TI10B_ARATH
ID TI10B_ARATH Reviewed; 249 AA.
AC Q9S7M2; Q8LBU1;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 25-MAY-2022, entry version 124.
DE RecName: Full=Protein TIFY 10B {ECO:0000303|PubMed:17499004};
DE AltName: Full=Jasmonate ZIM domain-containing protein 2 {ECO:0000303|PubMed:17637675, ECO:0000303|PubMed:19151223};
GN Name=TIFY10B {ECO:0000303|PubMed:17499004};
GN Synonyms=JAZ2 {ECO:0000303|PubMed:17637675, ECO:0000303|PubMed:19151223};
GN OrderedLocusNames=At1g74950 {ECO:0000312|Araport:AT1G74950};
GN ORFNames=F25A4.8 {ECO:0000312|EMBL:AAD55281.1},
GN F9E10.20 {ECO:0000312|EMBL:AAG51928.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP INDUCTION BY JASMONATE.
RX PubMed=17637677; DOI=10.1038/nature05960;
RA Thines B., Katsir L., Melotto M., Niu Y., Mandaokar A., Liu G., Nomura K.,
RA He S.Y., Howe G.A., Browse J.;
RT "JAZ repressor proteins are targets of the SCF(COI1) complex during
RT jasmonate signalling.";
RL Nature 448:661-665(2007).
RN [6]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=17637675; DOI=10.1038/nature06006;
RA Chini A., Fonseca S., Fernandez G., Adie B., Chico J.M., Lorenzo O.,
RA Garcia-Casado G., Lopez-Vidriero I., Lozano F.M., Ponce M.R., Micol J.L.,
RA Solano R.;
RT "The JAZ family of repressors is the missing link in jasmonate
RT signalling.";
RL Nature 448:666-671(2007).
RN [7]
RP DOMAIN.
RX PubMed=17675405; DOI=10.1105/tpc.107.050708;
RA Yan Y., Stolz S., Chetelat A., Reymond P., Pagni M., Dubugnon L.,
RA Farmer E.E.;
RT "A downstream mediator in the growth repression limb of the jasmonate
RT pathway.";
RL Plant Cell 19:2470-2483(2007).
RN [8]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=17499004; DOI=10.1016/j.tplants.2007.04.004;
RA Vanholme B., Grunewald W., Bateman A., Kohchi T., Gheysen G.;
RT "The tify family previously known as ZIM.";
RL Trends Plant Sci. 12:239-244(2007).
RN [9]
RP INTERACTION WITH COI1.
RX PubMed=18547396; DOI=10.1111/j.1365-313x.2008.03566.x;
RA Melotto M., Mecey C., Niu Y., Chung H.S., Katsir L., Yao J., Zeng W.,
RA Thines B., Staswick P., Browse J., Howe G.A., He S.Y.;
RT "A critical role of two positively charged amino acids in the Jas motif of
RT Arabidopsis JAZ proteins in mediating coronatine- and jasmonoyl isoleucine-
RT dependent interactions with the COI1 F-box protein.";
RL Plant J. 55:979-988(2008).
RN [10]
RP INDUCTION BY WOUNDING AND HERBIVORY.
RX PubMed=18223147; DOI=10.1104/pp.107.115691;
RA Chung H.S., Koo A.J., Gao X., Jayanty S., Thines B., Jones A.D., Howe G.A.;
RT "Regulation and function of Arabidopsis JASMONATE ZIM-domain genes in
RT response to wounding and herbivory.";
RL Plant Physiol. 146:952-964(2008).
RN [11]
RP FUNCTION, INTERACTION WITH TIFY10A/JAZ1; TIFY6B/JAZ3; TIFY11A/JAZ5;
RP TIFY11B/JAZ6; TIFY5A/JAZ8; TIFY7/JAZ9; TIFY9/JAZ10; TIFY3A/JAZ11 AND
RP TIFY3B/JAZ12, AND SUBUNIT.
