ID   THST_THETS              Reviewed;         101 AA.
AC   Q9NBW4; P82355;
DT   01-JUN-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   25-MAY-2022, entry version 85.
DE   RecName: Full=Therostasin;
DE   Flags: Precursor;
OS   Theromyzon tessulatum (Duck leech).
OC   Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Annelida; Clitellata;
OC   Hirudinea; Rhynchobdellida; Glossiphoniidae; Theromyzon.
OX   NCBI_TaxID=13286;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 20-101, FUNCTION, TISSUE
RP   SPECIFICITY, AND MASS SPECTROMETRY.
RC   TISSUE=Head;
RX   PubMed=10852926; DOI=10.1074/jbc.m909217199;
RA   Chopin V., Salzet M., Baert J.-L., Vandenbulcke F., Sautiere P.-E.,
RA   Kerkaert J.-P., Malecha J.;
RT   "Therostasin, a novel clotting factor Xa inhibitor from the rhynchobdellid
RT   leech, Theromyzon tessulatum.";
RL   J. Biol. Chem. 275:32701-32707(2000).
CC   -!- FUNCTION: Potent inhibitor of factor Xa. It also inhibits trypsin in a
CC       weaker manner. {ECO:0000269|PubMed:10852926}.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Salivary glands. {ECO:0000269|PubMed:10852926}.
CC   -!- MASS SPECTROMETRY: Mass=8991; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:10852926};
CC   -!- SIMILARITY: Belongs to the protease inhibitor I15 (antistasin) family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AF239803; AAF73958.1; -; mRNA.
DR   AlphaFoldDB; Q9NBW4; -.
DR   SMR; Q9NBW4; -.
DR   MEROPS; I15.003; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR   GO; GO:0019229; P:regulation of vasoconstriction; IEA:InterPro.
DR   InterPro; IPR004094; Antistasin-like.
DR   InterPro; IPR019764; Endothelin_toxin_CS.
DR   InterPro; IPR011061; Hirudin/antistatin.
DR   Pfam; PF02822; Antistasin; 1.
DR   SUPFAM; SSF57262; SSF57262; 1.
DR   PROSITE; PS51252; ANTISTASIN; 2.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Protease inhibitor; Repeat; Secreted;
KW   Serine protease inhibitor; Signal.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000269|PubMed:10852926"
FT   CHAIN           20..101
FT                   /note="Therostasin"
FT                   /id="PRO_0000001701"
FT   DOMAIN          21..46
FT                   /note="Antistasin-like 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00582"
FT   DOMAIN          49..75
FT                   /note="Antistasin-like 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00582"
SQ   SEQUENCE   101 AA;  10951 MW;  8935C05F89AB8B98 CRC64;
     MRGLAVLLLV ACFCSVAFGD CENTECPRAC PGEYEFDEDG CNTCLCKGCN DAQCRIYCPL
     GFTTDANGCE SFCTCNTRET VCQNVVCSGK RVCNPRSGRC E