ID   TI214_AETCO             Reviewed;        1774 AA.
AC   A4QJH4; A4QJG2;
DT   08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   15-MAY-2007, sequence version 1.
DT   25-MAY-2022, entry version 34.
DE   RecName: Full=Protein TIC 214 {ECO:0000250|UniProtKB:P56785};
DE   AltName: Full=Translocon at the inner envelope membrane of chloroplasts 214 {ECO:0000250|UniProtKB:P56785};
DE            Short=AtTIC214 {ECO:0000250|UniProtKB:P56785};
GN   Name=TIC214 {ECO:0000250|UniProtKB:P56785}; Synonyms=ycf1-A;
GN   and
GN   Name=TIC214 {ECO:0000250|UniProtKB:P56785}; Synonyms=ycf1-B;
OS   Aethionema cordifolium (Lebanon stonecress).
OG   Plastid; Chloroplast.
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Aethionemeae; Aethionema.
OX   NCBI_TaxID=434059;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Hosouchi T., Tsuruoka H., Kotani H.;
RT   "Sequencing analysis of Aethionema coridifolium chloroplast DNA.";
RL   Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Involved in protein precursor import into chloroplasts. May
CC       be part of an intermediate translocation complex acting as a protein-
CC       conducting channel at the inner envelope.
CC       {ECO:0000250|UniProtKB:P56785}.
CC   -!- SUBUNIT: Part of the Tic complex. {ECO:0000250|UniProtKB:P56785}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast inner membrane
CC       {ECO:0000250|UniProtKB:P56785}; Multi-pass membrane protein
CC       {ECO:0000255}.
CC   -!- MISCELLANEOUS: There is a partial copy of the N-terminus (positions 1-
CC       364) of ycf1 in the inverted repeat (BAF49817).
CC   -!- SIMILARITY: Belongs to the TIC214 family. {ECO:0000305}.
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DR   EMBL; AP009366; BAF49829.1; -; Genomic_DNA.
DR   EMBL; AP009366; BAF49817.1; -; Genomic_DNA.
DR   RefSeq; YP_001122992.1; NC_009265.1.
DR   RefSeq; YP_001123004.1; NC_009265.1.
DR   AlphaFoldDB; A4QJH4; -.
DR   PRIDE; A4QJH4; -.
DR   GeneID; 4968575; -.
DR   GeneID; 4968581; -.
DR   GO; GO:0009706; C:chloroplast inner membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR   InterPro; IPR008896; TIC214.
DR   PANTHER; PTHR33163; PTHR33163; 1.
DR   Pfam; PF05758; Ycf1; 1.
PE   3: Inferred from homology;
KW   Chloroplast; Membrane; Plastid; Plastid inner membrane; Protein transport;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..1774
FT                   /note="Protein TIC 214"
FT                   /id="PRO_0000326559"
FT   TRANSMEM        19..39
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        68..88
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        91..111
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        133..153
