TI214_CUCSA
ID TI214_CUCSA Reviewed; 1879 AA.
AC Q4VZL0; A5J1Z1; Q2QD33; Q4VZM2;
DT 28-NOV-2006, integrated into UniProtKB/Swiss-Prot.
DT 11-SEP-2007, sequence version 2.
DT 25-MAY-2022, entry version 48.
DE RecName: Full=Protein TIC 214 {ECO:0000250|UniProtKB:P56785};
DE AltName: Full=Translocon at the inner envelope membrane of chloroplasts 214 {ECO:0000250|UniProtKB:P56785};
DE Short=AtTIC214 {ECO:0000250|UniProtKB:P56785};
GN Name=TIC214 {ECO:0000250|UniProtKB:P56785}; Synonyms=ycf1-A;
GN OrderedLocusNames=CsCp100;
GN and
GN Name=TIC214 {ECO:0000250|UniProtKB:P56785}; Synonyms=ycf1-B;
GN OrderedLocusNames=CsCp113;
OS Cucumis sativus (Cucumber).
OG Plastid; Chloroplast.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Cucurbitales; Cucurbitaceae; Benincaseae; Cucumis.
OX NCBI_TaxID=3659;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Baekmibaekdadagi;
RX PubMed=16362300; DOI=10.1007/s00299-005-0097-y;
RA Kim J.-S., Jung J.D., Lee J.-A., Park H.-W., Oh K.-H., Jeong W.J.,
RA Choi D.-W., Liu J.R., Cho K.Y.;
RT "Complete sequence and organization of the cucumber (Cucumis sativus L. cv.
RT Baekmibaekdadagi) chloroplast genome.";
RL Plant Cell Rep. 25:334-340(2006).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Borszczagowski;
RX PubMed=17607527; DOI=10.2478/s11658-007-0029-7;
RA Plader W.W., Yukawa Y., Sugiura M., Malepszy S.;
RT "The complete structure of the cucumber (Cucumis sativus L.) chloroplast
RT genome: its composition and comparative analysis.";
RL Cell. Mol. Biol. Lett. 12:584-594(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND VARIANTS.
RC STRAIN=cv. Chipper, and cv. Gy14;
RX PubMed=17546086; DOI=10.1139/g07-003;
RA Chung S.-M., Gordon V.S., Staub J.E.;
RT "Sequencing cucumber (Cucumis sativus L.) chloroplast genomes identifies
RT differences between chilling-tolerant and -susceptible cucumber lines.";
RL Genome 50:215-225(2007).
CC -!- FUNCTION: Involved in protein precursor import into chloroplasts. May
CC be part of an intermediate translocation complex acting as a protein-
CC conducting channel at the inner envelope.
CC {ECO:0000250|UniProtKB:P56785}.
CC -!- SUBUNIT: Part of the Tic complex. {ECO:0000250|UniProtKB:P56785}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast inner membrane
CC {ECO:0000250|UniProtKB:P56785}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- MISCELLANEOUS: There is a partial copy of the N-terminus (positions 1-
CC 222) of ycf1 in the inverted repeat (CsCp100, ycf1-A, CAJ00805).
CC -!- MISCELLANEOUS: Sequence shown corresponds to cv. Gy14/Chipper.
CC -!- SIMILARITY: Belongs to the TIC214 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAJ00805.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; DQ119058; AAZ94706.1; -; Genomic_DNA.
DR EMBL; AJ970307; CAJ00805.1; ALT_INIT; Genomic_DNA.
DR EMBL; AJ970307; CAJ00817.1; -; Genomic_DNA.
DR EMBL; DQ865975; ABI97471.1; -; Genomic_DNA.
DR EMBL; DQ865976; ABI98801.1; -; Genomic_DNA.
DR RefSeq; YP_247646.1; NC_007144.1.
DR RefSeq; YP_247658.1; NC_007144.1.
DR AlphaFoldDB; Q4VZL0; -.
DR STRING; 3659.XP_004174034.1; -.
DR PRIDE; Q4VZL0; -.
