THT1_CAEEL
ID THT1_CAEEL Reviewed; 277 AA.
AC P91247;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1997, sequence version 1.
DT 03-AUG-2022, entry version 127.
DE RecName: Full=Putative thiosulfate sulfurtransferase mpst-4;
DE EC=2.8.1.1 {ECO:0000250|UniProtKB:P00586};
DE AltName: Full=Mercaptopyruvate sulfurtransferase homolog 4 {ECO:0000312|WormBase:F11G11.9};
GN Name=mpst-4 {ECO:0000312|WormBase:F11G11.9};
GN ORFNames=F11G11.9 {ECO:0000312|WormBase:F11G11.9};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2]
RP IDENTIFICATION.
RX PubMed=22131987; DOI=10.1155/2011/394970;
RA Mathew N.D., Schlipalius D.I., Ebert P.R.;
RT "Sulfurous gases as biological messengers and toxins: comparative genetics
RT of their metabolism in model organisms.";
RL J. Toxicol. 2011:394970-394984(2011).
RN [3]
RP INDUCTION.
RX PubMed=24093496; DOI=10.1089/ars.2013.5448;
RA Qabazard B., Li L., Gruber J., Peh M.T., Ng L.F., Kumar S.D., Rose P.,
RA Tan C.H., Dymock B.W., Wei F., Swain S.C., Halliwell B., Sturzenbaum S.R.,
RA Moore P.K.;
RT "Hydrogen sulfide is an endogenous regulator of aging in Caenorhabditis
RT elegans.";
RL Antioxid. Redox Signal. 20:2621-2630(2014).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=hydrogen cyanide + thiosulfate = 2 H(+) + sulfite +
CC thiocyanate; Xref=Rhea:RHEA:16881, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:17359, ChEBI:CHEBI:18022, ChEBI:CHEBI:18407,
CC ChEBI:CHEBI:33542; EC=2.8.1.1;
CC Evidence={ECO:0000250|UniProtKB:P00586};
CC -!- INDUCTION: Expression increased by knockdown of mpst-1.
CC {ECO:0000269|PubMed:24093496}.
CC -!- DOMAIN: Contains two rhodanese domains with different primary
CC structures but with near identical secondary structure conformations
CC suggesting a common evolutionary origin. Only the C-terminal rhodanese
CC domain contains the catalytic cysteine residue.
CC {ECO:0000250|UniProtKB:P00586}.
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DR EMBL; FO081119; CCD69260.1; -; Genomic_DNA.
DR PIR; T29979; T29979.
DR RefSeq; NP_001309500.1; NM_001322727.1.
DR AlphaFoldDB; P91247; -.
DR SMR; P91247; -.
DR STRING; 6239.F11G11.9; -.
DR PaxDb; P91247; -.
DR PeptideAtlas; P91247; -.
DR EnsemblMetazoa; F11G11.9.1; F11G11.9.1; WBGene00017387.
DR GeneID; 173838; -.
DR KEGG; cel:CELE_F11G11.9; -.
DR UCSC; F11G11.9; c. elegans.
DR CTD; 173838; -.
DR WormBase; F11G11.9; CE51449; WBGene00017387; mpst-4.
DR eggNOG; KOG1529; Eukaryota.
DR GeneTree; ENSGT00510000046773; -.
DR HOGENOM; CLU_076711_0_0_1; -.
DR InParanoid; P91247; -.
DR OMA; AIVIKIE; -.
DR OrthoDB; 1553525at2759; -.
DR PhylomeDB; P91247; -.
DR PRO; PR:P91247; -.
DR Proteomes; UP000001940; Chromosome II.
DR Bgee; WBGene00017387; Expressed in material anatomical entity and 2 other tissues.
DR GO; GO:0005739; C:mitochondrion; IBA:GO_Central.
DR GO; GO:0004792; F:thiosulfate sulfurtransferase activity; IBA:GO_Central.
DR GO; GO:0019346; P:transsulfuration; IBA:GO_Central.
DR Gene3D; 3.40.250.10; -; 2.
DR InterPro; IPR001763; Rhodanese-like_dom.
DR InterPro; IPR036873; Rhodanese-like_dom_sf.
DR InterPro; IPR045078; TST/MPST-like.
DR PANTHER; PTHR11364; PTHR11364; 2.
DR Pfam; PF00581; Rhodanese; 1.
DR SMART; SM00450; RHOD; 1.
DR SUPFAM; SSF52821; SSF52821; 2.
DR PROSITE; PS50206; RHODANESE_3; 2.
PE 2: Evidence at transcript level;
KW Reference proteome; Repeat; Transferase.
FT CHAIN 1..277
FT /note="Putative thiosulfate sulfurtransferase mpst-4"
FT /id="PRO_0000139403"
FT DOMAIN 15..153
FT /note="Rhodanese 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00173"
FT DOMAIN 155..243
FT /note="Rhodanese 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00173"
FT ACT_SITE 204
FT /note="Cysteine persulfide intermediate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00173"
SQ SEQUENCE 277 AA; 30699 MW; 25EDF666CE12824C CRC64;
MAVDMIVEPK WVVQNFGNIR ILDASWTFKP KADVAEYKAK YYNKFGVGMN ELKNPEYLAE
HINGAAHFNF DIAYYPSEDE RFTLYTPEEF SSYVKRLGVF NGDHLVIYGR GKDGGMAAAS
RAYWTFRYYG YTTVSVLNGG IEAFKLAQGV VQSDSKAEGI RCKDAIHFPI GEVCAAKGFK
KKTDCDQAFA AKGIKVGDTV VISCGIGLSA SAICLAAARS GIVAKLYNGG VHELAYKAPQ
HLNMRVTLLL HAITVLRCTY IHFTFLYAIV IKIERIV
