THS2_VITVI
ID THS2_VITVI Reviewed; 392 AA.
AC P51070; A5BBF1;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 15-JAN-2008, sequence version 2.
DT 03-AUG-2022, entry version 130.
DE RecName: Full=Stilbene synthase 2;
DE EC=2.3.1.95;
DE AltName: Full=PSV21;
DE AltName: Full=Resveratrol synthase 2;
DE AltName: Full=Trihydroxystilbene synthase 2;
DE Short=StSy 2;
GN ORFNames=GSVIVT00004047001, LOC100246143, VITISV_010833;
GN and
GN ORFNames=GSVIVT00008253001, LOC100259169, VITISV_024260;
OS Vitis vinifera (Grape).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; Vitales; Vitaceae; Viteae; Vitis.
OX NCBI_TaxID=29760;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Optima;
RX PubMed=1898048; DOI=10.1016/0003-9861(91)90234-a;
RA Melchior F., Kindl H.;
RT "Coordinate- and elicitor-dependent expression of stilbene synthase and
RT phenylalanine ammonia-lyase genes in Vitis cv. Optima.";
RL Arch. Biochem. Biophys. 288:552-557(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Lambrusco Foglia Frastagliata;
RX PubMed=8193299; DOI=10.1007/bf00029856;
RA Sparvoli F., Martin C., Scienza A., Gavazzi G., Tonelli C.;
RT "Cloning and molecular analysis of structural genes involved in flavonoid
RT and stilbene biosynthesis in grape (Vitis vinifera L.).";
RL Plant Mol. Biol. 24:743-755(1994).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Pinot noir / PN40024;
RX PubMed=17721507; DOI=10.1038/nature06148;
RA Jaillon O., Aury J.-M., Noel B., Policriti A., Clepet C., Casagrande A.,
RA Choisne N., Aubourg S., Vitulo N., Jubin C., Vezzi A., Legeai F.,
RA Hugueney P., Dasilva C., Horner D., Mica E., Jublot D., Poulain J.,
RA Bruyere C., Billault A., Segurens B., Gouyvenoux M., Ugarte E.,
RA Cattonaro F., Anthouard V., Vico V., Del Fabbro C., Alaux M.,
RA Di Gaspero G., Dumas V., Felice N., Paillard S., Juman I., Moroldo M.,
RA Scalabrin S., Canaguier A., Le Clainche I., Malacrida G., Durand E.,
RA Pesole G., Laucou V., Chatelet P., Merdinoglu D., Delledonne M.,
RA Pezzotti M., Lecharny A., Scarpelli C., Artiguenave F., Pe M.E., Valle G.,
RA Morgante M., Caboche M., Adam-Blondon A.-F., Weissenbach J., Quetier F.,
RA Wincker P.;
RT "The grapevine genome sequence suggests ancestral hexaploidization in major
RT angiosperm phyla.";
RL Nature 449:463-467(2007).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Pinot noir;
RX DOI=10.1371/journal.pone.0001326;
RA Velasco R., Zharkikh A., Troggio M., Cartwright D.A., Cestaro A., Pruss D.,
RA Pindo M., FitzGerald L.M., Vezzulli S., Reid J., Malacarne G., Iliev D.,
RA Coppola G., Wardell B., Micheletti D., Macalma T., Facci M., Mitchell J.T.,
RA Perazzolli M., Eldredge G., Gatto P., Oyzerski R., Moretto M., Gutin N.,
RA Stefanini M., Chen Y., Segala C., Davenport C., Dematte L., Mraz A.,
RA Battilana J., Stormo K., Costa F., Tao Q., Si-Ammour A., Harkins T.,
RA Lackey A., Perbost C., Taillon B., Stella A., Solovyev V., Fawcett J.A.,
RA Sterck L., Vandepoele K., Grando S.M., Toppo S., Moser C., Lanchbury J.,
RA Bogden R., Skolnick M., Sgaramella V., Bhatnagar S.K., Fontana P.,
RA Gutin A., Van de Peer Y., Salamini F., Viola R.;
RT "A high quality draft consensus sequence of the genome of a heterozygous
RT grapevine variety.";
RL PLoS ONE 2:E1326-E1326(2007).
