ID   TI214_EPIVI             Reviewed;        1738 AA.
AC   Q00383;
DT   01-APR-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1993, sequence version 1.
DT   25-MAY-2022, entry version 76.
DE   RecName: Full=Protein TIC 214 {ECO:0000250|UniProtKB:P56785};
DE   AltName: Full=Translocon at the inner envelope membrane of chloroplasts 214 {ECO:0000250|UniProtKB:P56785};
DE            Short=AtTIC214 {ECO:0000250|UniProtKB:P56785};
GN   Name=TIC214 {ECO:0000250|UniProtKB:P56785}; Synonyms=ycf1;
OS   Epifagus virginiana (Beechdrops) (Orobanche virginiana).
OG   Plastid; Non-photosynthetic plastid.
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Lamiales; Orobanchaceae; Orobancheae; Epifagus.
OX   NCBI_TaxID=4177;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1731088; DOI=10.1016/0022-2836(92)90718-y;
RA   Wolfe K.H., Morden C.W., Palmer J.D.;
RT   "Small single-copy region of plastid DNA in the non-photosynthetic
RT   angiosperm Epifagus virginiana contains only two genes. Differences among
RT   dicots, monocots and bryophytes in gene organization at a non-bioenergetic
RT   locus.";
RL   J. Mol. Biol. 223:95-104(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=1332054; DOI=10.1073/pnas.89.22.10648;
RA   Wolfe K.H., Morden C.W., Palmer J.D.;
RT   "Function and evolution of a minimal plastid genome from a
RT   nonphotosynthetic parasitic plant.";
RL   Proc. Natl. Acad. Sci. U.S.A. 89:10648-10652(1992).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1404416; DOI=10.1007/bf00161168;
RA   Wolfe K.H., Morden C.W., Ems S.C., Palmer J.D.;
RT   "Rapid evolution of the plastid translational apparatus in a
RT   nonphotosynthetic plant: loss or accelerated sequence evolution of tRNA and
RT   ribosomal protein genes.";
RL   J. Mol. Evol. 35:304-317(1992).
CC   -!- FUNCTION: Involved in protein precursor import into plasts. May be part
CC       of an intermediate translocation complex acting as a protein-conducting
CC       channel at the plastid membrane. {ECO:0000305}.
CC   -!- SUBUNIT: Part of the Tic complex. {ECO:0000250|UniProtKB:P56785}.
CC   -!- SUBCELLULAR LOCATION: Plastid membrane {ECO:0000305}; Multi-pass
CC       membrane protein {ECO:0000255}.
CC   -!- SIMILARITY: Belongs to the TIC214 family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; X61368; CAA43644.1; -; Genomic_DNA.
DR   EMBL; M81884; AAA65870.1; -; Genomic_DNA.
DR   PIR; S20614; S20614.
DR   RefSeq; NP_054395.1; NC_001568.1.
DR   AlphaFoldDB; Q00383; -.
DR   PRIDE; Q00383; -.
DR   GeneID; 801400; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0042170; C:plastid membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR   InterPro; IPR008896; TIC214.
DR   PANTHER; PTHR33163; PTHR33163; 3.
DR   Pfam; PF05758; Ycf1; 1.
PE   3: Inferred from homology;
KW   Membrane; Plastid; Protein transport; Transmembrane; Transmembrane helix;
KW   Transport.
