ID   TI214_LEMMI             Reviewed;        1911 AA.
AC   A9L9E2;
DT   08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   05-FEB-2008, sequence version 1.
DT   25-MAY-2022, entry version 31.
DE   RecName: Full=Protein TIC 214 {ECO:0000250|UniProtKB:P56785};
DE   AltName: Full=Translocon at the inner envelope membrane of chloroplasts 214 {ECO:0000250|UniProtKB:P56785};
DE            Short=AtTIC214 {ECO:0000250|UniProtKB:P56785};
GN   Name=TIC214 {ECO:0000250|UniProtKB:P56785}; Synonyms=ycf1;
OS   Lemna minor (Common duckweed).
OG   Plastid; Chloroplast.
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Araceae; Lemnoideae; Lemna.
OX   NCBI_TaxID=4472;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=18463914; DOI=10.1007/s00239-008-9091-7;
RA   Mardanov A.V., Ravin N.V., Kuznetsov B.B., Samigullin T.H., Antonov A.S.,
RA   Kolganova T.V., Skyabin K.G.;
RT   "Complete sequence of the Duckweed (Lemna minor) chloroplast genome:
RT   structural organization and phylogenetic relationships to other
RT   angiosperms.";
RL   J. Mol. Evol. 66:555-564(2008).
CC   -!- FUNCTION: Involved in protein precursor import into chloroplasts. May
CC       be part of an intermediate translocation complex acting as a protein-
CC       conducting channel at the inner envelope.
CC       {ECO:0000250|UniProtKB:P56785}.
CC   -!- SUBUNIT: Part of the Tic complex. {ECO:0000250|UniProtKB:P56785}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast inner membrane
CC       {ECO:0000250|UniProtKB:P56785}; Multi-pass membrane protein
CC       {ECO:0000255}.
CC   -!- SIMILARITY: Belongs to the TIC214 family. {ECO:0000305}.
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DR   EMBL; DQ400350; ABD48541.1; -; Genomic_DNA.
DR   EMBL; DQ400350; ABD48554.1; -; Genomic_DNA.
DR   RefSeq; YP_001595554.1; NC_010109.1.
DR   RefSeq; YP_001595567.1; NC_010109.1.
DR   AlphaFoldDB; A9L9E2; -.
DR   PRIDE; A9L9E2; -.
DR   GeneID; 5787501; -.
DR   GeneID; 5787562; -.
DR   GO; GO:0009706; C:chloroplast inner membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR   InterPro; IPR008896; TIC214.
DR   PANTHER; PTHR33163; PTHR33163; 1.
DR   Pfam; PF05758; Ycf1; 1.
PE   3: Inferred from homology;
KW   Chloroplast; Membrane; Plastid; Plastid inner membrane; Protein transport;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..1911
FT                   /note="Protein TIC 214"
FT                   /id="PRO_0000326578"
FT   TRANSMEM        41..61
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        81..103
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        108..128
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        147..167
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        195..215
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        238..258
