THS4_VITVI
ID THS4_VITVI Reviewed; 392 AA.
AC A5AEM3;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 12-JUN-2007, sequence version 1.
DT 03-AUG-2022, entry version 85.
DE RecName: Full=Stilbene synthase 4;
DE EC=2.3.1.95;
DE AltName: Full=Resveratrol synthase 4;
DE AltName: Full=Trihydroxystilbene synthase 4;
DE Short=StSy 4;
GN ORFNames=GSVIVT00005194001, LOC100241891, VITISV_031376;
OS Vitis vinifera (Grape).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; Vitales; Vitaceae; Viteae; Vitis.
OX NCBI_TaxID=29760;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Pinot noir / PN40024;
RX PubMed=17721507; DOI=10.1038/nature06148;
RA Jaillon O., Aury J.-M., Noel B., Policriti A., Clepet C., Casagrande A.,
RA Choisne N., Aubourg S., Vitulo N., Jubin C., Vezzi A., Legeai F.,
RA Hugueney P., Dasilva C., Horner D., Mica E., Jublot D., Poulain J.,
RA Bruyere C., Billault A., Segurens B., Gouyvenoux M., Ugarte E.,
RA Cattonaro F., Anthouard V., Vico V., Del Fabbro C., Alaux M.,
RA Di Gaspero G., Dumas V., Felice N., Paillard S., Juman I., Moroldo M.,
RA Scalabrin S., Canaguier A., Le Clainche I., Malacrida G., Durand E.,
RA Pesole G., Laucou V., Chatelet P., Merdinoglu D., Delledonne M.,
RA Pezzotti M., Lecharny A., Scarpelli C., Artiguenave F., Pe M.E., Valle G.,
RA Morgante M., Caboche M., Adam-Blondon A.-F., Weissenbach J., Quetier F.,
RA Wincker P.;
RT "The grapevine genome sequence suggests ancestral hexaploidization in major
RT angiosperm phyla.";
RL Nature 449:463-467(2007).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Pinot noir;
RX DOI=10.1371/journal.pone.0001326;
RA Velasco R., Zharkikh A., Troggio M., Cartwright D.A., Cestaro A., Pruss D.,
RA Pindo M., FitzGerald L.M., Vezzulli S., Reid J., Malacarne G., Iliev D.,
RA Coppola G., Wardell B., Micheletti D., Macalma T., Facci M., Mitchell J.T.,
RA Perazzolli M., Eldredge G., Gatto P., Oyzerski R., Moretto M., Gutin N.,
RA Stefanini M., Chen Y., Segala C., Davenport C., Dematte L., Mraz A.,
RA Battilana J., Stormo K., Costa F., Tao Q., Si-Ammour A., Harkins T.,
RA Lackey A., Perbost C., Taillon B., Stella A., Solovyev V., Fawcett J.A.,
RA Sterck L., Vandepoele K., Grando S.M., Toppo S., Moser C., Lanchbury J.,
RA Bogden R., Skolnick M., Sgaramella V., Bhatnagar S.K., Fontana P.,
RA Gutin A., Van de Peer Y., Salamini F., Viola R.;
RT "A high quality draft consensus sequence of the genome of a heterozygous
RT grapevine variety.";
RL PLoS ONE 2:E1326-E1326(2007).
CC -!- FUNCTION: Mediates resistance to pathogens which are sensitive to
CC stilbenes. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=4-coumaroyl-CoA + 3 H(+) + 3 malonyl-CoA = 4 CO2 + 4 CoA +
CC trans-resveratrol; Xref=Rhea:RHEA:11936, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:16526, ChEBI:CHEBI:45713, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57355, ChEBI:CHEBI:57384; EC=2.3.1.95;
CC -!- PATHWAY: Phytoalexin biosynthesis; 3,4',5-trihydroxystilbene
CC biosynthesis; 3,4',5-trihydroxystilbene from trans-4-coumarate: step
CC 2/2.
CC -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the thiolase-like superfamily. Chalcone/stilbene
CC synthases family. {ECO:0000305}.
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DR EMBL; AM424663; CAN82080.1; -; Genomic_DNA.
DR RefSeq; XP_003634073.1; XM_003634025.3.
DR AlphaFoldDB; A5AEM3; -.
DR SMR; A5AEM3; -.
DR STRING; 29760.VIT_16s0100g00900.t01; -.
DR GeneID; 100853675; -.
DR KEGG; vvi:100853675; -.
DR eggNOG; ENOG502QRSY; Eukaryota.
DR HOGENOM; CLU_034992_2_0_1; -.
DR OrthoDB; 950070at2759; -.
DR UniPathway; UPA00372; UER00548.
DR ExpressionAtlas; A5AEM3; baseline and differential.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0050350; F:trihydroxystilbene synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0009058; P:biosynthetic process; IEA:InterPro.
DR GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR Gene3D; 3.40.47.10; -; 2.
DR InterPro; IPR012328; Chalcone/stilbene_synt_C.
DR InterPro; IPR001099; Chalcone/stilbene_synt_N.
DR InterPro; IPR018088; Chalcone/stilbene_synthase_AS.
DR InterPro; IPR011141; Polyketide_synthase_type-III.
DR InterPro; IPR016039; Thiolase-like.
DR PANTHER; PTHR11877; PTHR11877; 1.
DR Pfam; PF02797; Chal_sti_synt_C; 1.
DR Pfam; PF00195; Chal_sti_synt_N; 1.
DR PIRSF; PIRSF000451; PKS_III; 1.
DR SUPFAM; SSF53901; SSF53901; 2.
DR PROSITE; PS00441; CHALCONE_SYNTH; 1.
PE 3: Inferred from homology;
KW Acyltransferase; Cytoplasm; Plant defense; Stress response; Transferase.
FT CHAIN 1..392
FT /note="Stilbene synthase 4"
FT /id="PRO_0000313087"
FT ACT_SITE 164
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10023"
FT BINDING 55..58
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 267
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 305..307
FT /ligand="substrate"
FT /evidence="ECO:0000250"
SQ SEQUENCE 392 AA; 42625 MW; 1A28CCF45DE357DF CRC64;
MASVEEIRNA QRAKGPATVL AIGTATPDNC LYQSDFADYY FRVTKSEHMT ELKKKFNRIC
DKSMIKKRYI HLTEEMLEEH PNIGAYMAPS LNIRQEIITA EVPKLGKEAA LKALKEWGQP
KSKITHLVFC TTSGVEMPGA DYKLANLLGL EPSVRRVMLY HQGCYAGGTV LRTAKDLAEN
NAGARVLVVC SEITVVTFRG PSEDALDSLV GQALFGDGSA AVIVGSDPDI SIERPLFQLV
SAAQTFIPNS AGAIAGNLRE VGLTFHLWPN VPTLISENIE NCLTKAFDPI GISDWNSLFW
IAHPGGPAIL DAVEAKVGLD KQKLKATRHI LSEYGNMSSA CVLFILDEMR KKSLKEGKTT
TGEGLDWGVL FGFGPGLTIE TVVLHSVGTD SN
