ID   TI214_AETGR             Reviewed;        1791 AA.
AC   A4QJQ8; A4QJP6;
DT   08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   15-MAY-2007, sequence version 1.
DT   03-AUG-2022, entry version 40.
DE   RecName: Full=Protein TIC 214 {ECO:0000250|UniProtKB:P56785};
DE   AltName: Full=Translocon at the inner envelope membrane of chloroplasts 214 {ECO:0000250|UniProtKB:P56785};
DE            Short=AtTIC214 {ECO:0000250|UniProtKB:P56785};
GN   Name=TIC214 {ECO:0000250|UniProtKB:P56785}; Synonyms=ycf1-A;
GN   and
GN   Name=TIC214 {ECO:0000250|UniProtKB:P56785}; Synonyms=ycf1-B;
OS   Aethionema grandiflorum (Persian stone-cress).
OG   Plastid; Chloroplast.
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Aethionemeae; Aethionema.
OX   NCBI_TaxID=72657;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Hosouchi T., Tsuruoka H., Kotani H.;
RT   "Sequencing analysis of Aethionema grandiflorum chloroplast DNA.";
RL   Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Involved in protein precursor import into chloroplasts. May
CC       be part of an intermediate translocation complex acting as a protein-
CC       conducting channel at the inner envelope.
CC       {ECO:0000250|UniProtKB:P56785}.
CC   -!- SUBUNIT: Part of the Tic complex. {ECO:0000250|UniProtKB:P56785}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast inner membrane
CC       {ECO:0000250|UniProtKB:P56785}; Multi-pass membrane protein
CC       {ECO:0000255}.
CC   -!- MISCELLANEOUS: There is a partial copy of the N-terminus (positions 1-
CC       367) of ycf1 in the inverted repeat (BAF49901).
CC   -!- SIMILARITY: Belongs to the TIC214 family. {ECO:0000305}.
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DR   EMBL; AP009367; BAF49913.1; -; Genomic_DNA.
DR   EMBL; AP009367; BAF49901.1; -; Genomic_DNA.
DR   RefSeq; YP_001123076.1; NC_009266.1.
DR   RefSeq; YP_001123088.1; NC_009266.1.
DR   AlphaFoldDB; A4QJQ8; -.
DR   PRIDE; A4QJQ8; -.
DR   GeneID; 4962239; -.
DR   GeneID; 4962261; -.
DR   GO; GO:0009706; C:chloroplast inner membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR   InterPro; IPR008896; TIC214.
DR   PANTHER; PTHR33163; PTHR33163; 1.
DR   Pfam; PF05758; Ycf1; 1.
PE   3: Inferred from homology;
KW   Chloroplast; Membrane; Plastid; Plastid inner membrane; Protein transport;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..1791
FT                   /note="Protein TIC 214"
FT                   /id="PRO_0000326560"
FT   TRANSMEM        19..39
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        68..88
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        91..111
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        133..153
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        176..196
