THTR_MYCTU
ID THTR_MYCTU Reviewed; 277 AA.
AC P9WHF9; L0TBW4; O05793;
DT 16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT 16-APR-2014, sequence version 1.
DT 03-AUG-2022, entry version 34.
DE RecName: Full=Putative thiosulfate sulfurtransferase;
DE EC=2.8.1.1;
DE AltName: Full=Rhodanese-like protein;
GN Name=cysA1; Synonyms=cysA; OrderedLocusNames=Rv3117; ORFNames=MTCY164.27;
GN and
GN Name=cysA2; OrderedLocusNames=Rv0815c; ORFNames=MTV043.07c;
OS Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycobacterium; Mycobacterium tuberculosis complex.
OX NCBI_TaxID=83332;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=9634230; DOI=10.1038/31159;
RA Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA Barrell B.G.;
RT "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT genome sequence.";
RL Nature 393:537-544(1998).
RN [2]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT mass spectrometry.";
RL Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
CC -!- FUNCTION: May be a sulfotransferase involved in the formation of
CC thiosulfate. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=hydrogen cyanide + thiosulfate = 2 H(+) + sulfite +
CC thiocyanate; Xref=Rhea:RHEA:16881, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:17359, ChEBI:CHEBI:18022, ChEBI:CHEBI:18407,
CC ChEBI:CHEBI:33542; EC=2.8.1.1;
CC -!- DOMAIN: Contains two rhodanese domains with different primary
CC structures but with near identical secondary structure conformations
CC suggesting a common evolutionary origin. Only the C-terminal rhodanese
CC domain contains the catalytic cysteine residue (By similarity).
CC {ECO:0000250}.
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DR EMBL; AL123456; CCP43563.1; -; Genomic_DNA.
DR EMBL; AL123456; CCP45927.1; -; Genomic_DNA.
DR PIR; G70809; G70809.
DR RefSeq; NP_215330.1; NC_000962.3.
DR RefSeq; NP_217633.1; NC_000962.3.
DR RefSeq; WP_003404293.1; NZ_NVQJ01000118.1.
DR PDB; 3AAX; X-ray; 2.50 A; A/B=1-277.
DR PDB; 3AAY; X-ray; 1.90 A; A/B=1-277.
DR PDB; 3HWI; X-ray; 2.29 A; A/B=1-277.
DR PDBsum; 3AAX; -.
DR PDBsum; 3AAY; -.
DR PDBsum; 3HWI; -.
DR AlphaFoldDB; P9WHF9; -.
DR SMR; P9WHF9; -.
DR STRING; 83332.Rv0815c; -.
DR PaxDb; P9WHF9; -.
DR DNASU; 23493067; -.
DR DNASU; 885449; -.
DR DNASU; 888802; -.
DR GeneID; 885449; -.
DR GeneID; 888802; -.
DR KEGG; mtu:Rv0815c; -.
DR KEGG; mtu:Rv3117; -.
DR TubercuList; Rv0815c; -.
DR eggNOG; COG2897; Bacteria.
DR OMA; QRPGHVP; -.
DR PhylomeDB; P9WHF9; -.
DR BRENDA; 2.8.1.1; 3445.
DR BRENDA; 2.8.1.2; 3445.
DR Proteomes; UP000001584; Chromosome.
DR GO; GO:0005829; C:cytosol; HDA:MTBBASE.
DR GO; GO:0009274; C:peptidoglycan-based cell wall; HDA:MTBBASE.
DR GO; GO:0005886; C:plasma membrane; HDA:MTBBASE.
DR GO; GO:0004792; F:thiosulfate sulfurtransferase activity; IEA:UniProtKB-EC.
DR Gene3D; 3.40.250.10; -; 2.
DR InterPro; IPR001763; Rhodanese-like_dom.
DR InterPro; IPR036873; Rhodanese-like_dom_sf.
DR InterPro; IPR001307; Thiosulphate_STrfase_CS.
DR Pfam; PF00581; Rhodanese; 2.