RX PubMed=19151223; DOI=10.1105/tpc.108.064097;
RA Chung H.S., Howe G.A.;
RT "A critical role for the TIFY motif in repression of jasmonate signaling by
RT a stabilized splice variant of the JASMONATE ZIM-domain protein JAZ10 in
RT Arabidopsis.";
RL Plant Cell 21:131-145(2009).
RN [12]
RP INTERACTION WITH MYC2.
RX PubMed=19309455; DOI=10.1111/j.1365-313x.2009.03852.x;
RA Chini A., Fonseca S., Chico J.M., Fernandez-Calvo P., Solano R.;
RT "The ZIM domain mediates homo- and heteromeric interactions between
RT Arabidopsis JAZ proteins.";
RL Plant J. 59:77-87(2009).
RN [13]
RP INTERACTION WITH AFPH2/NINJA.
RX PubMed=20360743; DOI=10.1038/nature08854;
RA Pauwels L., Barbero G.F., Geerinck J., Tilleman S., Grunewald W.,
RA Perez A.C., Chico J.M., Bossche R.V., Sewell J., Gil E., Garcia-Casado G.,
RA Witters E., Inze D., Long J.A., De Jaeger G., Solano R., Goossens A.;
RT "NINJA connects the co-repressor TOPLESS to jasmonate signalling.";
RL Nature 464:788-791(2010).
RN [14]
RP FUNCTION, INTERACTION WITH MYC2; MYC3; MYC4 TIFY11A/JAZ5 AND AFPH2/NINJA,
RP AND SUBUNIT.
RX PubMed=21335373; DOI=10.1105/tpc.110.080788;
RA Fernandez-Calvo P., Chini A., Fernandez-Barbero G., Chico J.M.,
RA Gimenez-Ibanez S., Geerinck J., Eeckhout D., Schweizer F., Godoy M.,
RA Franco-Zorrilla J.M., Pauwels L., Witters E., Puga M.I., Paz-Ares J.,
RA Goossens A., Reymond P., De Jaeger G., Solano R.;
RT "The Arabidopsis bHLH transcription factors MYC3 and MYC4 are targets of
RT JAZ repressors and act additively with MYC2 in the activation of jasmonate
RT responses.";
RL Plant Cell 23:701-715(2011).
RN [15]
RP TISSUE SPECIFICITY.
RX PubMed=22173100; DOI=10.1093/pcp/pcr178;
RA Figueroa P., Browse J.;
RT "The Arabidopsis JAZ2 promoter contains a G-Box and thymidine-rich module
RT that are necessary and sufficient for jasmonate-dependent activation by MYC
RT transcription factors and repression by JAZ proteins.";
RL Plant Cell Physiol. 53:330-343(2012).
RN [16]
RP INTERACTION WITH PSEUDOMONAS SYRINGAE HOPZ1A (MICROBIAL INFECTION).
RC STRAIN=cv. Columbia;
RX PubMed=24204266; DOI=10.1371/journal.ppat.1003715;
RA Jiang S., Yao J., Ma K.-W., Zhou H., Song J., He S.Y., Ma W.;
RT "Bacterial effector activates jasmonate signaling by directly targeting JAZ
RT transcriptional repressors.";
RL PLoS Pathog. 9:e1003715-e1003715(2013).
RN [17]
RP FUNCTION, AND INTERACTION WITH RHD6 AND RSL1.
RX PubMed=31988260; DOI=10.1105/tpc.19.00617;
RA Han X., Zhang M., Yang M., Hu Y.;
RT "Arabidopsis JAZ proteins interact with and suppress RHD6 transcription
RT factor to regulate jasmonate-stimulated root hair development.";
RL Plant Cell 32:1049-1062(2020).
CC -!- FUNCTION: Repressor of jasmonate responses. Jasmonoyl-isoleucine (JA-
CC Ile) specifically promotes COI1-TIFY10B/JAZ2 interaction. Activated by
CC MYC2, MYC3 and MYC4 transcription factors (PubMed:19151223,
CC PubMed:21335373). Interacts with and suppresses RHD6 and RSL1
CC transcription factor activities to negatively regulate jasmonate-
CC stimulated root hair development (PubMed:31988260).
