ID   THUM3_BOVIN             Reviewed;         506 AA.
AC   Q2T9W2; Q1JPF6;
DT   14-NOV-2006, integrated into UniProtKB/Swiss-Prot.
DT   24-JAN-2006, sequence version 1.
DT   03-AUG-2022, entry version 67.
DE   RecName: Full=tRNA (guanine(6)-N2)-methyltransferase THUMP3 {ECO:0000305};
DE            EC=2.1.1.256 {ECO:0000250|UniProtKB:Q9BV44};
DE   AltName: Full=THUMP domain-containing protein 3 {ECO:0000250|UniProtKB:Q9BV44};
GN   Name=THUMPD3;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Crossbred X Angus; TISSUE=Liver;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (DEC-2005) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-214.
RX   PubMed=16305752; DOI=10.1186/1471-2164-6-166;
RA   Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L.,
RA   Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L.;
RT   "Characterization of 954 bovine full-CDS cDNA sequences.";
RL   BMC Genomics 6:166-166(2005).
CC   -!- FUNCTION: Methyltransferase which catalyzes the formation of N(2)-
CC       methylguanosine at position 6 in a broad range of tRNA substrates
CC       containing the characteristic 3'-CCA terminus of mature tRNAs (By
CC       similarity). Also catalyzes the formation of N(2)-methylguanosine at
CC       position 7 of tRNA(Trp) (By similarity). Requires the methyltransferase
CC       adapter protein TRM112 for tRNA methyltransferase activity (By
CC       similarity). {ECO:0000250|UniProtKB:Q9BV44}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=guanosine(6) in tRNA + S-adenosyl-L-methionine = H(+) + N(2)-
CC         methylguanosine(6) in tRNA + S-adenosyl-L-homocysteine;
CC         Xref=Rhea:RHEA:51116, Rhea:RHEA-COMP:12888, Rhea:RHEA-COMP:12889,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC         ChEBI:CHEBI:74269, ChEBI:CHEBI:74481; EC=2.1.1.256;
CC         Evidence={ECO:0000250|UniProtKB:Q9BV44};
CC   -!- SUBUNIT: Interacts with TRMT112; the interaction is direct and is
CC       required for THUMPD3 methyltransferase activity.
CC       {ECO:0000250|UniProtKB:Q9BV44}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q9BV44}.
CC   -!- SIMILARITY: Belongs to the methyltransferase superfamily.
CC       {ECO:0000305}.
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DR   EMBL; BC111239; AAI11240.1; -; mRNA.
DR   EMBL; BT025397; ABF57353.1; -; mRNA.
DR   RefSeq; NP_001033151.1; NM_001038062.1.
DR   AlphaFoldDB; Q2T9W2; -.
DR   SMR; Q2T9W2; -.
DR   STRING; 9913.ENSBTAP00000004047; -.
DR   PaxDb; Q2T9W2; -.
DR   PRIDE; Q2T9W2; -.
DR   GeneID; 508940; -.
DR   KEGG; bta:508940; -.
DR   CTD; 25917; -.
DR   eggNOG; ENOG502QSE5; Eukaryota.
DR   InParanoid; Q2T9W2; -.
DR   OrthoDB; 674030at2759; -.
DR   Proteomes; UP000009136; Unplaced.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0000049; F:tRNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR   GO; GO:0008033; P:tRNA processing; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.150; -; 1.
DR   InterPro; IPR000241; RNA_methylase_dom.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   InterPro; IPR004114; THUMP_dom.
DR   Pfam; PF02926; THUMP; 1.
DR   Pfam; PF01170; UPF0020; 1.
DR   SMART; SM00981; THUMP; 1.
DR   SUPFAM; SSF53335; SSF53335; 1.
DR   PROSITE; PS51165; THUMP; 1.
DR   PROSITE; PS01261; UPF0020; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Methyltransferase; Reference proteome; RNA-binding;
KW   S-adenosyl-L-methionine; Transferase; tRNA processing; tRNA-binding.
FT   CHAIN           1..506
FT                   /note="tRNA (guanine(6)-N2)-methyltransferase THUMP3"
FT                   /id="PRO_0000259768"
FT   DOMAIN          170..286
FT                   /note="THUMP"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00529"
FT   REGION          144..172
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CONFLICT        79
FT                   /note="T -> S (in Ref. 2; ABF57353)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   506 AA;  56674 MW;  95BD53AE9B36B71B CRC64;
     MSDVQEATNQ LLDVNLCENQ MSIQVTESGP RSESEHLQVT IGATVPTGFE QTAADEVREK
     LGSSCKISKD RGKIYFDITV DSLAQVHCLR SVDNLFVVVQ EFKDYQFKET KEEVLKDFEE
     LAGKLPWSDP LKIWKINTCF KKKKTKRRKL NPNSSKQKID NGRGDTTVEK DVKKELTNSV
     SDSQISDYYE NPAIKEQVST LIGDGLTSCK DETEENSKEE ADPEVLKFRV TCNRAGEKHC
     FSSNEAARDF GGAVQDYFKW KADMTNFDVE VLLNIHDNEV VVGIALTEES LHRRNITHFG
     PTTLRSTLAY GMLRLCAPQP TDIIVDPMCG TGAIPIEGAT EWSNCYHIAG DNNPLAVNRA
     ANNISSLLTK IQVKEGKLPL GLPIDTIQWD ICNLPLRTGS VDIIVTDMPF GKRMGSKKRN
     WNLYPACLRE MSRVCRPGTG RAVLLTQDKK CFAKALSGMG HLWRKVHTVW VNIGGLHAAV
     YLLKRTPQSF VHPSEEDGER CPCTQR