ID   BRXB_ECOHS              Reviewed;         200 AA.
AC   P0DUF7; A0A7M3S2P2;
DT   02-JUN-2021, integrated into UniProtKB/Swiss-Prot.
DT   02-JUN-2021, sequence version 1.
DT   25-MAY-2022, entry version 3.
DE   RecName: Full=BREX protein BrxB {ECO:0000303|PubMed:30418590};
GN   Name=brxB {ECO:0000303|PubMed:30418590}; OrderedLocusNames=EcHS_A0337;
OS   Escherichia coli O9:H4 (strain HS).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=331112;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=HS;
RX   PubMed=18676672; DOI=10.1128/jb.00619-08;
RA   Rasko D.A., Rosovitz M.J., Myers G.S.A., Mongodin E.F., Fricke W.F.,
RA   Gajer P., Crabtree J., Sebaihia M., Thomson N.R., Chaudhuri R.,
RA   Henderson I.R., Sperandio V., Ravel J.;
RT   "The pangenome structure of Escherichia coli: comparative genomic analysis
RT   of E. coli commensal and pathogenic isolates.";
RL   J. Bacteriol. 190:6881-6893(2008).
RN   [2]
RP   FUNCTION IN ANTIVIRAL DEFENSE, INDUCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=HS;
RX   PubMed=30418590; DOI=10.1093/nar/gky1125;
RA   Gordeeva J., Morozova N., Sierro N., Isaev A., Sinkunas T., Tsvetkova K.,
RA   Matlashov M., Truncaite L., Morgan R.D., Ivanov N.V., Siksnys V., Zeng L.,
RA   Severinov K.;
RT   "BREX system of Escherichia coli distinguishes self from non-self by
RT   methylation of a specific DNA site.";
RL   Nucleic Acids Res. 47:253-265(2019).
CC   -!- FUNCTION: BREX systems (bacteriophage exclusion) provide immunity
CC       against bacteriophage. Part of a type 1 BREX system which protects
CC       against dsDNA phage. This system allows phage adsorption but prevents
CC       phage DNA replication, without degradation of the phage DNA.
CC       Methylation of bacterial DNA by PglX guides self/non-self
CC       discrimination. When the brxA-brxB-brxC-pglX-pglZ-brxL genes are
CC       transformed into a susceptible E.coli strain (BW25113) they confer very
CC       high resistance to infection by bacteriophage VR7 and VpaE1, about 100-
CC       fold protection against lambda, T5 and T7 and no protection against RNA
CC       phage Qbeta, ssDNA phage M13 or dSDNA phage T4 and VR5. Glycosylated
CC       phage DNA is not susceptible to BREX. The BREX system does not confer
CC       resistance to lysogenic lambda phage, i.e. prophage that are integrated
CC       into the chromosomal DNA and then induced to form phage.
CC       {ECO:0000269|PubMed:30418590}.
CC   -!- INDUCTION: Transcribed at slowly increasing levels as cells progress
CC       from lag to exponential to stationary phase.
CC       {ECO:0000269|PubMed:30418590}.
CC   -!- DISRUPTION PHENOTYPE: No methylation of 5'-GGTAAG-3' in chromosomal
CC       DNA, BREX no longer confers phage resistance.
CC       {ECO:0000269|PubMed:30418590}.
CC   -!- SIMILARITY: Belongs to the BrxB family. {ECO:0000305}.
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DR   EMBL; CP000802; ABV04725.1; -; Genomic_DNA.
DR   RefSeq; WP_000566901.1; NC_009800.1.
DR   AlphaFoldDB; P0DUF7; -.
DR   KEGG; ecx:EcHS_A0337; -.
DR   OMA; DKYYRAF; -.
DR   Proteomes; UP000001123; Chromosome.
DR   GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-KW.
DR   InterPro; IPR014858; BrxB.
DR   Pfam; PF08747; DUF1788; 1.
PE   1: Evidence at protein level;
KW   Antiviral defense.
FT   CHAIN           1..200
FT                   /note="BREX protein BrxB"
FT                   /id="PRO_0000452156"
SQ   SEQUENCE   200 AA;  22845 MW;  E2DA44AA1B9B052B CRC64;
     MIDPVLEYRL SQIQSRINED RFLKNNGSGN EIGFWIFDYP AQCELQVREH LKYLLRHLEK
     DHKFACLNVF QIIIDMLNER GLFERVCQQE VKVGTETLKK QLAGPLNQKK IADFIAKKVD
     LAAQDFVILT GMGNAWPLVR GHELMSALQD VMGFTPLLMF YPGTYSGYNL SPLTDTGSQN
     YYRAFRLVPD TGPAATLNPQ