TI201_ARATH
ID TI201_ARATH Reviewed; 274 AA.
AC Q8GZ79; Q9MAU2;
DT 19-OCT-2011, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 86.
DE RecName: Full=Protein TIC 20-I, chloroplastic {ECO:0000303|PubMed:23372012};
DE AltName: Full=Translocon at the inner envelope membrane of chloroplasts 20-I {ECO:0000303|PubMed:23372012};
DE Short=AtTIC20-I {ECO:0000303|PubMed:23372012};
DE Flags: Precursor;
GN Name=TIC20-I {ECO:0000303|PubMed:23372012};
GN OrderedLocusNames=At1g04940 {ECO:0000312|Araport:AT1G04940};
GN ORFNames=F13M7.5, F13M7.7 {ECO:0000312|EMBL:AAF40467.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND DEVELOPMENTAL
RP STAGE.
RX PubMed=11910011; DOI=10.1105/tpc.010336;
RA Chen X., Smith M.D., Fitzpatrick L., Schnell D.J.;
RT "In vivo analysis of the role of atTic20 in protein import into
RT chloroplasts.";
RL Plant Cell 14:641-654(2002).
RN [5]
RP TISSUE SPECIFICITY.
RX PubMed=15033972; DOI=10.1074/jbc.m401968200;
RA Vojta A., Alavi M., Becker T., Hoermann F., Kuechler M., Soll J.,
RA Thomson R., Schleiff E.;
RT "The protein translocon of the plastid envelopes.";
RL J. Biol. Chem. 279:21401-21405(2004).
RN [6]
RP DISRUPTION PHENOTYPE.
RX PubMed=16891400; DOI=10.1105/tpc.106.044305;
RA Teng Y.S., Su Y.S., Chen L.J., Lee Y.J., Hwang I., Li H.M.;
RT "Tic21 is an essential translocon component for protein translocation
RT across the chloroplast inner envelope membrane.";
RL Plant Cell 18:2247-2257(2006).
RN [7]
RP FUNCTION, INTERACTION WITH TIC21 AND PRE-PROTEIN, AND DISRUPTION PHENOTYPE.
RX PubMed=19531596; DOI=10.1105/tpc.108.063552;
RA Kikuchi S., Oishi M., Hirabayashi Y., Lee D.W., Hwang I., Nakai M.;
RT "A 1-megadalton translocation complex containing Tic20 and Tic21 mediates
RT chloroplast protein import at the inner envelope membrane.";
RL Plant Cell 21:1781-1797(2009).
RN [8]
RP FUNCTION, DISRUPTION PHENOTYPE, AND TISSUE SPECIFICITY.
RX PubMed=21266460; DOI=10.1093/pcp/pcr010;
RA Hirabayashi Y., Kikuchi S., Oishi M., Nakai M.;
RT "In vivo studies on the roles of two closely related Arabidopsis Tic20
RT proteins, AtTic20-I and AtTic20-IV.";
RL Plant Cell Physiol. 52:469-478(2011).
RN [9]
RP SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND DISRUPTION PHENOTYPE.
RX PubMed=21395885; DOI=10.1111/j.1365-313x.2011.04551.x;
RA Kasmati A.R., Toepel M., Patel R., Murtaza G., Jarvis P.;
RT "Molecular and genetic analyses of Tic20 homologues in Arabidopsis thaliana
RT chloroplasts.";
RL Plant J. 66:877-889(2011).
RN [10]
RP REVIEW.
RX PubMed=20100520; DOI=10.1016/j.bbamcr.2010.01.015;
RA Kovacs-Bogdan E., Soll J., Bolter B.;
RT "Protein import into chloroplasts: the Tic complex and its regulation.";
RL Biochim. Biophys. Acta 1803:740-747(2010).
RN [11]
RP FUNCTION, COMPONENT OF THE 1-MD COMPLEX, SUBUNIT, IDENTIFICATION BY MASS
RP SPECTROMETRY, AND SUBCELLULAR LOCATION.
RX PubMed=23372012; DOI=10.1126/science.1229262;
RA Kikuchi S., Bedard J., Hirano M., Hirabayashi Y., Oishi M., Imai M.,
RA Takase M., Ide T., Nakai M.;
RT "Uncovering the protein translocon at the chloroplast inner envelope
RT membrane.";
RL Science 339:571-574(2013).
CC -!- FUNCTION: Involved in protein precursor import into chloroplasts. May
CC be part of an intermediate translocation complex acting as a protein-
CC conducting channel at the inner envelope. Seems to be specific for
CC photosynthesis-related pre-proteins. Partially redundant with TIC20-IV,
CC but not with TIC20-II or TIC20-V. {ECO:0000269|PubMed:11910011,
CC ECO:0000269|PubMed:19531596, ECO:0000269|PubMed:21266460,
CC ECO:0000269|PubMed:23372012}.