DR SMART; SM00450; RHOD; 2.
DR SUPFAM; SSF52821; SSF52821; 2.
DR PROSITE; PS00683; RHODANESE_2; 1.
DR PROSITE; PS50206; RHODANESE_3; 2.
PE 1: Evidence at protein level;
KW 3D-structure; Reference proteome; Repeat; Transferase.
FT CHAIN 1..277
FT /note="Putative thiosulfate sulfurtransferase"
FT /id="PRO_0000139415"
FT DOMAIN 18..125
FT /note="Rhodanese 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00173"
FT DOMAIN 154..274
FT /note="Rhodanese 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00173"
FT ACT_SITE 233
FT /note="Cysteine persulfide intermediate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00173"
FT BINDING 238
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT HELIX 3..6
FT /evidence="ECO:0007829|PDB:3AAY"
FT HELIX 10..14
FT /evidence="ECO:0007829|PDB:3AAY"
FT TURN 15..18
FT /evidence="ECO:0007829|PDB:3AAY"
FT STRAND 22..31
FT /evidence="ECO:0007829|PDB:3AAY"
FT HELIX 32..36
FT /evidence="ECO:0007829|PDB:3AAY"
FT STRAND 43..46
FT /evidence="ECO:0007829|PDB:3AAY"
FT TURN 47..51
FT /evidence="ECO:0007829|PDB:3AAY"
FT STRAND 54..59
FT /evidence="ECO:0007829|PDB:3AAY"
FT HELIX 62..72
FT /evidence="ECO:0007829|PDB:3AAY"
FT STRAND 78..83
FT /evidence="ECO:0007829|PDB:3AAY"
FT HELIX 86..88
FT /evidence="ECO:0007829|PDB:3AAY"
FT HELIX 89..100
FT /evidence="ECO:0007829|PDB:3AAY"
FT STRAND 105..109
FT /evidence="ECO:0007829|PDB:3AAY"
FT HELIX 112..118
FT /evidence="ECO:0007829|PDB:3AAY"
FT HELIX 142..144
FT /evidence="ECO:0007829|PDB:3AAY"
FT HELIX 148..153
FT /evidence="ECO:0007829|PDB:3AAY"
FT TURN 154..157
FT /evidence="ECO:0007829|PDB:3AAY"
FT STRAND 158..162
FT /evidence="ECO:0007829|PDB:3AAY"
FT HELIX 166..169
FT /evidence="ECO:0007829|PDB:3AAY"
FT STRAND 174..177
FT /evidence="ECO:0007829|PDB:3AAX"
FT STRAND 184..186
FT /evidence="ECO:0007829|PDB:3AAX"
FT HELIX 198..201
FT /evidence="ECO:0007829|PDB:3AAY"
FT HELIX 211..221
FT /evidence="ECO:0007829|PDB:3AAY"
FT STRAND 229..232
FT /evidence="ECO:0007829|PDB:3AAY"
FT HELIX 236..247
FT /evidence="ECO:0007829|PDB:3AAY"
FT STRAND 255..260
FT /evidence="ECO:0007829|PDB:3AAY"
FT HELIX 261..265
FT /evidence="ECO:0007829|PDB:3AAY"
SQ SEQUENCE 277 AA; 31015 MW; AC37B715D99565A9 CRC64;
MARCDVLVSA DWAESNLHAP KVVFVEVDED TSAYDRDHIA GAIKLDWRTD LQDPVKRDFV
DAQQFSKLLS ERGIANEDTV ILYGGNNNWF AAYAYWYFKL YGHEKVKLLD GGRKKWELDG
RPLSSDPVSR PVTSYTASPP DNTIRAFRDE VLAAINVKNL IDVRSPDEFS GKILAPAHLP
QEQSQRPGHI PGAINVPWSR AANEDGTFKS DEELAKLYAD AGLDNSKETI AYCRIGERSS
HTWFVLRELL GHQNVKNYDG SWTEYGSLVG APIELGS