DR GeneID; 3429264; -.
DR GeneID; 3429356; -.
DR KEGG; csv:3429264; -.
DR KEGG; csv:3429356; -.
DR eggNOG; ENOG502QSDY; Eukaryota.
DR GO; GO:0009706; C:chloroplast inner membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR InterPro; IPR008896; TIC214.
DR PANTHER; PTHR33163; PTHR33163; 2.
DR Pfam; PF05758; Ycf1; 4.
PE 3: Inferred from homology;
KW Chloroplast; Membrane; Plastid; Plastid inner membrane; Protein transport;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..1879
FT /note="Protein TIC 214"
FT /id="PRO_0000262605"
FT TRANSMEM 18..38
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 64..84
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 87..107
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 124..144
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 172..192
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 218..238
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 245..305
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 586..702
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 267..305
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 586..690
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VARIANT 1352..1354
FT /note="YIY -> FFF (in strain: cv. Baekmibaekdadagi)"
FT VARIANT 1371..1372
FT /note="YI -> FF (in strain: cv. Baekmibaekdadagi)"
FT VARIANT 1384
FT /note="E -> K (in strain: cv. Baekmibaekdadagi)"
FT VARIANT 1387..1389
FT /note="YKY -> FKS (in strain: cv. Baekmibaekdadagi)"
FT VARIANT 1421
FT /note="R -> K (in strain: cv. Baekmibaekdadagi)"
FT VARIANT 1435
FT /note="R -> K (in strain: cv. Baekmibaekdadagi)"
FT VARIANT 1448
FT /note="R -> Q (in strain: cv. Baekmibaekdadagi)"
FT VARIANT 1451
FT /note="R -> K (in strain: cv. Baekmibaekdadagi)"
FT CONFLICT 116
FT /note="T -> I (in Ref. 2; CAJ00805)"
FT /evidence="ECO:0000305"
FT CONFLICT 205
FT /note="R -> S (in Ref. 2; CAJ00805)"
FT /evidence="ECO:0000305"
FT CONFLICT 222
FT /note="F -> L (in Ref. 2; CAJ00805)"
FT /evidence="ECO:0000305"
FT CONFLICT 657
FT /note="S -> F (in Ref. 2; CAJ00817)"
FT /evidence="ECO:0000305"
FT CONFLICT 733
FT /note="I -> M (in Ref. 2; CAJ00817)"
FT /evidence="ECO:0000305"
FT CONFLICT 747
FT /note="D -> G (in Ref. 2; CAJ00817)"
FT /evidence="ECO:0000305"
FT CONFLICT 1004
FT /note="I -> L (in Ref. 2; CAJ00817)"
FT /evidence="ECO:0000305"
FT CONFLICT 1245
FT /note="I -> T (in Ref. 2; CAJ00817)"
FT /evidence="ECO:0000305"
FT CONFLICT 1415