FT   CHAIN           1..1738
FT                   /note="Protein TIC 214"
FT                   /id="PRO_0000217298"
FT   TRANSMEM        25..45
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        66..86
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        89..109
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        126..146
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        174..194
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        210..230
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        1059..1079
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   1738 AA;  208320 MW;  65C63F63BDC8364B CRC64;
     MIFKSFILGN LVSLCMKIIN INSVVVVGLY YGFLTTFSIG PSYLFLLRAQ VMEEGTENKV
     SATTGFITGQ LVMFISIYYA PLHLALGRPH TITVLALPYL FFNFFWNNHK HFFDYGSTPR
     NSMRNFSIQC VFLNNLILQL FNYFILQSSM LARLVNIYMF RCNNNILFLT SSFVGWLIGH
     ILLIKWLGLV LVWIRQNNYI RSNKYLVSEF INYMARIFSI LLFITCVYYL GRIPSPFFST
     KLTETLKKEQ QKVEDEDIET VNQMPPLGLE KREQEQEGSN IKDFYYFSEE MVNLNNNKID
     IDETEKKLVN GKKDELYPRL KITETGYKKI PIFEESSIIN INENPYNSRF KILNKKFEKK
     NLLIKEKLFV NLIFDKNRWN RPFRYIKNTH FEGATRNEMS QFFFNICEND GKERISFTYL
     SSLSFFLEMI KKKINSHKLE KALTNKLSNF WLYTNKKSIK NFTDEFINRI EALDKKFISY
     NILETRTRLC NDNYTKEYLS KKYDPLLNRS YQKTIYINLS TPILKKTPTI NLIDNFGINR
     IHGILLSYTD CQEFEQKIKR FYKKQLSTEI VDLLTFISKV VIELGPDSLN CKIFSDVKII
     KKFLLYLLTQ IVTNVNDPKI IKKSTRIKKI CKKVLRWSYK LITELEQQSG EYYENIPIDH
     QIRSRKSKSV VIFTTTKDNT DSNLYTNDQA DTDEVLIHYS QQSDFQRGII KGSMRSQRRK
     IFNLNLFQAN VHSLIFLDRI KKKPIFFFDI SESIKLIFRN WACIGKGESF KILKYTEKQT
     KREEKKEKNK KREKARIEIA EAWDTIPLAQ VIRSCVLLIQ STFRKYIILP SLIIAKNIIH
     ILLFQLPEWA EDFREWDKEV HIKCTYNGVP LSKTNFSINW LTEGIQIKIL FPFYLKTWNK
     SKLVSSQKNL IKKQKSNFCF LTVWGMEAKL PFDSPRKQLS LFNFINFVKF GKLNKKFIKL
     SKETIKWVKK KKKDYLISNQ IIHESLSKNP SLSWINYSFK EMDLADQTSK ILNQTERISK
     DKKQVTIKIF NILCENKINY NTKKRLEKWK ILIRKSERII IKLLFFFKFF IERIYTNFFL
     SSINILKINK EIFIKLIKKI IDKSIFNNER KQELINTKNK NPILFISKLL YNISNSKKNS
     YTFYGLDLYY VSQAYVFYKL SQVLVSNSYK FRADLQHLIN YNYKRIPPFF KPEKKDFLKT
     QGVVDLNFGD KKFKNYAINK WKFWLREHYK YSLSQKKRPI LISEKCRTVH WRNIASKKNN
     RYDLLSYKIL NSKNKTKTEF FLNKSSSQKN NKQEIFYISK NIPLKIFMRN IHIHKKNYFN
     NNIKNIVRFG TLYMEYIEKM ANRKYFDWKI INFLSKKKIY IETFIIINTN KNQNNKIIFN
     KDLYYFIISE KNISNSKKGF FYWMKMNAKI LNHPIFNLEN WFFPEFILLY NAYKIKPWFI
     KNKLLFINFT INKNSSKTIN EKKGPTGRED LGSVLILSQK KDIEESYERS EKEKRKKQYK
     NKTEAELDFF LKRCFLFQLR FSDTLNKRMI NNIKIYCLLL RLIDPRKIII SSIHKREINL
     DIIPIRKNLI LTELIKNGVL IIETIRFSRK YGQFSMYQTI GISLINKNKQ QTNKKYQDQI
     YFSKKKKFNL LFPENILSFR RRKKLRILIC LNSKNVNDID INPIFWNEKK IKKNIQVSYD
     NKNIDVYKKK LIKLKLFIWP NYRLEDLACM NRYWFYTNNG SRFNMLRVHL YPRLKFYE