THTR_METTH
ID THTR_METTH Reviewed; 286 AA.
AC O26719;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 25-MAY-2022, entry version 103.
DE RecName: Full=Putative thiosulfate sulfurtransferase;
DE EC=2.8.1.1;
GN OrderedLocusNames=MTH_622;
OS Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM
OS 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum).
OC Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
OC Methanobacteriales; Methanobacteriaceae; Methanothermobacter.
OX NCBI_TaxID=187420;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H;
RX PubMed=9371463; DOI=10.1128/jb.179.22.7135-7155.1997;
RA Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J.,
RA Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D.,
RA Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R.,
RA Wang Y., Wierzbowski J., Gibson R., Jiwani N., Caruso A., Bush D.,
RA Safer H., Patwell D., Prabhakar S., McDougall S., Shimer G., Goyal A.,
RA Pietrovski S., Church G.M., Daniels C.J., Mao J.-I., Rice P., Noelling J.,
RA Reeve J.N.;
RT "Complete genome sequence of Methanobacterium thermoautotrophicum deltaH:
RT functional analysis and comparative genomics.";
RL J. Bacteriol. 179:7135-7155(1997).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=hydrogen cyanide + thiosulfate = 2 H(+) + sulfite +
CC thiocyanate; Xref=Rhea:RHEA:16881, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:17359, ChEBI:CHEBI:18022, ChEBI:CHEBI:18407,
CC ChEBI:CHEBI:33542; EC=2.8.1.1;
CC -!- DOMAIN: Contains two rhodanese domains with different primary
CC structures but with near identical secondary structure conformations
CC suggesting a common evolutionary origin. Only the C-terminal rhodanese
CC domain contains the catalytic cysteine residue (By similarity).
CC {ECO:0000250}.
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DR EMBL; AE000666; AAB85128.1; -; Genomic_DNA.
DR PIR; G69182; G69182.
DR RefSeq; WP_010876261.1; NC_000916.1.
DR AlphaFoldDB; O26719; -.
DR SMR; O26719; -.
DR STRING; 187420.MTH_622; -.
DR EnsemblBacteria; AAB85128; AAB85128; MTH_622.
DR GeneID; 1470583; -.
DR KEGG; mth:MTH_622; -.
DR PATRIC; fig|187420.15.peg.603; -.
DR HOGENOM; CLU_031618_1_7_2; -.
DR OMA; EGSLTEW; -.
DR Proteomes; UP000005223; Chromosome.
DR GO; GO:0004792; F:thiosulfate sulfurtransferase activity; IEA:UniProtKB-EC.
DR Gene3D; 3.40.250.10; -; 2.
DR InterPro; IPR001763; Rhodanese-like_dom.
DR InterPro; IPR036873; Rhodanese-like_dom_sf.
DR InterPro; IPR001307; Thiosulphate_STrfase_CS.
DR Pfam; PF00581; Rhodanese; 2.
DR SMART; SM00450; RHOD; 2.
DR SUPFAM; SSF52821; SSF52821; 2.
DR PROSITE; PS00380; RHODANESE_1; 1.
DR PROSITE; PS00683; RHODANESE_2; 1.
DR PROSITE; PS50206; RHODANESE_3; 2.
PE 3: Inferred from homology;
KW Reference proteome; Repeat; Transferase.
FT CHAIN 1..286
FT /note="Putative thiosulfate sulfurtransferase"
FT /id="PRO_0000139421"
FT DOMAIN 22..137
FT /note="Rhodanese 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00173"
FT DOMAIN 167..280
FT /note="Rhodanese 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00173"
FT ACT_SITE 239
FT /note="Cysteine persulfide intermediate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00173"
SQ SEQUENCE 286 AA; 32165 MW; AE8E020D0DECD523 CRC64;
MEPYGKGDGR VRWVTPEWLM ENMEDVAIID CQPNIHDYIL EHIPGAVYLN EGLFREPRGK
APAMYIPEGA VELIFQQAGI ENRPTVVYTG TGGVKGWGDG LEQTMVAYSL ARFGHENILV
LNGGLAEWKR AGGELTKVFP EVEESGFSAV TKEDFYIEYP EFKRIKDDED VLLLDARPAE
VYEGQGPWIK PGHIPGAVNL PWADLMDPEN RTLLKPEDEI LELVNSVGAT PDRKIICSCG
TGREATNEFL LFRWYLGYPD VRIYEGSFTE WTQIEDNPTV TGPDPR
