ID   BRXC_ECOHS              Reviewed;        1213 AA.
AC   P0DUF8; A0A7M3S2P3;
DT   02-JUN-2021, integrated into UniProtKB/Swiss-Prot.
DT   02-JUN-2021, sequence version 1.
DT   25-MAY-2022, entry version 3.
DE   RecName: Full=Probable ATP-binding protein BrxC {ECO:0000303|PubMed:30418590};
DE   AltName: Full=BREX protein BrxC {ECO:0000303|PubMed:30418590};
GN   Name=brxC {ECO:0000303|PubMed:30418590}; OrderedLocusNames=EcHS_A0338;
OS   Escherichia coli O9:H4 (strain HS).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=331112;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=HS;
RX   PubMed=18676672; DOI=10.1128/jb.00619-08;
RA   Rasko D.A., Rosovitz M.J., Myers G.S.A., Mongodin E.F., Fricke W.F.,
RA   Gajer P., Crabtree J., Sebaihia M., Thomson N.R., Chaudhuri R.,
RA   Henderson I.R., Sperandio V., Ravel J.;
RT   "The pangenome structure of Escherichia coli: comparative genomic analysis
RT   of E. coli commensal and pathogenic isolates.";
RL   J. Bacteriol. 190:6881-6893(2008).
RN   [2]
RP   FUNCTION IN ANTIVIRAL DEFENSE, INDUCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=HS;
RX   PubMed=30418590; DOI=10.1093/nar/gky1125;
RA   Gordeeva J., Morozova N., Sierro N., Isaev A., Sinkunas T., Tsvetkova K.,
RA   Matlashov M., Truncaite L., Morgan R.D., Ivanov N.V., Siksnys V., Zeng L.,
RA   Severinov K.;
RT   "BREX system of Escherichia coli distinguishes self from non-self by
RT   methylation of a specific DNA site.";
RL   Nucleic Acids Res. 47:253-265(2019).
CC   -!- FUNCTION: BREX systems (bacteriophage exclusion) provide immunity
CC       against bacteriophage. Part of a type 1 BREX system which protects
CC       against dsDNA phage. This system allows phage adsorption but prevents
CC       phage DNA replication, without degradation of the phage DNA.
CC       Methylation of bacterial DNA by PglX guides self/non-self
CC       discrimination. When the brxA-brxB-brxC-pglX-pglZ-brxL genes are
CC       transformed into a susceptible E.coli strain (BW25113) they confer very
CC       high resistance to infection by bacteriophage VR7 and VpaE1, about 100-
CC       fold protection against lambda, T5 and T7 and no protection against RNA
CC       phage Qbeta, ssDNA phage M13 or dSDNA phage T4 and VR5. Glycosylated
CC       phage DNA is not susceptible to BREX. The BREX system does not confer
CC       resistance to lysogenic lambda phage, i.e. prophage that are integrated
CC       into the chromosomal DNA and then induced to form phage.
CC       {ECO:0000269|PubMed:30418590}.
CC   -!- INDUCTION: Transcribed at slowly increasing levels as cells progress
CC       from lag to exponential to stationary phase.
CC       {ECO:0000269|PubMed:30418590}.
CC   -!- DISRUPTION PHENOTYPE: No methylation of 5'-GGTAAG-3' in chromosomal
CC       DNA, BREX no longer confers phage resistance.
CC       {ECO:0000269|PubMed:30418590}.
CC   -!- SIMILARITY: Belongs to the BrxC family. {ECO:0000305}.
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DR   EMBL; CP000802; ABV04726.1; -; Genomic_DNA.
DR   RefSeq; WP_001019654.1; NC_009800.1.
DR   AlphaFoldDB; P0DUF8; -.
DR   KEGG; ecx:EcHS_A0338; -.
DR   OMA; NAFEGKH; -.
DR   Proteomes; UP000001123; Chromosome.
DR   GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-KW.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   SUPFAM; SSF52540; SSF52540; 1.
PE   1: Evidence at protein level;
KW   Antiviral defense.
FT   CHAIN           1..1213
FT                   /note="Probable ATP-binding protein BrxC"
FT                   /id="PRO_0000452158"
SQ   SEQUENCE   1213 AA;  139272 MW;  D38EA50CD5612374 CRC64;
     MNIEQIFKKP LKRNINGVVK AEQTDDASAY IELDEYVITR ELENHLRHFF ESYVPATGPE
     RIRMENKIGV WVSGFFGSGK SHFIKILSYL LSNRKVTHNG TERNAYSFFE EKIKDALFLA
     DINKAVHYPT EVILFNIDSR ANVDDKEDAI LKVFLKVFNE RIGYCADFPH IAHLERELDK
     RGQYETFKAA FADINGSRWE DERDAYYFIS DDMAQALSQA TQQSLEASRQ WVEQLDKNFP
     LDINNFCQWV KEWLDDNGKN ILFMVDEVGQ FIGKNTQMML KLQTITENLG VICGGRAWVI
     VTSQADINAA IGGMSSRDGQ DFSKIQGRFS TRLQLSSSNT SEVIQKRLLV KTDEAKAALA
     KVWQEKGDIL RNQLAFDTTT TTALRPFTSE EEFVDNYPFV PWHYQILQKV FESIRTKGAA
     GKQLAMGERS QLEAFQTAAQ QISAQGLDSL VPFWRFYAAI ESFLEPAVSR TITQACQNGI
     LDEFDGNLLK TLFLIRYVET LKSTLDNLVT LSIDRIDADK VELRRRVEKS LNTLERLMLI
     ARVEDKYVFL TNEEKEIENE IRNVDVDFSA INKKLASIIF DDILKSRKYR YPANKQDFDI
     SRFLNGHPLD GAVLNDLVVK ILTPKDPTYS FYNSDATCRP YTSEGDGCIL IRLPEEGRTW
     SDIDLVVQTE KFLKDNAGQR PEQATLLSEK ARENSNREKL LRVQLESLLA EADVWAIGER
     LPKKSSTPSN IVDEACRYVI ENTFGKLKML RPFNGDISRE IHALLTVEND TELDLGNLEE
     SNPDAMREVE TWISMNIEYN KPVYLRDILN HFARRPYGWP EDEVKLLVAR LACKGKFSFS
     QQNNNVERKQ AWELFNNSRR HSELRLHKVR RHDEAQVRKA AQTMADIAQQ PFNEREEPAL
     VEHIRQVFEE WKQELNVFRA KAEGGNNPGK NEIESGLRLL NAILNEKEDF ALIEKVSSLK
     DELLDFSEDR EDLVDFYRKQ FATWQKLGAA LNGSFKSNRS ALEKDAAAVK ALGELESIWQ
     MPEPYKHLNR ITLLIEQVQN VNHQLVEQHR QHALERIDAR IEESRQRLLE AHATSELQNS
     VLLPMQKARK RAEVSQSIPE ILAEQQETKA LQMDADKKIN LWIDELRKKQ EAQLRAANEA
     KRAAESEQTY VVVEKPVIQP VPKKTHLVNV ASEMRNATGG EVLETTEQVE KALDTLRTTL
     LAAIKAGDRI RLQ