ID   TI214_COFAR             Reviewed;        1874 AA.
AC   A0A393;
DT   08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   14-NOV-2006, sequence version 1.
DT   25-MAY-2022, entry version 35.
DE   RecName: Full=Protein TIC 214 {ECO:0000250|UniProtKB:P56785};
DE   AltName: Full=Translocon at the inner envelope membrane of chloroplasts 214 {ECO:0000250|UniProtKB:P56785};
DE            Short=AtTIC214 {ECO:0000250|UniProtKB:P56785};
GN   Name=TIC214 {ECO:0000250|UniProtKB:P56785}; Synonyms=ycf1;
OS   Coffea arabica (Arabian coffee).
OG   Plastid; Chloroplast.
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Gentianales; Rubiaceae; Ixoroideae; Gardenieae complex;
OC   Bertiereae - Coffeeae clade; Coffeeae; Coffea.
OX   NCBI_TaxID=13443;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=17309688; DOI=10.1111/j.1467-7652.2007.00245.x;
RA   Samson N., Bausher M.G., Lee S.-B., Jansen R.K., Daniell H.;
RT   "The complete nucleotide sequence of the coffee (Coffea arabica L.)
RT   chloroplast genome: organization and implications for biotechnology and
RT   phylogenetic relationships amongst angiosperms.";
RL   Plant Biotechnol. J. 5:339-353(2007).
CC   -!- FUNCTION: Involved in protein precursor import into chloroplasts. May
CC       be part of an intermediate translocation complex acting as a protein-
CC       conducting channel at the inner envelope.
CC       {ECO:0000250|UniProtKB:P56785}.
CC   -!- SUBUNIT: Part of the Tic complex. {ECO:0000250|UniProtKB:P56785}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast inner membrane
CC       {ECO:0000250|UniProtKB:P56785}; Multi-pass membrane protein
CC       {ECO:0000255}.
CC   -!- SIMILARITY: Belongs to the TIC214 family. {ECO:0000305}.
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DR   EMBL; EF044213; ABJ89737.1; -; Genomic_DNA.
DR   RefSeq; YP_817540.1; NC_008535.1.
DR   AlphaFoldDB; A0A393; -.
DR   PRIDE; A0A393; -.
DR   GeneID; 4421859; -.
DR   Proteomes; UP000515148; Chloroplast Pltd.
DR   GO; GO:0009706; C:chloroplast inner membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR   InterPro; IPR008896; TIC214.
DR   PANTHER; PTHR33163; PTHR33163; 1.
DR   Pfam; PF05758; Ycf1; 1.
PE   3: Inferred from homology;
KW   Chloroplast; Membrane; Plastid; Plastid inner membrane; Protein transport;
KW   Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..1874
FT                   /note="Protein TIC 214"
FT                   /id="PRO_0000326567"
FT   TRANSMEM        18..38
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        64..84
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        87..107
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        124..144