CC -!- FUNCTION: Mediates resistance to pathogens which are sensitive to
CC stilbenes. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=4-coumaroyl-CoA + 3 H(+) + 3 malonyl-CoA = 4 CO2 + 4 CoA +
CC trans-resveratrol; Xref=Rhea:RHEA:11936, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:16526, ChEBI:CHEBI:45713, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57355, ChEBI:CHEBI:57384; EC=2.3.1.95;
CC -!- PATHWAY: Phytoalexin biosynthesis; 3,4',5-trihydroxystilbene
CC biosynthesis; 3,4',5-trihydroxystilbene from trans-4-coumarate: step
CC 2/2.
CC -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm.
CC -!- INDUCTION: By stress.
CC -!- SIMILARITY: Belongs to the thiolase-like superfamily. Chalcone/stilbene
CC synthases family. {ECO:0000305}.
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DR EMBL; S63221; AAB19887.2; -; mRNA.
DR EMBL; X76892; CAA54221.1; -; mRNA.
DR EMBL; AM453354; CAN69968.1; -; Genomic_DNA.
DR EMBL; AM463938; CAN68069.1; -; Genomic_DNA.
DR RefSeq; XP_003634068.1; XM_003634020.3.
DR RefSeq; XP_019081580.1; XM_019226035.1.
DR AlphaFoldDB; P51070; -.
DR SMR; P51070; -.
DR STRING; 29760.VIT_16s0100g01010.t01; -.
DR GeneID; 100853406; -.
DR GeneID; 100855299; -.
DR KEGG; vvi:100853406; -.
DR eggNOG; ENOG502QRSY; Eukaryota.
DR HOGENOM; CLU_034992_2_0_1; -.
DR UniPathway; UPA00372; UER00548.
DR ExpressionAtlas; P51070; baseline and differential.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0050350; F:trihydroxystilbene synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0009058; P:biosynthetic process; IEA:InterPro.
DR GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR Gene3D; 3.40.47.10; -; 2.
DR InterPro; IPR012328; Chalcone/stilbene_synt_C.
DR InterPro; IPR001099; Chalcone/stilbene_synt_N.
DR InterPro; IPR018088; Chalcone/stilbene_synthase_AS.
DR InterPro; IPR011141; Polyketide_synthase_type-III.
DR InterPro; IPR016039; Thiolase-like.
DR PANTHER; PTHR11877; PTHR11877; 1.
DR Pfam; PF02797; Chal_sti_synt_C; 1.
DR Pfam; PF00195; Chal_sti_synt_N; 1.
DR PIRSF; PIRSF000451; PKS_III; 1.
DR SUPFAM; SSF53901; SSF53901; 2.
DR PROSITE; PS00441; CHALCONE_SYNTH; 1.
PE 2: Evidence at transcript level;
KW Acyltransferase; Cytoplasm; Plant defense; Stress response; Transferase.
FT CHAIN 1..392
FT /note="Stilbene synthase 2"
FT /id="PRO_0000216083"
FT ACT_SITE 164
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10023"
FT BINDING 55..58
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 267
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 305..307
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT CONFLICT 39
FT /note="Y -> F (in Ref. 2; CAA54221)"
FT /evidence="ECO:0000305"
FT CONFLICT 160..161
FT /note="YH -> DQ (in Ref. 1; AAB19887)"
FT /evidence="ECO:0000305"
FT CONFLICT 193
FT /note="I -> S (in Ref. 1; AAB19887)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 392 AA; 42758 MW; 30200ABC20660E86 CRC64;
MASVEEIRNA QRAKGPATIL AIGTATPDHC VYQSDYADYY FRVTKSEHMT ALKKKFNRIC
DKSMIKKRYI HLTEEMLEEH PNIGAYMAPS LNIRQEIITA EVPKLGKEAA LKALKEWGQP
KSKITHLVFC TTSGVEMPGA DYKLANLLGL EPSVRRVMLY HQGCYAGGTV LRTAKDLAEN
NAGARVLVVC SEITVVTFRG PSEDALDSLV GQALFGDGSA AVIVGSDPDI SIERPLFQLV
SAAQTFIPNS AGAIAGNLRE VGLTFHLWPN VPTLISENIE KCLTQAFDPL GISDWNSLFW
IAHPGGPAIL DAVEAKLNLD KKKLEATRHV LSEYGNMSSA CVLFILDEMR KKSLKGERAT
TGEGLDWGVL FGFGPGLTIE TVVLHSIPMV TN
