THYN1_HUMAN
ID THYN1_HUMAN Reviewed; 225 AA.
AC Q9P016; Q567Q2; Q9H3L4; Q9HC20;
DT 28-NOV-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 143.
DE RecName: Full=Thymocyte nuclear protein 1;
DE AltName: Full=Thymocyte protein Thy28;
GN Name=THYN1; Synonyms=THY28; ORFNames=HSPC144, MDS012, My0054;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RA Ge H.P., Yu L., Cui W.C., Fan Y.X., Yang Y.M., Zhao S.Y.;
RT "Cloning and characterization of a novel human cDNA homology to chicken
RT thymocyte protein cThy28kD mRNA.";
RL Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Fetal brain;
RA Mao Y.M., Xie Y., Zhou Z.X.;
RL Submitted (APR-1998) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RA Huang C., Qian B., Tu Y., Gu W., Wang Y., Han Z., Chen Z.;
RT "Novel genes expressed in hematopoietic stem/progenitor cells from
RT myelodysplastic syndrome patients.";
RL Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Umbilical cord blood;
RX PubMed=11042152; DOI=10.1101/gr.140200;
RA Zhang Q.-H., Ye M., Wu X.-Y., Ren S.-X., Zhao M., Zhao C.-J., Fu G.,
RA Shen Y., Fan H.-Y., Lu G., Zhong M., Xu X.-R., Han Z.-G., Zhang J.-W.,
RA Tao J., Huang Q.-H., Zhou J., Hu G.-X., Gu J., Chen S.-J., Chen Z.;
RT "Cloning and functional analysis of cDNAs with open reading frames for 300
RT previously undefined genes expressed in CD34+ hematopoietic stem/progenitor
RT cells.";
RL Genome Res. 10:1546-1560(2000).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC TISSUE=Spinal cord, and Urinary bladder;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP PURIFICATION.
RX PubMed=15939300; DOI=10.1016/j.pep.2005.03.008;
RA Song A.X., Chang Y.G., Gao Y.G., Lin X.J., Shi Y.H., Lin D.H., Hang Q.H.,
RA Hu H.Y.;
RT "Identification, expression, and purification of a unique stable domain
RT from human HSPC144 protein.";
RL Protein Expr. Purif. 42:146-152(2005).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T.,
RA Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [8]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=24275569; DOI=10.1016/j.jprot.2013.11.014;
RA Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L.,
RA Ye M., Zou H.;
RT "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver
RT phosphoproteome.";
RL J. Proteomics 96:253-262(2014).
RN [9]
RP X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 55-221.
RX PubMed=19237743; DOI=10.1107/s0907444908041474;
RA Yu F., Song A., Xu C., Sun L., Li J., Tang L., Yu M., Yeates T.O., Hu H.,
RA He J.;
RT "Determining the DUF55-domain structure of human thymocyte nuclear protein
RT 1 from crystals partially twinned by tetartohedry.";
RL Acta Crystallogr. D 65:212-219(2009).
CC -!- FUNCTION: Specifically binds 5-hydroxymethylcytosine (5hmC), suggesting
CC that it acts as a specific reader of 5hmC. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9P016-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9P016-2; Sequence=VSP_021789;
CC -!- PTM: Phosphorylated. {ECO:0000250}.
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DR EMBL; AF087886; AAP97185.1; -; mRNA.
DR EMBL; AF059619; AAG43118.1; -; mRNA.
DR EMBL; AF182413; AAG14949.1; -; mRNA.
DR EMBL; AF161493; AAF29108.1; -; mRNA.
DR EMBL; BC006978; AAH06978.1; -; mRNA.
DR EMBL; BC093074; AAH93074.1; -; mRNA.
DR CCDS; CCDS8496.1; -. [Q9P016-1]
DR CCDS; CCDS8497.1; -. [Q9P016-2]
DR RefSeq; NP_001032381.1; NM_001037304.1. [Q9P016-2]
DR RefSeq; NP_001032382.1; NM_001037305.1. [Q9P016-1]
DR RefSeq; NP_054893.1; NM_014174.2. [Q9P016-1]
DR RefSeq; NP_954994.1; NM_199297.1. [Q9P016-2]
DR RefSeq; NP_954995.1; NM_199298.1. [Q9P016-1]
DR PDB; 3EOP; X-ray; 2.30 A; A/B=55-221.
DR PDB; 5J3E; X-ray; 2.60 A; A/B=1-225.
DR PDBsum; 3EOP; -.
DR PDBsum; 5J3E; -.
DR AlphaFoldDB; Q9P016; -.
DR BMRB; Q9P016; -.
DR SMR; Q9P016; -.
DR BioGRID; 118856; 26.
DR IntAct; Q9P016; 9.
DR MINT; Q9P016; -.
DR STRING; 9606.ENSP00000341657; -.
DR GlyGen; Q9P016; 1 site, 2 O-linked glycans (1 site).
DR iPTMnet; Q9P016; -.
DR PhosphoSitePlus; Q9P016; -.
DR SwissPalm; Q9P016; -.
DR BioMuta; THYN1; -.
DR DMDM; 74734762; -.
DR EPD; Q9P016; -.
DR jPOST; Q9P016; -.
DR MassIVE; Q9P016; -.
DR MaxQB; Q9P016; -.
DR PaxDb; Q9P016; -.
DR PeptideAtlas; Q9P016; -.
DR PRIDE; Q9P016; -.
DR ProteomicsDB; 83534; -. [Q9P016-1]
DR ProteomicsDB; 83535; -. [Q9P016-2]
DR Antibodypedia; 33170; 162 antibodies from 25 providers.
DR DNASU; 29087; -.
