TI214_ARATH
ID TI214_ARATH Reviewed; 1786 AA.
AC P56785; Q27GI0;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 30-MAY-2000, sequence version 1.
DT 25-MAY-2022, entry version 102.
DE RecName: Full=Protein TIC 214 {ECO:0000303|PubMed:23372012};
DE AltName: Full=Translocon at the inner envelope membrane of chloroplasts 214 {ECO:0000303|PubMed:23372012};
DE Short=AtTIC214 {ECO:0000303|PubMed:23372012};
GN Name=TIC214 {ECO:0000303|PubMed:23372012}; Synonyms=ycf1-A;
GN OrderedLocusNames=AtCg01000 {ECO:0000312|Araport:ATCG01000};
GN and
GN Name=TIC214 {ECO:0000303|PubMed:23372012}; Synonyms=ycf1-B;
GN OrderedLocusNames=AtCg01130 {ECO:0000312|Araport:ATCG01130};
OS Arabidopsis thaliana (Mouse-ear cress).
OG Plastid; Chloroplast.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10574454; DOI=10.1093/dnares/6.5.283;
RA Sato S., Nakamura Y., Kaneko T., Asamizu E., Tabata S.;
RT "Complete structure of the chloroplast genome of Arabidopsis thaliana.";
RL DNA Res. 6:283-290(1999).
RN [2]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19376835; DOI=10.1104/pp.109.138677;
RA Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A.,
RA Grossmann J., Gruissem W., Baginsky S.;
RT "Large-scale Arabidopsis phosphoproteome profiling reveals novel
RT chloroplast kinase substrates and phosphorylation networks.";
RL Plant Physiol. 150:889-903(2009).
RN [3]
RP FUNCTION, COMPONENT OF THE 1-MD COMPLEX, SUBUNIT, IDENTIFICATION BY MASS
RP SPECTROMETRY, AND SUBCELLULAR LOCATION.
RX PubMed=23372012; DOI=10.1126/science.1229262;
RA Kikuchi S., Bedard J., Hirano M., Hirabayashi Y., Oishi M., Imai M.,
RA Takase M., Ide T., Nakai M.;
RT "Uncovering the protein translocon at the chloroplast inner envelope
RT membrane.";
RL Science 339:571-574(2013).
CC -!- FUNCTION: Involved in protein precursor import into chloroplasts. May
CC be part of an intermediate translocation complex acting as a protein-
CC conducting channel at the inner envelope.
CC {ECO:0000269|PubMed:23372012}.
CC -!- SUBUNIT: Part of the Tic complex. Component of the 1-MD complex,
CC composed of TIC20-I, TIC214, TIC100 and TIC56. Interacts with the
CC translocating preproteins. Hydrolysis of ATP is essential for the
CC formation of this complex (PubMed:23372012). The 1-MD complex interacts
CC with TIC21 (By similarity). {ECO:0000250|UniProtKB:Q8GZ79,
CC ECO:0000269|PubMed:23372012}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast inner membrane
CC {ECO:0000269|PubMed:23372012}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- MISCELLANEOUS: There is a partial copy of the N-terminus (positions 1-
CC 343) of ycf1 in the inverted repeat (ycf1-A, BAA84433).
CC -!- SIMILARITY: Belongs to the TIC214 family. {ECO:0000305}.
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DR EMBL; AP000423; BAA84433.1; -; Genomic_DNA.
DR EMBL; AP000423; BAA84445.1; -; Genomic_DNA.
DR RefSeq; NP_051105.1; NC_000932.1.
DR RefSeq; NP_051117.1; NC_000932.1.
DR AlphaFoldDB; P56785; -.
DR BioGRID; 29918; 2.
DR IntAct; P56785; 1.
DR STRING; 3702.ATCG01130.1; -.
DR TCDB; 3.A.9.1.2; the chloroplast envelope protein translocase (cept or tic-toc) family.
DR iPTMnet; P56785; -.
DR PaxDb; P56785; -.
DR PRIDE; P56785; -.
DR ProteomicsDB; 246489; -.
DR GeneID; 4386139; -.
DR GeneID; 844706; -.
DR KEGG; ath:ArthCp070; -.
DR KEGG; ath:Arthcp087; -.
DR Araport; ATCG01000; -.
DR Araport; ATCG01130; -.
DR eggNOG; ENOG502QSDY; Eukaryota.
DR HOGENOM; CLU_238474_0_0_1; -.
DR InParanoid; P56785; -.
DR OrthoDB; 53153at2759; -.
DR PRO; PR:P56785; -.
DR Proteomes; UP000006548; Chloroplast.
DR ExpressionAtlas; P56785; baseline and differential.
DR Genevisible; P56785; AT.
DR GO; GO:0009706; C:chloroplast inner membrane; IDA:UniProtKB.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0045037; P:protein import into chloroplast stroma; IDA:GO_Central.
DR InterPro; IPR008896; TIC214.
DR PANTHER; PTHR33163; PTHR33163; 1.
DR Pfam; PF05758; Ycf1; 1.