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REGION          265..299
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          798..817
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1599..1647
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        270..299
FT                   /note="Acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1619..1639
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1911 AA;  228110 MW;  23183BF83AF21A19 CRC64;
     MEVKKSSYNN REVATQKKGR RFVMILKFFL LGNPLSLCMK IINSVVIVGL YYGFMTTFSI
     GPSYLFLLRA WLMEEGTEKQ ISATTGFIMG QLIIFISIYY APLHLALGKP HTMTVLVLPY
     LLFNFFWNNN KNFLDYRSTT RNSIRNFNIQ CIFLNNFIFQ LFNHFILPSS TLARLVNIYM
     FRCNNKMLFV TSTFIGWLIG HIFFIKCVGL VLFWIRQNHS IQSNKYLLSE LRNSLARIFS
     ILLFITCVYY LGRMPLPIIT KKLKETSETE ESEENEEESD IEITSEPKEQ DEEEGSTEEN
     LYFGYEEKED LYKINETNKI PVNGKEKTKD EFHFKETYYK DSPVYEDSYL DRYQEQELWE
     LESKEDKNLF WFEKPLVTFL FDCKRWNRPF RYIKNDRFEN AVRNEMSQYF FYTCPSDGKQ
     RISFTYPPSL STFSEMIERK ISLYTTEKLS HEDNYWVYTN EQKKHTLSNE LINRIKTLEK
     KTGSLVLDVL EKRIRLCNDE NEKECLPKMY DPFLNGPYRG KITKLDSRSI RDDLITSTDA
     EESIEIVWIN KIHGLLPIPN DSPKKEFEYQ KNLFDGEFLS TNSGHSSTSI GEVPLEYPPS
     LNLKKLSLLA EEKRMDSEKQ AKCLQLIFDV VTTDYNDQTI RNQNNKLIFP GIEEIRKEVP
     RWSYKLTDDL EEQEEENEEE SMEDHEIRSR KAKRVVIYTD KEETTNAISN TSDSDQAEEV
     ALIRYSQQSD FRRDLIKGSM RAQRRKTVIW EMFQANVHSP LFLDQIDKTF FLSFDISEMM
     NLVFRGWVGR ESEFKISDSE EEETKEREKN KEKKEENERI TISETWDSII FAQAIRGSML
     VTQSILRKYI VLPSLIIAKN VGRMLLFQFP EWYEDLKDWN REMHVKCTYN GVQLSETEFP
     KDWLTDGIQI KILFPFRLKP WRKSKVRPHH RDTMKKKGKK NNFCFLTVWG REAELPFGSP
     RKQPSFFQPI WKELNNKIRK LEKTFSLSLV LRVSKKTRKW FLKISKEKTR WVNKIVLVIK
     RIMKELEKIN PIFLFGFGKV KVYESNENRK DSIISNKTTD ESTIRIRSTN WTKHSLIEKK
     VKDLADRTTT IRNQIEQITK DKKKIVVTPG ISISPNKTNC ADKNSELQKP IWQIFKRKST
     RLIRKFYYFL KYFIERIYSD ILLCTINSLR TNAQLFLEST KKMINKFFYH DETNQEGIDQ
     TNQNTIHFIS TMQKSLSNIS NKNSNISCDL SSLSQAYVFY TLSQVQVNNK YYLRSVFQYH
     GTHLFLKDRI KDYCGTRGLF DYKPKHKKVD KSGINEWKNW LKSHYQYNLS QTQWSRLVPQ
     KWRNRVNQRC TIQKKDSIIL DSYKKNQLIH YVKQNDYGID SWTGQKEKLK KNYKYDLLAH
     KYMNYEDGGD SYIYASPLQV NRDPKIPYNF NTQKTQKPES FYALVGIDIS DYLGEESSLY
     GEKNLDRKYF DCRILRFCFR KNIDIDIWTN MHIGTKINKN TKAGTQNYQK WDNKDIYPLT
     IHQKTKPSTS NKRKKLFDWM GMNKERLYRP ISNLESWFFP EFGLLYDAYK LKPWIIPIQF
     LLLNIHRNTN LSPNNNINGN QKKDLNLRSN KKEYLELENQ NQQEKKQKLS QRDLGSDTQQ
     DTQKQKKKED GEKNYTESTI KKRRKKKQFK SKKEAELDFF LKKYFLFQLR WDDSLNQRMI
     NNIKVYCLLL RLINLKEIAI SSIQRGEMRL DVMLIQKDLS LTELIKRGIF IIEPVRLSIK
     WDEEFFIYQT LGISLVNKSK QQSETDRRYI QKYLDENPFD KSISRHSTML VSEDKNYYDL
     FVPENILSTR RRRELRILIC FNSWKGNVVD VDRNFIFFNE NDIRNCGQFL KKDKHFNTDP
     NKNKLMKLKL FLWPNYRLED LACMNRYWFD TNNGSRFSMS RIQMYPQFRI Y