CC {ECO:0000269|PubMed:19151223, ECO:0000269|PubMed:21335373,
CC ECO:0000269|PubMed:31988260}.
CC -!- SUBUNIT: Homo- and heterodimer. Interacts with COI1, MYC2, MYC3, MYC4,
CC AFPH2/NINJA, TIFY10A/JAZ1, TIFY6B/JAZ3, TIFY11A/JAZ5, TIFY11B/JAZ6,
CC TIFY5A/JAZ8, TIFY7/JAZ9, TIFY9/JAZ10, TIFY3A/JAZ11 and TIFY3B/JAZ12
CC (PubMed:18547396, PubMed:19151223, PubMed:19309455, PubMed:20360743,
CC PubMed:21335373). Interacts with RHD6 and RSL1 (PubMed:31988260).
CC {ECO:0000269|PubMed:18547396, ECO:0000269|PubMed:19151223,
CC ECO:0000269|PubMed:19309455, ECO:0000269|PubMed:20360743,
CC ECO:0000269|PubMed:21335373, ECO:0000269|PubMed:31988260}.
CC -!- SUBUNIT: (Microbial infection) Interacts with the pathogenic
CC Pseudomonas syringae HopZ1a protein. {ECO:0000269|PubMed:24204266}.
CC -!- INTERACTION:
CC Q9S7M2; Q9SV55: AFPH2; NbExp=6; IntAct=EBI-1792563, EBI-1787005;
CC Q9S7M2; Q9LNJ5: BHLH13; NbExp=6; IntAct=EBI-1792563, EBI-4434261;
CC Q9S7M2; Q9ZVC9: FRS3; NbExp=6; IntAct=EBI-1792563, EBI-4448729;
CC Q9S7M2; Q39204: MYC2; NbExp=5; IntAct=EBI-1792563, EBI-1792336;
CC Q9S7M2; O49687: MYC4; NbExp=3; IntAct=EBI-1792563, EBI-15406909;
CC Q9S7M2; Q9C9L2: TCP15; NbExp=3; IntAct=EBI-1792563, EBI-4426144;
CC Q9S7M2; Q9MAH8: TCP3; NbExp=3; IntAct=EBI-1792563, EBI-25522447;
CC Q9S7M2; Q9LMA8: TIFY10A; NbExp=5; IntAct=EBI-1792563, EBI-1388539;
CC Q9S7M2; Q9S7M2: TIFY10B; NbExp=3; IntAct=EBI-1792563, EBI-1792563;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00768}.
CC -!- TISSUE SPECIFICITY: Expressed in cotyledons, hypocotyls, roots, sepals,
CC petal vascular tissue and stigmas of developing flowers. Expressed in
CC stamen filaments after jasmonic acid treatment.
CC {ECO:0000269|PubMed:22173100}.
CC -!- INDUCTION: (Microbial infection) Triggered to degradation by the
CC pathogenic Pseudomonas syringae HopZ1a protein in a COI1-dependent
CC manner, thereby activating host jasmonate signaling.
CC {ECO:0000250|UniProtKB:Q9LMA8}.
CC -!- INDUCTION: Up-regulated by jasmonate, wounding and herbivory.
CC {ECO:0000269|PubMed:17637677, ECO:0000269|PubMed:18223147}.
CC -!- DOMAIN: The jas domain (204-229) is required for interaction with COI1
CC and Pseudomonas syringae HopZ1a. {ECO:0000250|UniProtKB:Q7XPM8,
CC ECO:0000250|UniProtKB:Q9C9E3}.
CC -!- PTM: (Microbial infection) Acetylated by Pseudomonas syringae HopZ1a.
CC {ECO:0000250|UniProtKB:Q9C9E3}.
CC -!- PTM: Ubiquitinated. Targeted for degradation by the SCF(COI1) E3
CC ubiquitin ligase-proteasome pathway during jasmonate signaling.
CC {ECO:0000250|UniProtKB:Q7XPM8}.
CC -!- SIMILARITY: Belongs to the TIFY/JAZ family. {ECO:0000305}.