PE 1: Evidence at protein level;
KW Chloroplast; Coiled coil; Membrane; Plastid; Plastid inner membrane;
KW Protein transport; Reference proteome; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..1786
FT /note="Protein TIC 214"
FT /id="PRO_0000217297"
FT TRANSMEM 19..39
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TRANSMEM 68..88
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TRANSMEM 91..111
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TRANSMEM 133..153
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TRANSMEM 176..196
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TRANSMEM 227..247
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT COILED 1007..1046
FT /evidence="ECO:0000255"
SQ SEQUENCE 1786 AA; 213728 MW; CFFD2A4D776D7E5D CRC64;
MMVFQSFILG NLVSLCMKII NSVVVVGLYY GFLTTFSIGP SYLFLLRARV MDEGEEGTEK
KVSATTGFIA GQLMMFISIY YAPLHLALGR PHTITVLALP YLLFHFFWNN HKHFFDYGST
TRNEMRNLRI QCVFLNNLIF QLFNHFILPS SMLARLVNIY MFRCNNKMLF VTSSFVGWLI
GHILFMKWVG LVLVWIQQNN SIRSNVVIRS NKYKFLVSEL RNSMARIFSI LLFITCVYYL
GRIPSPIFTK KLKGTSETGG TKQDQEVSTE EAPFPSLFSE EGEDLDKIDE MEEIRVNGKD
KINKDDEFHV RTYYNYKTVS ENLYGNKENS NLEFFKIKKK EDHFLWFEKP FVTLVFDYKR
WNRPNRYIKN DKIENIVRNE MSQYFFYTCQ SDGKERISFT YPPNLSTFFE MIQKRIPSFT
KEKKTFDQVS TYWSLIHEEK RENLKKEFLN RIEALDKEWS VENILEKTTR FCYNEAKKEY
LPKIYDPFLH GISRGRIKKL PPFQIITETY RKNNLGGSWI NKIHGLLLKI NYKKFEQTIE
KFNRKSLSIE KKLSFFSEPQ QEEKINSEEE IKTFKFLFDI VRTDSNDQTL IKNFMDFPEI
NKKVPRWSYK LISELEELEG ENEENVPMEP GIRSRKAKRV VVFTDKEPHG EIYTNLKDNQ
NSDQNDEMAL IRYSQQSDFR REIIKGSMRS QRRKTVIWEF FQAKVHSPLF FDRIDKLFFF
SFDIWGLKKK IIKNFIWKKK IDKKEEEQSK REETRRIEIA ETWDSFLFAQ IIRGSLLVTQ
SILRKYIILP LLIIIKNSVR MLLFQFPEWS QDLKDWKREM HVKCTYNGVQ LSETEFPRNW
LTDGIQIKIL FPFYLKPWHK SKFQASQKAR LKKTKDKGEK NDFCFLTVWG METELPFGSA
QRKPSFFEPI SKELKKRIKK LKKKSFVVLK IFKERAPIFL KVAKETKNWI LKNFIFIKGI
SKRNLIPLFG PREIYELNEP KKDSIISNQM IHELSVQNKS LEWTNSSLSE KKIKNLIDRK
KTIRNQIEEI SKEKQNLTNS CTKLRYDSKI IESSKKIWQT FKRKNTRLIR KSIFFFKFCI
EQMSIAIFLG IINIPRITTQ LFFESTKKIL DKYIYKNEEN GEKKKNTLYF ISTIKNLISN
KKKMSYDLCS LSQAYVFYKL SQIKVSNFCK LKAVLEYNIC ITSFFVKNKI KVFFQEHGIF
HYELKNKTFL NSEVNQWKNW LRSQYQYNLP QISWARLVTQ NWKNKINKDS LVLNPSLTKE
DSYEKKKFDN YKKQKFFEAD SLLNPKHNVK KDSIYNLFCY KSIHSTEKNF DMSIGIALDN
CLVSSFLEKY NIRGMGEIRH RKYLDWRILN FWFTKKVTIE PWVDTKSKKK YINTKVQNYQ
KIDKITQTDL ANKKRNFFDW MGMNEEILNQ RITNFEFFFF PEFFLFSSTY KMKPWVIPIK
LLLLNFNENI NVNKKIIRKK KGFIPSNEKE SLRFYNLNKE EKESAGQVEL ESDKETKRNP
EAARLNQEKN IEENFAESTI KKRKNKKQYK SNTEAELDLF LTRYSRFQLR WNCFFNQKIL
NNVKVYCLLV RLNNPNEIAV SSIERGEMSL DILMIEKNFT FAKLMKKGIL IIEPVRLSVQ
NDGQLIIYRT IGISLVHKNK HKISKRYKKK SYINKKFFEK SITKYQNKTV NKKKNNYDFF
VPEKILSPKR RREFRILICF NLKKKNARDT NSRFDKNIQN LTTVLHKKKD LDLDKDKNNL
INLKSFLWPN FKLEDLACMN RYWFNTTNGN HFSMIRIRMY TRFPIP