DR Ensembl; ENST00000341541.8; ENSP00000341657.3; ENSG00000151500.15. [Q9P016-1]
DR Ensembl; ENST00000352327.5; ENSP00000341452.5; ENSG00000151500.15. [Q9P016-2]
DR Ensembl; ENST00000392594.7; ENSP00000376373.3; ENSG00000151500.15. [Q9P016-1]
DR Ensembl; ENST00000392595.6; ENSP00000376374.2; ENSG00000151500.15. [Q9P016-1]
DR GeneID; 29087; -.
DR KEGG; hsa:29087; -.
DR MANE-Select; ENST00000341541.8; ENSP00000341657.3; NM_014174.3; NP_054893.1.
DR UCSC; uc001qhf.4; human. [Q9P016-1]
DR CTD; 29087; -.
DR DisGeNET; 29087; -.
DR GeneCards; THYN1; -.
DR HGNC; HGNC:29560; THYN1.
DR HPA; ENSG00000151500; Low tissue specificity.
DR MIM; 613739; gene.
DR neXtProt; NX_Q9P016; -.
DR OpenTargets; ENSG00000151500; -.
DR PharmGKB; PA128394653; -.
DR VEuPathDB; HostDB:ENSG00000151500; -.
DR eggNOG; KOG3383; Eukaryota.
DR GeneTree; ENSGT00390000013297; -.
DR HOGENOM; CLU_041799_2_0_1; -.
DR InParanoid; Q9P016; -.
DR OMA; DVQFIRM; -.
DR OrthoDB; 1522853at2759; -.
DR PhylomeDB; Q9P016; -.
DR TreeFam; TF332126; -.
DR PathwayCommons; Q9P016; -.
DR SignaLink; Q9P016; -.
DR BioGRID-ORCS; 29087; 8 hits in 1078 CRISPR screens.
DR ChiTaRS; THYN1; human.
DR EvolutionaryTrace; Q9P016; -.
DR GeneWiki; THYN1; -.
DR GenomeRNAi; 29087; -.
DR Pharos; Q9P016; Tbio.
DR PRO; PR:Q9P016; -.
DR Proteomes; UP000005640; Chromosome 11.
DR RNAct; Q9P016; protein.
DR Bgee; ENSG00000151500; Expressed in oocyte and 208 other tissues.
DR ExpressionAtlas; Q9P016; baseline and differential.
DR Genevisible; Q9P016; HS.
DR GO; GO:0005634; C:nucleus; HDA:UniProtKB.
DR InterPro; IPR002740; EVE_domain.
DR InterPro; IPR015947; PUA-like_sf.
DR Pfam; PF01878; EVE; 1.
DR SUPFAM; SSF88697; SSF88697; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Alternative splicing; Nucleus; Phosphoprotein;
KW Reference proteome.
FT CHAIN 1..225
FT /note="Thymocyte nuclear protein 1"
FT /id="PRO_0000262564"
FT REGION 1..47
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 5..10
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000250"
FT COMPBIAS 32..47
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 161..225
FT /note="VDVQFVRMMKRFIPLAELKSYHQAHKATGGPLKNMVLFTRQRLSIQPLTQEE
FT FDFVLSLEEKEPS -> KSLILF (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334, ECO:0000303|Ref.3"
FT /id="VSP_021789"
FT CONFLICT 29
FT /note="G -> V (in Ref. 5; AAH93074)"
FT /evidence="ECO:0000305"
FT CONFLICT 223
FT /note="E -> G (in Ref. 2; AAG43118)"
FT /evidence="ECO:0000305"
FT STRAND 56..64
FT /evidence="ECO:0007829|PDB:3EOP"
FT STRAND 67..72
FT /evidence="ECO:0007829|PDB:3EOP"
FT HELIX 78..81
FT /evidence="ECO:0007829|PDB:3EOP"
FT HELIX 84..86
FT /evidence="ECO:0007829|PDB:3EOP"
FT STRAND 87..89
FT /evidence="ECO:0007829|PDB:3EOP"
FT HELIX 96..104
FT /evidence="ECO:0007829|PDB:3EOP"
FT STRAND 110..115
FT /evidence="ECO:0007829|PDB:3EOP"
FT STRAND 118..120
FT /evidence="ECO:0007829|PDB:3EOP"
FT STRAND 122..135
FT /evidence="ECO:0007829|PDB:3EOP"
FT HELIX 137..139
FT /evidence="ECO:0007829|PDB:3EOP"
FT STRAND 159..174
FT /evidence="ECO:0007829|PDB:3EOP"
FT HELIX 175..188
FT /evidence="ECO:0007829|PDB:3EOP"
FT TURN 191..194
FT /evidence="ECO:0007829|PDB:5J3E"
FT HELIX 196..199
FT /evidence="ECO:0007829|PDB:3EOP"
FT STRAND 204..208
FT /evidence="ECO:0007829|PDB:3EOP"
FT HELIX 210..218
FT /evidence="ECO:0007829|PDB:3EOP"
FT HELIX 219..221
FT /evidence="ECO:0007829|PDB:3EOP"
SQ SEQUENCE 225 AA; 25697 MW; 76E6034A6404C962 CRC64;
MSRPRKRLAG TSGSDKGLSG KRTKTENSGE ALAKVEDSNP QKTSATKNCL KNLSSHWLMK
SEPESRLEKG VDVKFSIEDL KAQPKQTTCW DGVRNYQARN FLRAMKLGEE AFFYHSNCKE
PGIAGLMKIV KEAYPDHTQF EKNNPHYDPS SKEDNPKWSM VDVQFVRMMK RFIPLAELKS
YHQAHKATGG PLKNMVLFTR QRLSIQPLTQ EEFDFVLSLE EKEPS
