TI204_ARATH
ID TI204_ARATH Reviewed; 284 AA.
AC Q9ZQZ9;
DT 19-OCT-2011, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1999, sequence version 1.
DT 03-AUG-2022, entry version 109.
DE RecName: Full=Protein TIC 20-IV, chloroplastic;
DE AltName: Full=Translocon at the inner envelope membrane of chloroplasts 20-IV;
DE Short=AtTIC20-IV;
DE Flags: Precursor;
GN Name=TIC20-IV; OrderedLocusNames=At4g03320; ORFNames=F4C21.25;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP TISSUE SPECIFICITY.
RX PubMed=15033972; DOI=10.1074/jbc.m401968200;
RA Vojta A., Alavi M., Becker T., Hoermann F., Kuechler M., Soll J.,
RA Thomson R., Schleiff E.;
RT "The protein translocon of the plastid envelopes.";
RL J. Biol. Chem. 279:21401-21405(2004).
RN [6]
RP FUNCTION, DISRUPTION PHENOTYPE, AND TISSUE SPECIFICITY.
RX PubMed=21266460; DOI=10.1093/pcp/pcr010;
RA Hirabayashi Y., Kikuchi S., Oishi M., Nakai M.;
RT "In vivo studies on the roles of two closely related Arabidopsis Tic20
RT proteins, AtTic20-I and AtTic20-IV.";
RL Plant Cell Physiol. 52:469-478(2011).
RN [7]
RP SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, AND
RP DISRUPTION PHENOTYPE.
RX PubMed=21395885; DOI=10.1111/j.1365-313x.2011.04551.x;
RA Kasmati A.R., Toepel M., Patel R., Murtaza G., Jarvis P.;
RT "Molecular and genetic analyses of Tic20 homologues in Arabidopsis thaliana
RT chloroplasts.";
RL Plant J. 66:877-889(2011).
RN [8]
RP REVIEW.
RX PubMed=20100520; DOI=10.1016/j.bbamcr.2010.01.015;
RA Kovacs-Bogdan E., Soll J., Bolter B.;
RT "Protein import into chloroplasts: the Tic complex and its regulation.";
RL Biochim. Biophys. Acta 1803:740-747(2010).
CC -!- FUNCTION: Involved in protein precursor import into chloroplasts.
CC Partially redundant with TIC20-I, but not with TIC20-II or TIC20-V.
CC {ECO:0000269|PubMed:21266460}.
CC -!- SUBUNIT: Part of the Tic complex.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast inner membrane
CC {ECO:0000269|PubMed:21395885}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:21395885}.
CC -!- TISSUE SPECIFICITY: Expressed in roots,seeds and non-photosynthetic
CC tissues. Low levels in leaves. {ECO:0000269|PubMed:15033972,
CC ECO:0000269|PubMed:21266460, ECO:0000269|PubMed:21395885}.
CC -!- DEVELOPMENTAL STAGE: Expressed throughout development.
CC {ECO:0000269|PubMed:21395885}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype. Tic20-I and tic20-IV double
CC mutant is embryo lethal. {ECO:0000269|PubMed:21266460,
CC ECO:0000269|PubMed:21395885}.
CC -!- SIMILARITY: Belongs to the Tic20 family. {ECO:0000305}.
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DR EMBL; AC005275; AAD14460.1; -; Genomic_DNA.
DR EMBL; AL161496; CAB77817.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE82306.1; -; Genomic_DNA.
DR EMBL; AF361633; AAK32801.1; -; mRNA.
DR EMBL; AY056069; AAL06969.1; -; mRNA.
DR EMBL; AY084543; AAM61111.1; -; mRNA.
DR PIR; B85042; B85042.
DR RefSeq; NP_192241.1; NM_116570.5.
DR AlphaFoldDB; Q9ZQZ9; -.
DR STRING; 3702.AT4G03320.1; -.
DR PaxDb; Q9ZQZ9; -.
DR PRIDE; Q9ZQZ9; -.
DR EnsemblPlants; AT4G03320.1; AT4G03320.1; AT4G03320.
DR GeneID; 827972; -.
DR Gramene; AT4G03320.1; AT4G03320.1; AT4G03320.
DR KEGG; ath:AT4G03320; -.
DR Araport; AT4G03320; -.
DR TAIR; locus:2125472; AT4G03320.
DR eggNOG; ENOG502RY2S; Eukaryota.
DR HOGENOM; CLU_052258_0_0_1; -.
DR InParanoid; Q9ZQZ9; -.
DR OMA; HYLRFHM; -.
DR OrthoDB; 1051552at2759; -.
DR PhylomeDB; Q9ZQZ9; -.
DR PRO; PR:Q9ZQZ9; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q9ZQZ9; baseline and differential.
DR Genevisible; Q9ZQZ9; AT.
DR GO; GO:0009507; C:chloroplast; IDA:TAIR.
DR GO; GO:0009706; C:chloroplast inner membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005739; C:mitochondrion; IDA:TAIR.
DR GO; GO:0045037; P:protein import into chloroplast stroma; IMP:TAIR.
DR InterPro; IPR005691; Tic20.
DR PANTHER; PTHR33510; PTHR33510; 1.
DR Pfam; PF16166; TIC20; 1.
DR TIGRFAMs; TIGR00994; 3a0901s05TIC20; 1.
PE 2: Evidence at transcript level;
KW Chloroplast; Membrane; Plastid; Plastid inner membrane; Protein transport;
KW Reference proteome; Transit peptide; Transmembrane; Transmembrane helix;
KW Transport.
FT TRANSIT 1..48
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 49..284
FT /note="Protein TIC 20-IV, chloroplastic"
FT /id="PRO_0000413665"
FT TRANSMEM 131..151
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 160..180
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 204..224
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 230..250
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 284 AA; 32520 MW; 8E806195D98C943C CRC64;
MQGLAATTTN RGSLTFLAPR NHSPISKKFV NPRVFFPNVD SSAKLRLSAS SISRRCPREI
APLSATASVD FAAAATSSNQ LFANGLPPLA PGLRRHRRPI EPARVAKDDF FKIKLPKIAE
RPEWWWRTLA CVPYLISLQI SDVGFYVQPF LEKHDAIGDM IYFIPGAINR WPTWFFMVYC
YLGYMWVVKN KELPHYLRFH MMMGMLLETA LQVIWCTSNF FPLIHFKGRF GMYYWMAIGF
TYICLLLECI RCALAGVYAQ IPFMTDAASI HTLFNLGGFQ RPLR