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        230..250
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REGION          257..278
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1498..1521
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        257..271
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CONFLICT        348..392
FT                   /note="DSVLWFEKPLVTLIFDYKRWNRPNRYIKNDQIENAVRNEMSQYFF -> IHK
FT                   TNTRTDKKRYGFHRYIW (in Ref. 1; BAF49901)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   1791 AA;  213490 MW;  6A0085D148BD1714 CRC64;
     MMVFQSFILG NLVSLCMKII NSVVVVGLYY GFLTTFSIGP SYLFLLRARV MDEGEEGTEK
     KVSATTGFIA GQLMMFISIY YAPLHLALGR PHTITVLALP YLLFHFFWNN HKHFFDYGST
     TRNEMRNLRI QCVFLNNLIF QLFNHFILPS SMLARLVNIY MFRCNNKMLF VTSSFVGWLI
     GHILFMKWVG LVLVWIQQNH SIRSNRSNVL IRSNKYKFLV SELRNSMARI FSILLFITCV
     YYLGRIPSPI LTKKLKGTSE TEERGGTKQD QEVSTEEAPF PSLFSEERED LDQIDEIDEI
     RVNAKEQINK DDEFHIRTYY NYNKISENID GNKENSNLEF LKIKKKEDSV LWFEKPLVTL
     IFDYKRWNRP NRYIKNDQIE NAVRNEMSQY FFSACQSDGK DRISFSYPRN LSTFSEMIQK
     KIPSFRREKN PSDQFSTCWS LINEEKKENL KKEFLNRIEA LDKEWSVEHI LEKTTRFCHN
     ETKKEYLPKI YDPFLHGISR GRTQRLFPFQ IITKTYLKNP IGGSWINKIH GILLNINYQK
     FEQTIEKFNR KSSAIKKNLS FFSDPQEEKY KSEEESKIFK FLFDVVIADS NDQTLIKNFM
     DFHEIHKKVP RWSYKLRSDL EELEGENEET IPLEEPGIRA RKAKRVVIFT DTEPHNEIYT
     NLKNNKNYDQ NDEMVLIRYS QQSDFRREII QGSMRPQRRK TVIWEFFQAN MHSPLFFDRI
     DKFFFFSFDI RGLTKQILRN FMRKNEKKKL DKKDAERSKR KEKGRLKIAE VWDSFFFAQI
     LRGFLLVTQS ILRKYILLPL LIIIKNSVRM LLFQIPEWSE DLKDWKREMH VKCTYNRVPL
     SETEFPRNWL TDGIQIKILF PFYLKPWHKS KFHSSQKGRL KKTKDKGKKK DFCFLTVWGM
     ETELPFGSAH KQPSFFGPIS KELKKKMKKY KTKSFQVLRF FKERDKIFLK GAKEIKNWIM
     KNFVFIKGKR NDLSKGNRIP LLGLREIDEL TETKNDSITS NPIIHELSVQ NRSMEWKNSS
     FSENKIKNSI DRINTIRNQI EAISKEKKKI TNSCNKPPYD SKIIESSKKK WQIVKSKNTR
     LIRKTFYFVK FCIEQLSLGI FGGIINIPRI ITQLLFESTK QIRDKSIYKN EETQEKINKK
     KNTIYLISTI KKFKSNKKKI SYDLCSLSQA YVFYKLSQLQ VSNFSKLRAV LEYNICVTSL
     FVKNQIKDFF QEHGIFHYKL KEKTFLNSES NQWKNWLRSH YQYNLPEIVW ARLVTEKWKN
     KINQDSLVLN QSLNKEDSYE KNQFDNYKKL NSFEADSLLN PKQNLKKDYI YNLFCYNSIN
     SKETIFDMPL DIIIDNFLVS SFRGKSNIRG IGKFRTRKFL DWRILTFWFI KKVNIESAVD
     TTSKKKNIQT QAQNSERIHK ITKTGLANQK KDFFDWMGMN EEILNYPIAN FDFLFFPEFV
     LFSSTYKIKP WVIPIKLLLF NFNEKKTVNK KITRNQNVFI PSNETKNLRF YNLTKESADQ
     GELESDNEKQ RNPELALPNQ EKNIEENYAE SKIKKPENKK QYKPNTEVEL DLFLTRYSRF
     QLRWNFFLNT KILNNIKIYC LLVRLKNPNE IAISSIERAE MNLDLLMIEK NFTFAKLMKK
     GILIIEPLRL SVKNDGQLII YRTIGISLVH KNNHQISQRE KKKIEKAITK YKKKTVNRKK
     KNSDFFAPEN LLSPKRRREF RILICSKFKT KSTRYRNSRF DKNIQNCGQV LNQKKDLDKD
     KTNLMKLKFF LWPNFRLEDL ACMNRYWFNT NNGNHFSMIR IHMYTRLKIN S