FT /note="L -> P (in Ref. 2; CAJ00817)"
FT /evidence="ECO:0000305"
FT CONFLICT 1422
FT /note="D -> G (in Ref. 2; CAJ00817)"
FT /evidence="ECO:0000305"
FT CONFLICT 1482
FT /note="K -> R (in Ref. 2; CAJ00817)"
FT /evidence="ECO:0000305"
FT CONFLICT 1848
FT /note="D -> N (in Ref. 2; CAJ00817)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1879 AA; 220989 MW; ADA2F6F778A74EC6 CRC64;
MILKSFILGN LVSLCMKIIN SVVVVGLYYG FLTTFSIGPS YLFLLRAWLM EEGTEKKVSA
TTGFITGQLM MFISIYYAPL HLALGRPYTI TVLALPYLLF HFFWTNPKHF FYYGSTTRNS
MRNLSIQCVF LNNLIFQLFN HFILPSSMLV RLVNISMFRC NNKMLFVTSS FVGWLIGHIL
FMKSVGLILV WIQQNNSIIS KKYIRSNKYL VSELRNSIAP IFSIILFITW VYYLGRIPSP
IPLKETSKTS ETAETEESEE ETDVEIETTS ETKGTKQEQE GSTEEDTSPS LFSEEKEDPD
KIDETEEIRI DKKEKYLFGF EKPLGIILFD YKRWNRPFRY IKNDKFDNAV RKEMSQYFFY
TCQSDGKDRI VFTYPPSVAT FFELIEKKIS LFSTEKLFYD EFDNPWKSLN EQKKRILNKI
VITRILLLDK GSPTLVGDVL QKRTQLCICN DETKKEYLPK IYDPFLSGPY RRRINLLLGF
SMLNETSRKN DSENIWTNKI HAIIRNNNNG FFDGVTIPYK ANASDQNSID LTEISKKVPR
WSYKLIKEIE QVYDITDGEL VLDYQIRSRK CKRVVILSDL NKIKKISTST PTSTPTSTPT
STSTPTSTPT STPTSTPTST STSTPASIPA STSTSIPAST STSTSTSIPA STSTSTSIPA
STSTSIPAST STSTSTSTSI PASTSTSTNE IKSKDEPKDE PMEQAIIRYA KQWDFRREII
KGSMRAQRRK MGIYQLFQGA NSYLFLDRHN FFFSDFCIYI PRLMEKLDRL RIRTNTELPI
SNNGEEKTRE DLKNEKNQES INLKEEKHIQ IAELWESIRF GQLVRGSLLI TQAFLRKYII
LPSVIIAANL TRILLLSPPH FFEDFKNWKK EIYVKCTYNA IPLAENEFPQ NWLTDGIQIK
ILFPFRLQSW HRSKKIEKKD FCFLTVFGTE TEQPFGSAEN PFLFFKSDLI ELREFIKKKK
QQLKKWRTKY FRIFLRLRKK VFRKINIAKE KWVIKPILFI KKRIREFWQR NPILLLQLKE
IYKLTEAKKE NNSIISNGMI QSSSGSIDSI ILSLRERKMK ELTDRINTML KIIEKMKKDR
QKELLNTKIN ISPNKMSYYD KIFESQKNIL KILKKRNVRF IRKSYKFLKF FVQNIYIDIF
QGIINMSRIN GQDSKITKSF FNNEANQERI DKPNQSIIIS TIKKLLSNIT NKNVKNQNLK
MFWDVSYLSQ AYLFYKLSQT KIINLSKLRP VFQYHGTSRF LKNEIQDYFV EVVGAHEILH
SDLKQKKQKN LENSGMINPW KNWLRGHYNF DLYPIRWSKL LSQKWRNRVK QDRKNKDFNQ
CDSYEKKGLI DYKKQNPFET HYSLPTKKKK KYIYDSLSYK SINYEDNKDS YIYELPVQVK
NNQESCYKYN KDKCKFFDIL TDLPIDKYLV KNNYLVKANI RDLEKTMDRK YFDWRMLHFS
IKNKGDIRVW RHNESDTNSK KNTQNGVKNF ELIQKINKKI IKKIKKKELF YLTINQDKKS
NPSNNKKQIF FDWMRMSEKK ISRYRSNSNP KWFFPKFLIL YNAYKIKPWM IPIKSLLFDL
NENEIFSKKK LIEKKNGNKA KKESVAKEGV DSVLSNHTKN LQKDSAGADV KKGIKKKQSK
KKIEGKLGVL RTGHFRFQLN WPNPEISKSL FMNLNGFSFM LRRLDLKKLS LAAIRNNEFS
LEFLLPPSDD ITFHELLEKG IFIIEPVRLS LKNDGKFIMS QTIGISLVQK NKYQLNQRYQ
EKGYPYKRSF AESIAIHQKR TENRTSNHYD LLVPENILSP RGRREFRILI CFNSGNKNDI
HRNTAFDDGN KVKTDDQVLD KRSKHLDRYQ NQLKNLKLFL WPNYRLEDLA CMNRYWFDTN
NSSRFSMLRI HMYPQLKIR