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        176..196
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        227..247
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REGION          254..275
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        254..268
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CONFLICT        345..389
FT                   /note="DSVLWFEKPFVTLVFDYKRWNRPNRYIKNDQIENAVRNEMSQYFF -> IHK
FT                   TNTRTDTKRYDFHLYIL (in Ref. 1; BAF49817)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   1774 AA;  212223 MW;  CA278593311985B0 CRC64;
     MMVFQSFILG NLVSLCMKII NSVVVVGLYY GFLTTFSIGP SYLFLLRARV MDEGEEGTEK
     KVSATTGFIA GQLMMFISIY YAPLHLALGR PHTITVLALP YLLFHFFWNN HKHFFDYGST
     TRNEMRNLRI QCVFLNNLIF QLFNHFILPS SMLARLVNIY MFRCNNKMLF VTSSFVGWLI
     GHILFMKWVG LVLVWIQQNH SIRSNVLIRS NKYKFLVSEL RNSMARIFSI LLFITCVYYL
     GRIPSPIFTK KLKGTSETEE RGGTKQDQEV STEEAPFPSL FSEEREDLDQ IDEIDEIRVN
     AKEQINKDDE FHIRTYYNYK KVSENLDGNK ENSNLEFLKI KKKEDSVLWF EKPFVTLVFD
     YKRWNRPNRY IKNDQIENAV RNEMSQYFFS ACQSDGKDRI SFSYPRNIST FFDMIQKKIP
     SFRREKTPSD KFSTCWSLIN EEKKENLKKE FLHRIEALDK EWSVEHILEK TTRFCHNETR
     KEYLPKIYDP FLQGISRGRI QRLVPFQIIT ETYIKNNIGR SWINKIHGIL LNINYQKFEQ
     TIEKFNRKSS AIEKKLSYFF DPQEEKLNSE EEIKIFKFLF DVVLTDSNDQ MLSKNFLDVH
     EIHKKVPRWS YKLRSDLEEL EGENEETIPM EPGIRARKAK RVVIFTDTEP HNEIYTNLKN
     NQNYDQNDEM VLIRYSQQSD FRREIIQGSM RPQRRKTVIW EFCQANMHSP FFFDKIGKFF
     FFSFDIRGLT KKILRNFMWK NGKKKLDKKY EDKSKRKEKR RLEIAEVWDS FLFAQILRSS
     LLVTQSILRK YIILPLLIII KNSVRMLLFQ IPEWSEDLKD WKREMHVKCT YNGVQLSETE
     FPRKWLTDGI QIKILFPFYL KPWHKSKFHS SQKGRLKKTK DKNDFCFLTV WGMETELPFG
     SAQKQPSFFE PFFKEFKKKM KKYKTKSLLV LRFFKERDKI FAKEIKNGIL KNFIFIKGKR
     NDLSKGNRIP LFDLREIYEL TETKNDSITS NPIIHELSVQ NRSMEWKNSS FSENKIKNLI
     DRINTIRNQI EAISKEKKKI TNSSNKTPYE SKIIESSKKK WQIVKRINTR LIRKIFYFVK
     FCLEQLSLGI VVGIMNIPRM TTQFFFESTK KILDKSIYKN EETEDKITKK KNTIYLISTI
     KKLISNKKKM SYDICSLSQA YVFYKLSQLQ VSNFSKLRAV LEYNICGTSL FVTNQIKDFF
     QKHGIFHYKL KEKTFLNSEI NPWKNWLRSH YQYNLPEIVW ARLVTEKWKN QINQNSLVLN
     KSLNKEDSYE KNKFDNYKKL NYLKADSLLN PKQKQNFKKD SIYNIFCYKS INSKEKSFDM
     PLEIIIDNFL VSSFRGKSNI RDIGEFRTRK YLEWRIIPFW FIKKVNIESA VDTKSQKIYI
     KTQVQNSEKI DKITKMGLAN QKSLFFDWMG MNEEILNYPI ANLEFLFFPE FFLFSSTYKI
     KPWVIPIKLL LFNFNEKKNL NKIITRNKNG FIPSNEKKYL RFYNLTNDEK EKQRNPQLAL
     PNQEKNIEEN YAESKIKKRQ NKKQYKSNTE VELDLFLTRY SRFQLRWNFF LNKKILNNVK
     IYCLLVRLKN PNKIAISSIE RGEMSLDILM IEKNLTFAKL MKKGILIIEP LRSSVKNDGQ
     LIIYRTIGIS LVHKNNHQIS QRDKKKIEKS ITQLKKKTVN RKKNNYDFFV PEKILSPKRR
     REFRILICSK LKKKSTRYRN SRFDKNIQNC GQVLNQTKYL DNDKTNLINL KFFLWPNFRL
     EDLACMNRYW FNTNNGNHFS MIRIHMYTRL KINS