ID   TI214_BUXMI             Reviewed;        1859 AA.
AC   A6MM95; A6MM83;
DT   08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   24-JUL-2007, sequence version 1.
DT   25-MAY-2022, entry version 36.
DE   RecName: Full=Protein TIC 214 {ECO:0000250|UniProtKB:P56785};
DE   AltName: Full=Translocon at the inner envelope membrane of chloroplasts 214 {ECO:0000250|UniProtKB:P56785};
DE            Short=AtTIC214 {ECO:0000250|UniProtKB:P56785};
GN   Name=TIC214 {ECO:0000250|UniProtKB:P56785}; Synonyms=ycf1-A;
GN   and
GN   Name=TIC214 {ECO:0000250|UniProtKB:P56785}; Synonyms=ycf1-B;
OS   Buxus microphylla (Littleleaf boxwood) (Japanese boxwood).
OG   Plastid; Chloroplast.
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Buxales; Buxaceae; Buxus.
OX   NCBI_TaxID=153571;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=17644003; DOI=10.1016/j.ympev.2007.06.004;
RA   Hansen D.R., Dastidar S.G., Cai Z., Penaflor C., Kuehl J.V., Boore J.L.,
RA   Jansen R.K.;
RT   "Phylogenetic and evolutionary implications of complete chloroplast genome
RT   sequences of four early-diverging angiosperms: Buxus (Buxaceae),
RT   Chloranthus (Chloranthaceae), Dioscorea (Dioscoreaceae), and Illicium
RT   (Schisandraceae).";
RL   Mol. Phylogenet. Evol. 45:547-563(2007).
CC   -!- FUNCTION: Involved in protein precursor import into chloroplasts. May
CC       be part of an intermediate translocation complex acting as a protein-
CC       conducting channel at the inner envelope.
CC       {ECO:0000250|UniProtKB:P56785}.
CC   -!- SUBUNIT: Part of the Tic complex. {ECO:0000250|UniProtKB:P56785}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast inner membrane
CC       {ECO:0000250|UniProtKB:P56785}; Multi-pass membrane protein
CC       {ECO:0000255}.
CC   -!- MISCELLANEOUS: There is a partial copy of the N-terminus (positions 1-
CC       399) of ycf1 in the inverted repeat (ABQ45296).
CC   -!- SIMILARITY: Belongs to the TIC214 family. {ECO:0000305}.
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DR   EMBL; EF380351; ABQ45308.1; -; Genomic_DNA.
DR   EMBL; EF380351; ABQ45296.1; -; Genomic_DNA.
DR   RefSeq; YP_001294231.1; NC_009599.1.
DR   RefSeq; YP_001294243.1; NC_009599.1.
DR   AlphaFoldDB; A6MM95; -.
DR   GeneID; 5236828; -.
DR   GeneID; 5236887; -.
DR   GO; GO:0009706; C:chloroplast inner membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR   InterPro; IPR008896; TIC214.
DR   PANTHER; PTHR33163; PTHR33163; 1.
DR   Pfam; PF05758; Ycf1; 1.
