THSBP_PSEAE
ID THSBP_PSEAE Reviewed; 332 AA.
AC Q9I3L9;
DT 23-FEB-2022, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 102.
DE RecName: Full=Thiosulfate-binding protein {ECO:0000305};
DE Flags: Precursor;
GN Name=cysP {ECO:0000312|EMBL:AAG04882.1};
GN OrderedLocusNames=PA1493 {ECO:0000312|EMBL:AAG04882.1};
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
RN [2]
RP FUNCTION AS A BINDING PROTEIN.
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=31627455; DOI=10.3390/ijms20205156;
RA Fernandez M., Rico-Jimenez M., Ortega A., Daddaoua A., Garcia Garcia A.I.,
RA Martin-Mora D., Torres N.M., Tajuelo A., Matilla M.A., Krell T.;
RT "Determination of Ligand Profiles for Pseudomonas aeruginosa Solute Binding
RT Proteins.";
RL Int. J. Mol. Sci. 20:5156-5156(2019).
CC -!- FUNCTION: Binds thiosulfate specifically and with high affinity. Has no
CC detectable affinity for sulfate. {ECO:0000269|PubMed:31627455}.
CC -!- SUBCELLULAR LOCATION: Periplasm {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the prokaryotic sulfate-binding protein family.
CC {ECO:0000305}.
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DR EMBL; AE004091; AAG04882.1; -; Genomic_DNA.
DR PIR; G83458; G83458.
DR RefSeq; NP_250184.1; NC_002516.2.
DR RefSeq; WP_003087175.1; NZ_QZGE01000005.1.
DR AlphaFoldDB; Q9I3L9; -.
DR SMR; Q9I3L9; -.
DR STRING; 287.DR97_529; -.
DR PaxDb; Q9I3L9; -.
DR PRIDE; Q9I3L9; -.
DR EnsemblBacteria; AAG04882; AAG04882; PA1493.
DR GeneID; 881623; -.
DR KEGG; pae:PA1493; -.
DR PATRIC; fig|208964.12.peg.1545; -.
DR PseudoCAP; PA1493; -.
DR HOGENOM; CLU_055615_0_1_6; -.
DR InParanoid; Q9I3L9; -.
DR OMA; TMNMATD; -.
DR PhylomeDB; Q9I3L9; -.
DR BioCyc; PAER208964:G1FZ6-1520-MON; -.
DR Proteomes; UP000002438; Chromosome.
DR GO; GO:0005615; C:extracellular space; IDA:PseudoCAP.
DR GO; GO:0042597; C:periplasmic space; IEA:UniProtKB-SubCell.
DR GO; GO:1902358; P:sulfate transmembrane transport; IEA:InterPro.
DR CDD; cd01005; PBP2_CysP; 1.
DR InterPro; IPR005669; Thiosulph/SO4-bd.
DR PANTHER; PTHR30368; PTHR30368; 1.
DR TIGRFAMs; TIGR00971; 3a0106s03; 1.
PE 1: Evidence at protein level;
KW Periplasm; Reference proteome; Signal; Transport.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT CHAIN 23..332
FT /note="Thiosulfate-binding protein"
FT /evidence="ECO:0000255"
FT /id="PRO_5004328472"
SQ SEQUENCE 332 AA; 36517 MW; 360A0F500AA45B27 CRC64;
MKRLFSASLL AAGLALGGAA HAAQPLLNVS YDVMRDFYKE YNPAFQKYWK AEKGENITIQ
MSHGGSSKQA RSVIDGLPAD VITMNQATDI DALADNGGLV PKDWATRLPN NSAPFTSATV
FIVRKGNPKA LKDWPDLLKD GVQVVVPNPK TSGNGRYTYL SAWGYVLKNG GDENKAKEFV
GKLFKQVPVL DTGGRAATTT FMQNQIGDVL VTFENEAEMI AREFGRGGFE VVYPSVSAEA
EPPVAVVDKV VEKKGSRAQA EAYLKYLWSD EGQTIAANNY LRPRNPEILA KFADRFPKVD
FFSVEKTFGD WRSVQKTHFI DGGVFDQIYS PN