CC -!- SUBUNIT: Part of the Tic complex. Component of the 1-MD complex,
CC composed of TIC20-I, TIC214, TIC100 and TIC56 (PubMed:23372012).
CC Interacts with the translocating preproteins. Hydrolysis of ATP is
CC essential for the formation of this complex(PubMed:19531596,
CC PubMed:23372012). The 1-MD complex interacts with TIC21
CC (PubMed:19531596). {ECO:0000269|PubMed:19531596,
CC ECO:0000269|PubMed:23372012}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast inner membrane
CC {ECO:0000269|PubMed:11910011, ECO:0000269|PubMed:21395885,
CC ECO:0000269|PubMed:23372012}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:11910011, ECO:0000269|PubMed:21395885}.
CC -!- TISSUE SPECIFICITY: Expressed in leaves, shoots and roots. High
CC expression in mature photosynthetic tissues. Lower levels in non-
CC photosynthetic tissues and roots. {ECO:0000269|PubMed:11910011,
CC ECO:0000269|PubMed:15033972, ECO:0000269|PubMed:21266460,
CC ECO:0000269|PubMed:21395885}.
CC -!- DEVELOPMENTAL STAGE: Highly expressed during germination and early
CC development, with levels peaking at 25 days.
CC {ECO:0000269|PubMed:11910011}.
CC -!- DISRUPTION PHENOTYPE: Albino and seedling lethality. Tic20-I and tic20-
CC IV double mutant is embryo lethal. {ECO:0000269|PubMed:16891400,
CC ECO:0000269|PubMed:19531596, ECO:0000269|PubMed:21266460,
CC ECO:0000269|PubMed:21395885}.
CC -!- SIMILARITY: Belongs to the Tic20 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF40467.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC004809; AAF40467.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE27765.1; -; Genomic_DNA.
DR EMBL; AK117165; BAC41843.1; -; mRNA.
DR PIR; H86182; H86182.
DR RefSeq; NP_171986.3; NM_100372.5.
DR AlphaFoldDB; Q8GZ79; -.
DR STRING; 3702.AT1G04940.1; -.
DR TCDB; 3.A.9.1.2; the chloroplast envelope protein translocase (cept or tic-toc) family.
DR PaxDb; Q8GZ79; -.
DR PRIDE; Q8GZ79; -.
DR EnsemblPlants; AT1G04940.1; AT1G04940.1; AT1G04940.
DR GeneID; 839374; -.
DR Gramene; AT1G04940.1; AT1G04940.1; AT1G04940.
DR KEGG; ath:AT1G04940; -.
DR Araport; AT1G04940; -.
DR TAIR; locus:2010617; AT1G04940.
DR eggNOG; ENOG502QT65; Eukaryota.
DR HOGENOM; CLU_052258_2_0_1; -.
DR InParanoid; Q8GZ79; -.
DR OMA; FFRFHTI; -.
DR PhylomeDB; Q8GZ79; -.
DR PRO; PR:Q8GZ79; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q8GZ79; baseline and differential.
DR GO; GO:0009706; C:chloroplast inner membrane; IDA:TAIR.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0008320; F:protein transmembrane transporter activity; IDA:TAIR.
DR GO; GO:0045037; P:protein import into chloroplast stroma; IDA:TAIR.
DR InterPro; IPR005691; Tic20.
DR PANTHER; PTHR33510; PTHR33510; 1.
DR Pfam; PF16166; TIC20; 1.
DR TIGRFAMs; TIGR00994; 3a0901s05TIC20; 1.
PE 1: Evidence at protein level;
KW Chloroplast; Membrane; Plastid; Plastid inner membrane; Protein transport;
KW Reference proteome; Transit peptide; Transmembrane; Transmembrane helix;
KW Transport.
FT TRANSIT 1..65
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 66..274
FT /note="Protein TIC 20-I, chloroplastic"
FT /id="PRO_0000413663"
FT TRANSMEM 130..152
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 167..187
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 200..220
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 229..249
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 274 AA; 31204 MW; 5D7008A9F37F1660 CRC64;
MITGYSTPSA HVLMSSRAFK SSSYRAAAGQ TQHYLARSSL PVVKNSWGSP PSPFNELPRV
SRGVPLSYLS ASSSLLLNGE QGSLSGTLPV LPVRRKTLLT PRASKDVPSS FRFPPMTKKP
QWWWRTLACL PYLMPLHETW MYAETAYHLH PFLEDFEFLT YPFLGAIGRL PSWFLMAYFF
VAYLGIVRRK EWPHFFRFHV VMGMLLEIAL QVIGTVSKWM PLGVYWGKFG MHFWTAVAFA
YLFTVLESIR CALAGMYADI PFVCDAAYIQ IPYD