PE   3: Inferred from homology;
KW   Chloroplast; Membrane; Plastid; Plastid inner membrane; Protein transport;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..1859
FT                   /note="Protein TIC 214"
FT                   /id="PRO_0000326563"
FT   TRANSMEM        18..38
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        64..84
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        87..107
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        124..144
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        172..192
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        221..241
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REGION          247..314
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        266..314
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CONFLICT        398..399
FT                   /note="FY -> KN (in Ref. 1; ABQ45296)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   1859 AA;  221676 MW;  CDB25EA827E67842 CRC64;
     MILKSFLLGN LLSLCMKIIN SVVVVGLYYG FLTTFSIGPS YLFLLRARVM EEGTEKKVSA
     TTGFITGQLM MFISIYYAPL HLALGRPHTI TVLALPYLLF HFFWNNHKHF FDYGSTTRNS
     MRNLSIQCVF LNNLIFQLFN HFILPSSTLA RLVNIYMFRC NNKMLFVTSS FVGWLIGHIL
     FMKWVGLVLF WIRQNHSIKS NVLIRSNKYL VSELRNSMAR IFSILLFITC VYYLGRMPAP
     IVTKKLKETS KTEERGESEE ERDVEIETTS ETKGTKQEQE RSTEEDPSPS LCSEEKEDPD
     KIDETEEIRV NGKEKTKDEF KETCYKSRPV YENYYLNGNQ ENSKLEILKD KEDKDLFWFE
     KPLVTLLFDY KRWNRPLRYI KNDRFENAVR NEMSQYLFYT CKNDGKQRIS FTYPPSLSTF
     LEMIQRKIYL CTKEKLSSEE MYNHWIYTNE QKKNNLRNEF INRIEALDKE FVSLNVLEKI
     TRLCNDETEQ ECSPKVYDPL LNGPYRGTIT NLYSSSIMNE TLIEDSIETL CLCINKIHGI
     LLTDYREFEH KINKFDRKSL SRDIGHLSTS IIEFDGESIP SFNFKALSLL TEDGRIDSED
     QAKFSKFLVK EISKRVPRWS YKLIDTLEQE ERENEEEVAE DHEIRSRKAK RVVIFNDNGQ
     NTDTHTNTSN DDQGNEVALI RYSQQSDFRR DIIKGSMRAQ RRKIVTWKLF QANVHSPLFL
     DRIDKTFFFS FDISGMMKII FRNWMVKNTE LKISDYEEEE KNKKETEKKN EKKREEKTRI
     LIAETWDSIL CAQVIRGSML VIQSIIRKYI VLPSLIIAKN ISRMLLFQFP EWSEDLKDWS
     REIHVKCTYN GVQLSETEFP KNWLIDGIQI KILFPFCLKP WHRSKLGSHH RDPMKNKRKK
     NDFCFLTVWG MEAELPFGSP RKRLSFFEPI CKELEKKIRK VKKNYFIVLK ILKERTKLFI
     KVSKEKKGWV IKRVLFIKRI IKELNKILFG LREVYDSSEN KNRKDYIISN QMIHESSIRI
     RSMDWTNSLL TEKKMKDLTD RTNTIRKKIE RIKRDKKKTF LTSEIKISPN ERSCDNKKLE
     SPKNIWQILK RRNTRLIRKW NYFIKFLIER IYIDALPYII NIPRIHGRLF LESKKKIMDK
     SIYNHEKNQE GIDETNKNTT HFISTIKRPL SNNNNKNSKI FCDLSSLSQA YVFYKLSQTQ
     VINKYNLRSV LQYHGASLFP KERIRYFCGT RGIFNSQSKH KRLRNFGMNE WKNWLRDHYQ
     YDLSQIKWSR LVLQKWRNRV NQRRTVQNKD SKKLGSYEKD QLIHYETEHD YEVYSLPSQK
     ENLKKNYRYD LLSYNYIHYE NKKGSYIHVS PLQVTKGREI PYNYNIHKPE FFYVLGGIPI
     SDYLGEDYII DTEENPDRKY FDWIILNFCL RKKLDIEAWT DIDTGDSINK NTKTGTNNYQ
     IIDKIDKKYL FYLTIHQEIN PSNQKKNFFD WMGMNEEILN RTISNLELWF FQEFVLLYNA
     YKIKPWVIPI KLLLLNFNRN KNVSENKNIN KKQKNDLWIP FTEKKSIELE NRNQEEKETR
     GQGDLGSDAQ NHGNLGYVLS NQQKDVEEDY AGSNIKKRRK KKQCTSNTEA ELNFLLKRYL
     LFQLRWDDPF NQRMINNIKV YCLLLRLINP KEISISSIQS GEMSPDIIQK DLTITELIKK
     GLLIIEPVRL SIKRDGQFIM YQTISISLVH KNKQQTNGRC REKRYVDKNY FDESIARHEK
     MVGTGDENHY ELLVPENISS PRRRRELRIR ICFNSGNKNF VDRNPVFFNE NKVKHCGRFL
     DENKHLDTDK KKLIKLKCFL WPNYQLEDLA CMNRYWFNTN NGSRFSMSRI HMYPRLKIR