ID   BRXL_BACCH              Reviewed;         676 AA.
AC   P0DUF5;
DT   07-APR-2021, integrated into UniProtKB/Swiss-Prot.
DT   07-APR-2021, sequence version 1.
DT   03-AUG-2022, entry version 6.
DE   RecName: Full=Lon-like protease BrxL {ECO:0000303|PubMed:25452498};
DE   AltName: Full=BREX protein BrxL {ECO:0000303|PubMed:25452498};
GN   Name=brxL {ECO:0000303|PubMed:25452498}; ORFNames=BCH308197_0968;
OS   Bacillus cereus (strain H3081.97).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
OC   Bacillus cereus group.
OX   NCBI_TaxID=451708;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=H3081.97;
RA   Dodson R.J., Durkin A.S., Rosovitz M.J., Rasko D.A., Hoffmaster A.,
RA   Ravel J., Sutton G.;
RT   "Genome sequence of Bacillus cereus H3081.97.";
RL   Submitted (SEP-2008) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   FUNCTION IN ANTIVIRAL DEFENSE, INDUCTION, AND CLASSIFICATION AND
RP   NOMENCLATURE.
RC   STRAIN=H3081.97;
RX   PubMed=25452498; DOI=10.15252/embj.201489455;
RA   Goldfarb T., Sberro H., Weinstock E., Cohen O., Doron S.,
RA   Charpak-Amikam Y., Afik S., Ofir G., Sorek R.;
RT   "BREX is a novel phage resistance system widespread in microbial genomes.";
RL   EMBO J. 34:169-183(2015).
CC   -!- FUNCTION: BREX systems (bacteriophage exclusion) provide immunity
CC       against bacteriophage. Part of a type 1 BREX system. This system allows
CC       phage adsorption but prevents phage DNA replication, without
CC       degradation of the phage DNA. Methylation of bacterial DNA by PglX
CC       probably guides self/non-self discrimination. When the brxA-brxB-brxC-
CC       pglX and pglZ-brxL operons are transformed into a susceptible
CC       B.subtilis strain (BEST7003) they confer resistance to bacteriophages
CC       SPbeta, SP16, Zeta, phi3T and SP02 and partial protection to phages
CC       SP01 and SP82G (these include lytic and temperate phage). They do not
CC       protect against phages phi105, rho10 or rho14. Additionally confers a
CC       very slight reduction in efficiency of plasmid transformation.
CC       {ECO:0000269|PubMed:25452498}.
CC   -!- INDUCTION: Part of the pglZ-brxL operon. {ECO:0000269|PubMed:25452498}.
CC   -!- SIMILARITY: Belongs to the BrxL family. {ECO:0000305}.
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DR   EMBL; ABDL02000007; EDZ57563.1; -; Genomic_DNA.
DR   AlphaFoldDB; P0DUF5; -.
DR   SMR; P0DUF5; -.
DR   GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR   GO; GO:0004176; F:ATP-dependent peptidase activity; IEA:InterPro.
DR   GO; GO:0004252; F:serine-type endopeptidase activity; IEA:InterPro.
DR   GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-KW.
DR   GO; GO:0030163; P:protein catabolic process; IEA:InterPro.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.230.10; -; 1.
DR   InterPro; IPR013473; BrxL.
DR   InterPro; IPR014061; BrxL-like.
DR   InterPro; IPR008269; Lon_proteolytic.
DR   InterPro; IPR027065; Lon_Prtase.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR020568; Ribosomal_S5_D2-typ_fold.
DR   InterPro; IPR014721; Ribosomal_S5_D2-typ_fold_subgr.
DR   PANTHER; PTHR10046; PTHR10046; 1.
DR   Pfam; PF13337; Lon_2; 1.
DR   Pfam; PF05362; Lon_C; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   SUPFAM; SSF54211; SSF54211; 1.
DR   TIGRFAMs; TIGR02653; Lon_rel_chp; 1.
DR   TIGRFAMs; TIGR02688; TIGR02688; 1.
PE   1: Evidence at protein level;
KW   Antiviral defense; Hydrolase; Protease.
FT   CHAIN           1..676
FT                   /note="Lon-like protease BrxL"
FT                   /id="PRO_0000452159"
SQ   SEQUENCE   676 AA;  76095 MW;  804BB3C27E0B18C4 CRC64;
     MEDLNIKLNA HFAGKVVRKD LTKKIKEGAN VPVYVLEYLL GMYCATDDEK SMNDGVQMVK
     KILSDNFVRP DEAEKVKSKV KELGKYTVID KIGVKLNDKK DIYEAEFSNL GLNGVPISSH
     YVKEFDKLLA GGIWCIVKME YYFDEESKGT SPFSIESVTP IQMPNMDLEE MFEQRRQFSK
     EEWIDVLIRS TGMEPTQLED TVKWHLLERM VPLVENNYNL CELGPRGTGK SHIYKEISPN
     SILVSGGQTT VANLFYNMST RKIGLVGMWD TVAFDEVAGI TFKDKDGIQI MKDYMASGSF
     ARGREEKNAS ASMVFVGNIN QSVDVLLKTS HLFDPFPEAM AYDSAFFDRM HYYLPGWEIP
     KMRPEFFTNE YGFITDYLAE FLREMRKRSF SDAIDKYFRL GNNLNQRDVI AVRKTVSGLI
     KLLYPNGEYI KEDVEEVLRY ALIGRRRVKE QLKKIGGMEF YDVNFSYIDN ESMNEEFVSV
     PEQGGGTLIP EGMNKPGHIY TVARGKTGMI GTYKLETEVV SGNGKFEKTG LNSDRDAKES
     IDTAFRFFKA NNKNISGTIS TTTKDYLMHI QDIHGVGLTG ELSLAAFIAL CSGALNKPVQ
     SQMVVLGSIS ISGTINKVEE LANVLQVCFD SGAKKILLPM VSAVDIPTVP PELFAKFQIG
     FYQSAEDAVF KALGVE