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        172..192
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        221..241
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REGION          248..310
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1567..1624
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        267..310
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1581..1600
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1874 AA;  223534 MW;  56F1C02E374F2752 CRC64;
     MIFQSFLLGN LVSLCMKIIN SVVVVGLYYG FLTTFSIGPS YLFLLRARVM EEGTEKKVSA
     TTGFITGQLM MFISIYYAPL HLALGRPHTI TVLALPYLLF HFFWNNHKHF FDYGSTTRNL
     MRNLSIQCVF LNNLIFQLFN YFILPSSMLA RLVNIYIFRC NNKMLFVTSS FVGWLIGHIL
     FMKWLGLVLV WIRQNHSIRS NKYIRSNKYL VSELRIFMAR IFSILLFITC VYYLGRIPSP
     ILTKKLKETS KTEEGVESEE EGDVEIETTS EMKGTKQEQE GSTEEDPSSS LFSEEKEDSN
     KIDETEEIRV NGKEKTKDEF HFHFTETGYN NIPFYDYEDS YLNMNGNQEN WKLKDKKAAK
     RKELFRFEKT LVTTLFDYNR WNHPFRYIKN DRFEKALRKE MSQYFFQIYE SNGKQRISFT
     YLPSLATFGE MIKRKISLYT LEKSSSNEFD KRWVYTNKQK IKNLNNEFRN RIQALDKEFL
     FLDILEARTR LCNDDTKNEY FPKMYDPFLK GAYRGRIKTI LSPSKTAIDN LRDTIGINRI
     HSILLPDINI DYQEFEQKAN LFDKKQLLTE MGNFLPLAGE FDIEQKSNLN MKGLSLFSDQ
     DQEKIDFEKE RKIFKFLLNV IRTYPIDQKI QKESVGIKEI IKKVPRWSYK LITDLEQQSG
     EHQENVPVDH QIRSRKAKRV VIFTDNKENT DPNKDNDTSD QRNEVALIRY SQQSDFRRRI
     IKGSMRAQRR KIVIWELFQA NVHSPLFLDR IKKSPRFSFD ISVLIKLLFR NWTGKGAKFE
     ILEYTDEETK KEDKKEKDKK EENKRKEQAR IEIAEAWDTI PFAQVIRGSM LISQSFFRKY
     IRLPVLIIGK NIVRMILFQF PEWSEDLEGW NREMHIKCTY NGVQLSETEF PKNWLTDGIQ
     IKILFPFCLK PWHRSKRRPS HRDLMKKKKQ KDDFCFLTVW GMETELPFGS PRKQSSFFEP
     AVKELETKII KFKRAYFLAL KVLKRKTKLL LRVSKETKKW IIESFLFLKR LIKELSKLNP
     IILFRFREVY ESNETKNEKD SIISNQIIHE SFSKINSPNW TNSSLTEKKK KDMSHRTSTI
     RNQIERIIKE KKKITPTPRI YISPNKRNWN TKRLELPKNI WQILKKRNAR LFSKFNSFIK
     IFVERIYIDI FLFIINITRL NTQLFIESIK KIMDKSINKA NKERINKKNQ NTIYFISTIK
     KSIDTINNKT NSYIFCDLSY LSQAYVFYKL SQTKVSNLYK LRSFFHYLGT SFFLKTEIKH
     SFEKQGIIQN ELSLKKLPSY GMDQWKGWLK GHYQYDLPSI RWSKLISEKW RNRVNQHHMA
     QNQNFKLNSY EKGHYKKQKD SEVYSLLNQK ENFQKNSRYD LLSYKYINYQ KMRDPSIYES
     PFQVQVNKNQ DFYYNSNTYK HNLFDMLESI PINPYLVKGN IRYMEKNTDR KYFDWKIIKF
     FLRKRVDIES WVKINTKSNQ NTKIGTNNYQ IIDKIDKKGP FYLLLPQNPK INSPSPKKLF
     LDWMGMNEEI LNRPISSLEF WFFPEFVLLY NLYKMKPWVI PSKVLLFNLN LNENVISNNK
     NVDGKQKTEC VIPSNKQIKN KNQNQKEKET PADQEDLRSD AQKQGNLGSV PPTKTKQEKD
     NEEDYAVSKI KAGKKKKQYK SKTEAELDFF LKRYLVFQLR WGDALNQRMI NNVKIYCLLL
     RLINPRKITI SSIQRREMSL DIMLIQKNLS LTELIKRGVL VIEPDRLSVK NDGQFIMYQI
     LGISVVHKNK HQTNQGYREQ TYVDKNRFDL LVPENILSTR CRRELRILIC FTSKNRNSVD
     KNSVFCTKNN SSQLLDKRKD LDKDKKELIK LKFFLWPNYR LEDLACMNRY WFDTNNGSRF
     SMLRIYMYPR LKSH