ID   THSA_BACAY              Reviewed;         297 AA.
AC   I2C645;
DT   03-AUG-2022, integrated into UniProtKB/Swiss-Prot.
DT   11-JUL-2012, sequence version 1.
DT   03-AUG-2022, entry version 30.
DE   RecName: Full=Thoeris protein ThsA {ECO:0000303|PubMed:29371424};
GN   Name=thsA {ECO:0000303|PubMed:29371424};
GN   ORFNames=MUS_2160 {ECO:0000312|EMBL:AFJ62119.1};
OS   Bacillus amyloliquefaciens (strain Y2) (Bacillus amyloliquefaciens subsp.
OS   plantarum (strain B9601-Y2)).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
OC   Bacillus amyloliquefaciens group.
OX   NCBI_TaxID=1126211;
RN   [1] {ECO:0000312|EMBL:AFJ62119.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Y2;
RX   PubMed=23357245; DOI=10.1016/j.jbiotec.2012.12.014;
RA   He P., Hao K., Blom J., Ruckert C., Vater J., Mao Z., Wu Y., Hou M., He P.,
RA   He Y., Borriss R.;
RT   "Genome sequence of the plant growth promoting strain Bacillus
RT   amyloliquefaciens subsp. plantarum B9601-Y2 and expression of mersacidin
RT   and other secondary metabolites.";
RL   J. Biotechnol. 164:281-291(2012).
RN   [2]
RP   FUNCTION IN ANTIVIRAL DEFENSE, DISRUPTION PHENOTYPE, MUTAGENESIS OF
RP   100-ASP--ASN-115, AND EXPRESSION IN B.SUBTILIS.
RC   STRAIN=Y2;
RX   PubMed=29371424; DOI=10.1126/science.aar4120;
RA   Doron S., Melamed S., Ofir G., Leavitt A., Lopatina A., Keren M.,
RA   Amitai G., Sorek R.;
RT   "Systematic discovery of antiphage defense systems in the microbial
RT   pangenome.";
RL   Science 359:0-0(2018).
CC   -!- FUNCTION: Probable membrane protein component of antiviral defense
CC       system Thoeris, composed of ThsA and ThsB. Expression of ThsA and ThsB
CC       in B.subtilis (strain BEST7003) confers resistance to phages SBSphiC,
CC       SBSphiJ and SPO1 (PubMed:29371424). Activation by a signal generated by
CC       ThsB leads to phage resistance (By similarity).
CC       {ECO:0000250|UniProtKB:J8G6Z1, ECO:0000269|PubMed:29371424}.
CC   -!- ACTIVITY REGULATION: Activated by a signal molecule generated by ThsB.
CC       {ECO:0000250|UniProtKB:J8G6Z1}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255}; Multi-pass membrane
CC       protein {ECO:0000255}.
CC   -!- DISRUPTION PHENOTYPE: When this gene is missing the Thoeris system does
CC       not confer phage resistance in B.subtilis.
CC       {ECO:0000269|PubMed:29371424}.
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DR   EMBL; CP003332; AFJ62119.1; -; Genomic_DNA.
DR   RefSeq; WP_014418039.1; NC_017912.1.
DR   SMR; I2C645; -.
DR   EnsemblBacteria; AFJ62119; AFJ62119; MUS_2160.
DR   KEGG; bqy:MUS_2160; -.
DR   PATRIC; fig|1126211.3.peg.2074; -.
DR   HOGENOM; CLU_083562_0_0_9; -.
DR   BioCyc; BAMY1126211:MUS_RS10315-MON; -.
DR   Proteomes; UP000002878; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   InterPro; IPR045535; DUF6430.
DR   Pfam; PF20016; DUF6430; 1.
PE   1: Evidence at protein level;
KW   Antiviral defense; Cell membrane; Membrane; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..297
FT                   /note="Thoeris protein ThsA"
FT                   /id="PRO_0000456256"
FT   TRANSMEM        32..52
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        57..77
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   MUTAGEN         100..115
FT                   /note="DIFEVPRNNYKVIAFN->AIFEVPRNNYKVIAFA: No longer
FT                   confers resistance to phage SBSphiJ."
FT                   /evidence="ECO:0000269|PubMed:29371424"
SQ   SEQUENCE   297 AA;  34693 MW;  69758A57B49D920F CRC64;
     MNIIGSDRNT NIGGFMWKFK ELIRDKTFRR WALSIILTIP TSVSTFISFL DLDARCRLII
     LLILVGLSLV IIIVQFIRLL FMNNITLNLN GSEVEIKKGD IFEVPRNNYK VIAFNEYFDT
     QVDDVIIARE TLNGQYIKRY YSHQDITELD QKIKDDVKLK IEEKNVERPF GGKTTRYSLG
     SVFKDMDFFL VAFSKFDREN RAQLKLNEYA SCMLNVWNEI NTLHASKEVF IPLLGSGITR
     HVDSDVGVNE LLHIMLWTFQ ISKVKFREPA KVTILLYKND HKKINFYKLK EFEKNGL