ID   THSB1_BACDA             Reviewed;         224 AA.
AC   A0A5B8Z670;
DT   03-AUG-2022, integrated into UniProtKB/Swiss-Prot.
DT   13-NOV-2019, sequence version 1.
DT   03-AUG-2022, entry version 9.
DE   RecName: Full=Thoeris protein TIR1 {ECO:0000303|PubMed:34853457};
GN   Name=thsB1 {ECO:0000305};
GN   ORFNames=FSZ17_06165 {ECO:0000312|EMBL:QED46886.1};
OS   Bacillus dafuensis.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=1742359;
RN   [1] {ECO:0000312|EMBL:QED46886.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=KCTC 43120 / FJAT-25496;
RA   Zheng X.;
RL   Submitted (AUG-2019) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   FUNCTION IN ANTIVIRAL DEFENSE, FUNCTION, ACTIVITY REGULATION, AND
RP   EXPRESSION IN B.SUBTILIS.
RC   STRAIN=KCTC 43120 / FJAT-25496;
RX   PubMed=34853457; DOI=10.1038/s41586-021-04098-7;
RA   Ofir G., Herbst E., Baroz M., Cohen D., Millman A., Doron S., Tal N.,
RA   Malheiro D.B.A., Malitsky S., Amitai G., Sorek R.;
RT   "Antiviral activity of bacterial TIR domains via immune signalling
RT   molecules.";
RL   Nature 600:116-120(2021).
CC   -!- FUNCTION: One of 2 TIR-like protein components of antiviral defense
CC       system Thoeris, composed of ThsA, TIR1 (thsB1) and TIR2 (thsB2). Phage
CC       infection activates this protein; by 70 minutes post-infection with
CC       phage SPO1, TIR1 generates a signal molecule that activates the NAD(+)
CC       hydrolase activity of ThsA (tested with B.cereus). The signal is
CC       similar to cyclic ADP-D-ribose, but how it differs is unknown.
CC       Expression of Thoeris in B.subtilis (strain BEST7003) confers
CC       resistance to phages phi29, phi3T, SPBeta, SBSphi11, SBSphi13, SBSphiJ,
CC       SPO1 and SPR but not SBSphiC. The TIR paralogs confer resistance to
CC       different phages; this subunit confers resistance to phi29, SBSphi11,
CC       SBSphi13, SBSphiJ, SPO1 and SPR but not phi3T, SBSphiC or SPBeta. There
CC       is overlap in the phage range for this system, both TIR1 and TIR2 are
CC       activated by SBSphi13, SBSphiJ, SPO1 and SPR.
CC       {ECO:0000269|PubMed:34853457}.
CC   -!- ACTIVITY REGULATION: Activated upon phage infection.
CC       {ECO:0000305|PubMed:34853457}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the Thoeris B TIR-like family. {ECO:0000305}.
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DR   EMBL; CP042593; QED46886.1; -; Genomic_DNA.
DR   RefSeq; WP_057775115.1; NZ_CP042593.1.
DR   KEGG; bda:FSZ17_06165; -.
DR   Proteomes; UP000321555; Chromosome.
DR   InterPro; IPR015032; MTH538_TIR-like_domain.
DR   Pfam; PF08937; DUF1863; 1.
PE   1: Evidence at protein level;
KW   Antiviral defense; Cytoplasm; Reference proteome.
FT   CHAIN           1..224
FT                   /note="Thoeris protein TIR1"
FT                   /id="PRO_0000456261"
SQ   SEQUENCE   224 AA;  26085 MW;  F46C563C880781B3 CRC64;
     MGRKIFISYK YSDSGVYPLN GNYSTTVRDY VDELQSKLKE GDHINKGEAD GEDLSEFKDE
     TIWTKLKDKI FDSSITIIFI SKNMKALFQS EEDQWIPWEI SYSLRELTRN GRTSGANALL
     AVVLPDQYNS YEYFIHDNSC PYCNCITLKT NTLFTILREN MFNIKSPTYN DCSNHSESNK
     VYTGESSYVR SVKWVDFIDN FDIYLQVAYN INENKDEYTL HKSV