -> Q (in Ref. 5)"
FT /evidence="ECO:0000305"
FT CONFLICT 915
FT /note="N -> S (in Ref. 5)"
FT /evidence="ECO:0000305"
FT CONFLICT 938
FT /note="C -> S (in Ref. 6; AAB60210/AAB29824)"
FT /evidence="ECO:0000305"
FT CONFLICT 1019
FT /note="S -> R (in Ref. 3; AAF57778)"
FT /evidence="ECO:0000305"
FT CONFLICT 1105
FT /note="E -> D (in Ref. 3; AAF57778)"
FT /evidence="ECO:0000305"
FT CONFLICT 1113
FT /note="N -> S (in Ref. 3; AAF57778)"
FT /evidence="ECO:0000305"
FT CONFLICT 1214
FT /note="T -> I (in Ref. 3; AAF57778)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1379 AA; 157511 MW; 5716E099FA05D5DB CRC64;
MSTDIATQLK GSRSDVEKVR KTVEAKFREL SGDGLPLRYE VNVLRHICLA LKDNLHQNSD
LYCDIMGIML PRVVPCEEKP SLWEAHLSSL RYIHHGLFHQ RSIEACQKLY NLIRQQPCRL
QEESDYKIYL DIHLTHFNGF HVLLQKQKLP LEATSQLCYA LESLGDLFAA MTQRQISLCA
TLLVQLNESL FGKRSRSFFK SLSFLPSESL AKMFNALLML LASSTSSNLA NLFPECLSLT
LALVQIDMFS PQSNQQMSLQ LLRMSKELFR QESNLCYALQ LMYYYIKLIF VREPTGDFKR
TYIDLSSKFQ HFFEHKVASH AKEQWLADFL VAIQLLQVLI HQSNSKLQSP FQIFWQQFDG
ESSPEIYTAH FQLLQTCASL AVNITRSPLG CSCSHEACKS VRRHCILAYG LCALDAYINW
KPAAEQRANV SPHKPLLGVV KYSMDVAKTM KCLGPTSVEI IKLVRQLTYV ADQVTCPEQM
SVLLPLLEPL QKLRPLVADQ DMSSLLRRLF KASSHCGDSN IACRIQASYL ASITNPARLR
SQVCLYYHNL GKKGTEIKRC VYEWHESTPL PFPLTPDQKK QLYDTDFFAL LHYLRSPSTA
HMESLIRCRT SDYHLVLLAR QMRKDDSISK KCIEVHDKLR QQRSLSRMDN LCLGHASVGL
LLDALEAQKT KVSTKEITEN MFEELLLSKN LWQMNIQREQ RLVNYASEAI SAFSNFFDRA
DQEPLSANET SIDWEALIDD AIATANALSS MGYQSEEDDA WLLLLRMGRL LEDRFTYLRA
LNHFLSQNEV SSRLNLKLGE EVEVAEELLD DLWPQLKNGK FFKRQQTTVM LCFCHLASYY
ARMECYSHAQ LLLLHVEQLR EEFPERQGKS DIVLLTLQTV RFRIGYQQRK PTNCRLPTPL
RQLDILLDNV RSFCNLSSLD GGSLQLLLST LVRESTECSA NRLSERLSFS NIALHLVLQS
GLALRAIEVF LAWLWTNLQM ESFDKAQSKL RLIEHCLGIK QLNPTSRPEK EAIKDVAISD
LASNMHLLQL VEPIRKQQLL NMASPNLLKM RPHSPNPQLD LDRYITLDVA PANLRENSQL
QCLYFVTGCL HARLRFLQRN SEQLEEFYGR AHNWMQEKPP MSSALYPMLH AQQLYHLNYL
RFARKHVEAI STAQLGLKMR SRAVDINFEY NFLAQLKTAQ LELKPVGQDK PQVKILRRAL
VFNHSPEDKK RTATGSVSAV KNTASKVKQS AKKAPRFRIY EELELRPPSA TSCSSSGGSG
TENTPPSDHV DLNACQAIEI SDDDDSPLVS TKKTQPKSRE KAKPKATSKA CKVLTLDNSL
EIVETPTITT STRSTRARLR QPVETPKTAT LSSKRTRRQV LEAQAPETES ISTRTRHRH