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DR EMBL; AC008263; AAD55281.1; -; Genomic_DNA.
DR EMBL; AC013258; AAG51928.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE35654.1; -; Genomic_DNA.
DR EMBL; AF344319; AAK06870.1; -; mRNA.
DR EMBL; AF386959; AAK62404.1; -; mRNA.
DR EMBL; BT006301; AAP13409.1; -; mRNA.
DR EMBL; AY086993; AAM64554.1; -; mRNA.
DR PIR; C96779; C96779.
DR RefSeq; NP_565096.1; NM_106153.4.
DR AlphaFoldDB; Q9S7M2; -.
DR SMR; Q9S7M2; -.
DR BioGRID; 29053; 31.
DR DIP; DIP-52214N; -.
DR ELM; Q9S7M2; -.
DR IntAct; Q9S7M2; 20.
DR STRING; 3702.AT1G74950.1; -.
DR PaxDb; Q9S7M2; -.
DR ProteomicsDB; 234301; -.
DR EnsemblPlants; AT1G74950.1; AT1G74950.1; AT1G74950.
DR GeneID; 843834; -.
DR Gramene; AT1G74950.1; AT1G74950.1; AT1G74950.
DR KEGG; ath:AT1G74950; -.
DR Araport; AT1G74950; -.
DR TAIR; locus:2027267; AT1G74950.
DR eggNOG; ENOG502RIU4; Eukaryota.
DR HOGENOM; CLU_051749_1_0_1; -.
DR InParanoid; Q9S7M2; -.
DR OrthoDB; 1280219at2759; -.
DR PhylomeDB; Q9S7M2; -.
DR PRO; PR:Q9S7M2; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9S7M2; baseline and differential.
DR Genevisible; Q9S7M2; AT.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR GO; GO:0031347; P:regulation of defense response; IBA:GO_Central.
DR GO; GO:2000022; P:regulation of jasmonic acid mediated signaling pathway; IBA:GO_Central.
DR GO; GO:0009753; P:response to jasmonic acid; IEP:TAIR.
DR GO; GO:0009611; P:response to wounding; IEP:TAIR.
DR InterPro; IPR018467; CCT_CS.
DR InterPro; IPR040390; TIFY/JAZ.
DR InterPro; IPR010399; Tify_dom.
DR PANTHER; PTHR33077; PTHR33077; 1.
DR Pfam; PF09425; Jas_motif; 1.
DR Pfam; PF06200; tify; 1.
DR SMART; SM00979; TIFY; 1.
DR PROSITE; PS51320; TIFY; 1.
PE 1: Evidence at protein level;
KW Acetylation; Jasmonic acid signaling pathway; Nucleus; Plant defense;
KW Reference proteome; Transcription; Transcription regulation;
KW Ubl conjugation.
FT CHAIN 1..249
FT /note="Protein TIFY 10B"
FT /id="PRO_0000300653"
FT DOMAIN 113..148
FT /note="Tify"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00650"
FT REGION 225..249
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 204..229
FT /note="Jas"
FT /evidence="ECO:0000255"
FT MOTIF 206..213
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00768"
FT COMPBIAS 230..249
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 31
FT /note="E -> D (in Ref. 4; AAM64554)"
FT /evidence="ECO:0000305"
FT CONFLICT 195
FT /note="N -> K (in Ref. 4; AAM64554)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 249 AA; 27072 MW; 7410B2A50505A543 CRC64;
MSSFSAECWD FSGRKPSFSQ TCTRLSRYLK EKGSFGDLSL GMTCKPDVNG GSRQPTMMNL
FPCEASGMDS SAGQEDIKPK TMFPRQSSFS SSSSSGTKED VQMIKETTKS VKPESQSAPL
TIFYGGRVMV FDDFSAEKAK EVIDLANKGS AKSFTCFTAE VNNNHSAYSQ KEIASSPNPV
CSPAKTAAQE PIQPNPASLA CELPIARRAS LHRFLEKRKD RITSKAPYQI DGSAEASSKP
TNPAWLSSR
