ID   BRXA_ECOHS              Reviewed;         213 AA.
AC   P0DUF6; A0A7M3S2P1;
DT   02-JUN-2021, integrated into UniProtKB/Swiss-Prot.
DT   02-JUN-2021, sequence version 1.
DT   25-MAY-2022, entry version 4.
DE   RecName: Full=BREX protein BrxA {ECO:0000303|PubMed:30418590};
GN   Name=brxA {ECO:0000303|PubMed:30418590}; OrderedLocusNames=EcHS_A0336;
OS   Escherichia coli O9:H4 (strain HS).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=331112;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=HS;
RX   PubMed=18676672; DOI=10.1128/jb.00619-08;
RA   Rasko D.A., Rosovitz M.J., Myers G.S.A., Mongodin E.F., Fricke W.F.,
RA   Gajer P., Crabtree J., Sebaihia M., Thomson N.R., Chaudhuri R.,
RA   Henderson I.R., Sperandio V., Ravel J.;
RT   "The pangenome structure of Escherichia coli: comparative genomic analysis
RT   of E. coli commensal and pathogenic isolates.";
RL   J. Bacteriol. 190:6881-6893(2008).
RN   [2]
RP   FUNCTION, INDUCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=HS;
RX   PubMed=30418590; DOI=10.1093/nar/gky1125;
RA   Gordeeva J., Morozova N., Sierro N., Isaev A., Sinkunas T., Tsvetkova K.,
RA   Matlashov M., Truncaite L., Morgan R.D., Ivanov N.V., Siksnys V., Zeng L.,
RA   Severinov K.;
RT   "BREX system of Escherichia coli distinguishes self from non-self by
RT   methylation of a specific DNA site.";
RL   Nucleic Acids Res. 47:253-265(2019).
CC   -!- FUNCTION: BREX systems (bacteriophage exclusion) provide immunity
CC       against bacteriophage. A probably non-essential part of a type 1 BREX
CC       system which protects against dsDNA phage. This system allows phage
CC       adsorption but prevents phage DNA replication, without degradation of
CC       the phage DNA. Methylation of bacterial DNA by PglX guides self/non-
CC       self discrimination. When the brxA-brxB-brxC-pglX-pglZ-brxL genes are
CC       transformed into a susceptible E.coli strain (BW25113) they confer very
CC       high resistance to infection by bacteriophage VR7 and VpaE1, about 100-
CC       fold protection against lambda, T5 and T7 and no protection against RNA
CC       phage Qbeta, ssDNA phage M13 or dSDNA phage T4 and VR5. Glycosylated
CC       phage DNA is not susceptible to BREX. The BREX system does not confer
CC       resistance to lysogenic lambda phage, i.e. prophage that are integrated
CC       into the chromosomal DNA and then induced to form phage.
CC       {ECO:0000269|PubMed:30418590}.
CC   -!- INDUCTION: Transcribed at slowly increasing levels as cells progress
CC       from lag to exponential to stationary phase.
CC       {ECO:0000269|PubMed:30418590}.
CC   -!- DISRUPTION PHENOTYPE: No visible phenotype, host DNA is methylated on
CC       fifth position of 5'-GGTAAG-3' in chromosomal DNA, BREX still confers
CC       phage resistance. {ECO:0000269|PubMed:30418590}.
CC   -!- SIMILARITY: Belongs to the BrxA family. {ECO:0000305}.
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DR   EMBL; CP000802; ABV04724.1; -; Genomic_DNA.
DR   AlphaFoldDB; P0DUF6; -.
DR   SMR; P0DUF6; -.
DR   KEGG; ecx:EcHS_A0336; -.
DR   OMA; FYKLKQV; -.
DR   Proteomes; UP000001123; Chromosome.
DR   GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.3540.10; -; 1.
DR   InterPro; IPR014948; BrxA.
DR   InterPro; IPR023137; BrxA_sf.
DR   Pfam; PF08849; DUF1819; 1.
PE   2: Evidence at transcript level;
KW   Antiviral defense.
FT   CHAIN           1..213
FT                   /note="BREX protein BrxA"
FT                   /id="PRO_0000452153"
SQ   SEQUENCE   213 AA;  23999 MW;  E0206DF190E696FA CRC64;
     MRAAFQSSEG FSMIKNDKAW IGDLLGGPLM SRESRVIAEL LLTDPDEQTW QEQIVGHNIL
     QASSPNTAKR YAATIRLRLN TLDKSAWTLI AEGSERERQQ LLFVALMLHS PVVKDFLAEV
     VNDLRRQFKE KLPGNSWNEF VNSQVRLHPV LASYSDSSIA KMGNNLVKAL AEAGYVDTPR
     RRNLQAVYLL PETQAVLQRL GQQDLISILE GKR