ID   THSB_BACAY              Reviewed;         190 AA.
AC   I2C644;
DT   03-AUG-2022, integrated into UniProtKB/Swiss-Prot.
DT   03-AUG-2022, sequence version 2.
DT   03-AUG-2022, entry version 29.
DE   RecName: Full=Thoeris protein ThsB {ECO:0000303|PubMed:29371424};
GN   Name=thsB {ECO:0000303|PubMed:29371424};
GN   ORFNames=MUS_2159 {ECO:0000312|EMBL:AFJ62118.1};
OS   Bacillus amyloliquefaciens (strain Y2) (Bacillus amyloliquefaciens subsp.
OS   plantarum (strain B9601-Y2)).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
OC   Bacillus amyloliquefaciens group.
OX   NCBI_TaxID=1126211;
RN   [1] {ECO:0000312|EMBL:AFJ62118.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Y2;
RX   PubMed=23357245; DOI=10.1016/j.jbiotec.2012.12.014;
RA   He P., Hao K., Blom J., Ruckert C., Vater J., Mao Z., Wu Y., Hou M., He P.,
RA   He Y., Borriss R.;
RT   "Genome sequence of the plant growth promoting strain Bacillus
RT   amyloliquefaciens subsp. plantarum B9601-Y2 and expression of mersacidin
RT   and other secondary metabolites.";
RL   J. Biotechnol. 164:281-291(2012).
RN   [2]
RP   FUNCTION IN ANTIVIRAL DEFENSE, DISRUPTION PHENOTYPE, MUTAGENESIS OF GLU-99,
RP   AND EXPRESSION IN B.SUBTILIS.
RC   STRAIN=Y2;
RX   PubMed=29371424; DOI=10.1126/science.aar4120;
RA   Doron S., Melamed S., Ofir G., Leavitt A., Lopatina A., Keren M.,
RA   Amitai G., Sorek R.;
RT   "Systematic discovery of antiphage defense systems in the microbial
RT   pangenome.";
RL   Science 359:0-0(2018).
CC   -!- FUNCTION: TIR-like domain-containing component of antiviral defense
CC       system Thoeris, composed of ThsA and ThsB. Expression of ThsA and ThsB
CC       in B.subtilis (strain BEST7003) confers resistance to phages SBSphiC,
CC       SBSphiJ and SPO1 (PubMed:29371424). Phage infection activates this
CC       protein, generating a signal molecule that in turn activates ThsA (By
CC       similarity). {ECO:0000250|UniProtKB:J8G8J6,
CC       ECO:0000269|PubMed:29371424}.
CC   -!- ACTIVITY REGULATION: Activated upon phage infection.
CC       {ECO:0000250|UniProtKB:J8G8J6}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- DISRUPTION PHENOTYPE: When this gene is missing the Thoeris system does
CC       not confer phage resistance in B.subtilis.
CC       {ECO:0000269|PubMed:29371424}.
CC   -!- SIMILARITY: Belongs to the Thoeris B TIR-like family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AFJ62118.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305|PubMed:29371424};
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DR   EMBL; CP003332; AFJ62118.1; ALT_INIT; Genomic_DNA.
DR   EnsemblBacteria; AFJ62118; AFJ62118; MUS_2159.
DR   KEGG; bqy:MUS_2159; -.
DR   PATRIC; fig|1126211.3.peg.2073; -.
DR   HOGENOM; CLU_120365_0_0_9; -.
DR   Proteomes; UP000002878; Chromosome.
DR   InterPro; IPR015032; MTH538_TIR-like_domain.
DR   Pfam; PF08937; DUF1863; 1.
PE   1: Evidence at protein level;
KW   Antiviral defense; Cytoplasm.
FT   CHAIN           1..190
FT                   /note="Thoeris protein ThsB"
FT                   /id="PRO_0000456259"
FT   MUTAGEN         99
FT                   /note="E->A: No longer confers resistance to phage
FT                   SBSphiJ."
FT                   /evidence="ECO:0000269|PubMed:29371424"
SQ   SEQUENCE   190 AA;  22002 MW;  61C4FFFFE87E86B4 CRC64;
     MGYRNGNYAA FYVSEPFSES SLGANATKDF VSYNMLRAWK GKDNNYPFND SHDKTYNVRD
     GSDWEKTLKP RLRKRLDQSK NIIFFLSKHT ENSKALREEI DYGINVKGLP VIVVYPELSE
     KSDIIDCTTK VFRSEVVNLW SRVPVFKDSM LKVPTIHIPY KKDQIKKALE NKDFMINSKI
     SAGSVYFYPC