ID   TI214_LOTJA             Reviewed;        1793 AA.
AC   Q9BBN6;
DT   10-OCT-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2001, sequence version 1.
DT   25-MAY-2022, entry version 56.
DE   RecName: Full=Protein TIC 214 {ECO:0000250|UniProtKB:P56785};
DE   AltName: Full=Translocon at the inner envelope membrane of chloroplasts 214 {ECO:0000250|UniProtKB:P56785};
DE            Short=AtTIC214 {ECO:0000250|UniProtKB:P56785};
GN   Name=TIC214 {ECO:0000250|UniProtKB:P56785}; Synonyms=ycf1;
OS   Lotus japonicus (Lotus corniculatus var. japonicus).
OG   Plastid; Chloroplast.
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC   NPAAA clade; Hologalegina; robinioid clade; Loteae; Lotus.
OX   NCBI_TaxID=34305;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Miyakojima MG-20;
RX   PubMed=11214967; DOI=10.1093/dnares/7.6.323;
RA   Kato T., Kaneko T., Sato S., Nakamura Y., Tabata S.;
RT   "Complete structure of the chloroplast genome of a legume, Lotus
RT   japonicus.";
RL   DNA Res. 7:323-330(2000).
CC   -!- FUNCTION: Involved in protein precursor import into chloroplasts. May
CC       be part of an intermediate translocation complex acting as a protein-
CC       conducting channel at the inner envelope.
CC       {ECO:0000250|UniProtKB:P56785}.
CC   -!- SUBUNIT: Part of the Tic complex. {ECO:0000250|UniProtKB:P56785}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast inner membrane
CC       {ECO:0000250|UniProtKB:P56785}; Multi-pass membrane protein
CC       {ECO:0000255}.
CC   -!- SIMILARITY: Belongs to the TIC214 family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AP002983; BAB33253.1; -; Genomic_DNA.
DR   RefSeq; NP_084853.1; NC_002694.1.
DR   AlphaFoldDB; Q9BBN6; -.
DR   PRIDE; Q9BBN6; -.
DR   GeneID; 802851; -.
DR   GO; GO:0009706; C:chloroplast inner membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR   InterPro; IPR008896; TIC214.
DR   PANTHER; PTHR33163; PTHR33163; 3.
DR   Pfam; PF05758; Ycf1; 2.
PE   3: Inferred from homology;
KW   Chloroplast; Membrane; Plastid; Plastid inner membrane; Protein transport;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..1793
FT                   /note="Protein TIC 214"
FT                   /id="PRO_0000217299"
FT   TRANSMEM        11..31
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        64..84
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        90..112
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        129..149
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        172..192
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        222..242
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REGION          1504..1524
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1793 AA;  214752 MW;  E26F884C11A92096 CRC64;
     MIFQSFILDN LVSLCLKIIN SVIVVGLYYG FMTTFSTGPS YLFLLRAHVM EEGTEKKISA
     TTGFITGQLV MFISIYYAPL HIALDRPHTI TVITLPYLLL YFLGNNQKNF LNYVYKNQNS
     IRHFSIQRIF FQNLFFQLLN PFFLPSSILM RLANIYIFQS NNKVLFLTSS FVGWLIGHVF
     FMKWIGLMLV WIQEKNNSIK STVAIRSNKG VLAKFRKSMF QIFLIFFFIT CLYYLGRIPP
     IYFFTPKMSE IKERGEIEKR EGEIDIEINS QRAGSKQEQK ITAEEKLSPY LFSKKNNNLD
     KIKEENDIFG FQKPLVTILF DYNRWNRPLR YIKNDRFENV VRNEISQFFF FTCQSDGKER
     ISFTYPPNLS TFQKMMEMKI SLFTRDIISY EELSNSWRST NEEKKKKLTN EFLNRVEVLD
     KESLPVDIFE NRIRLCNDEK KQKYLTKEYD PFLNGPCRGQ IQKWFSPPIQ KETYKKNSLF
     INKIHGILFS NTNNYPKFEQ KKNIFDRKSL LTDINFFFNL ITKFSRKSVS SLNFEGLYLF
     PKDNKGKMSS KKKKFLFDTI RPDLNDNKIV NLQKCIGINE IVKKLPRWSY NLIDELEQLE
     GKKKVEYHQI RSRKAKRVVL LTKNSQNDDN YDETTDTDNT EKKKELALIR YSQQPDFRRD
     IIKGSIRAQR RKTVTCKLFQ RSVDSPLFLE KMEKTSFFCF DILDSSKIFF MFKNWIRKKK
     ELKNSDYTDE KAKESQKKEE EKIKKNEKEE KRRIEIGEAW DSIIFAQVIR GCLLITQSIL
     RKYILLPSLI ITKNIVRILL FQFPEWSEDF RDWQREMYIK CTYNGVQLSE TEFPKKWLTD
     GIQIKILFPF RLKPWHRSKL RFTEKKKDPL KNKKVKKKNF CFLTIFGMEV ELPFSGYPRN
     RFSFFDPILK ELKKKMKKLK NNFFLILKIV NERTKNFITT LKETSKRIIQ SILKKVLFLN
     KKIKKLYNYL FLFRFKKIDE LNQNKKNFPI TKNNPIIYES TILIQAINKT NCSLTEKKIK
     AINAKTKKII KKIERMTKEN KGGFLISEIN SNSKKTSSNT KGLELEKKIL QILQRRNVQL
     THKLYSFFKF LLNFMKKVYT DIFLCIVSVP RINVQFFLES TKKIINQSIY NKKTNEEIID
     KTNQSIIHFI SIINKSSNTK NTNSAANSYE VSALSQAYVF FKISQIQVLN VYKYKFKYVF
     DYDGRSFFIK DEIKDYFFGI QGIIHSKLRH KNSPVSLKNQ WTNWLKVHYQ YDLSQNRWSR
     LVQKNLKNRI NKHRLDQNKD LTKCDSYKKT QLIVSKNKKQ QVDFLVNLLI QKKIKKQSRY
     DLLLYKFINY AEKKELSIYG YRSPFQANKK RAISYDYNTQ KKEFFDRMDD ISIKNYIAED
     AIRYIEQNRD RKYFDWVVMD VKIQNNSISN LQFSFFFKFL RFYDAYRNKP WIIPIKFLFL
     HFSVNQNFNK IKNIIEKKRR IDIFKPWKKK KILEVELETP NRAKKEYTSR VDLNKPSLSN
     QEKDIEEDYG ESDSKKGGKD KNKKKYKNKI EAEVNLLLRK YLNFHLNWKG SLNKRVINNV
     KVYCLLIRLK NIKQIAISSI QRGELSLDIM MIQNEKDSTL TGFRKKKEFI EKGIFIIEPV
     RLSRKNNEQF FMYETARLLL IHKSKRQINQ RNPEKSDLDK QIFYKNIPPK RDQRITQNKE
     KKHYALVVIE NILSARRRRE LRILICFNPR SINSMPRKTI FDNENKINNC CQVFAKNKDL
     DKEKKILMNL KLILWPNYRL EDLACINRYW FDTYNGSRFS IVRIHMYPRL KMR