ID   TI214_NICTO             Reviewed;        1892 AA.
AC   Q33BW4; Q33BX6;
DT   28-NOV-2006, integrated into UniProtKB/Swiss-Prot.
DT   06-DEC-2005, sequence version 1.
DT   25-MAY-2022, entry version 40.
DE   RecName: Full=Protein TIC 214 {ECO:0000250|UniProtKB:P56785};
DE   AltName: Full=Translocon at the inner envelope membrane of chloroplasts 214 {ECO:0000250|UniProtKB:P56785};
DE            Short=AtTIC214 {ECO:0000250|UniProtKB:P56785};
GN   Name=TIC214 {ECO:0000250|UniProtKB:P56785}; Synonyms=ycf1-A;
GN   and
GN   Name=TIC214 {ECO:0000250|UniProtKB:P56785}; Synonyms=ycf1-B;
OS   Nicotiana tomentosiformis (Tobacco).
OG   Plastid; Chloroplast.
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC   Nicotiana.
OX   NCBI_TaxID=4098;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=16435119; DOI=10.1007/s00438-005-0092-6;
RA   Yukawa M., Tsudzuki T., Sugiura M.;
RT   "The chloroplast genome of Nicotiana sylvestris and Nicotiana
RT   tomentosiformis: complete sequencing confirms that the Nicotiana sylvestris
RT   progenitor is the maternal genome donor of Nicotiana tabacum.";
RL   Mol. Genet. Genomics 275:367-373(2006).
CC   -!- FUNCTION: Involved in protein precursor import into chloroplasts. May
CC       be part of an intermediate translocation complex acting as a protein-
CC       conducting channel at the inner envelope.
CC       {ECO:0000250|UniProtKB:P56785}.
CC   -!- SUBUNIT: Part of the Tic complex. {ECO:0000250|UniProtKB:P56785}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast inner membrane
CC       {ECO:0000250|UniProtKB:P56785}; Multi-pass membrane protein
CC       {ECO:0000255}.
CC   -!- MISCELLANEOUS: There is a partial copy of the N-terminus (positions 1-
CC       337) of ycf1 in the inverted repeat (BAE48059).
CC   -!- SIMILARITY: Belongs to the TIC214 family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAE48059.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; AB240139; BAE48071.1; -; Genomic_DNA.
DR   EMBL; AB240139; BAE48059.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; YP_398919.1; NC_007602.1.
DR   RefSeq; YP_398931.1; NC_007602.1.
DR   AlphaFoldDB; Q33BW4; -.
DR   PRIDE; Q33BW4; -.
DR   GeneID; 3776313; -.
DR   GeneID; 3776412; -.
DR   KEGG; nto:3776313; -.
DR   KEGG; nto:3776412; -.
DR   OrthoDB; 53153at2759; -.
DR   GO; GO:0009706; C:chloroplast inner membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR   InterPro; IPR008896; TIC214.
DR   PANTHER; PTHR33163; PTHR33163; 1.
DR   Pfam; PF05758; Ycf1; 1.
PE   3: Inferred from homology;
KW   Chloroplast; Membrane; Plastid; Plastid inner membrane; Protein transport;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..1892
FT                   /note="Protein TIC 214"
FT                   /id="PRO_0000262618"
FT   TRANSMEM        18..38
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        64..84
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        87..107
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        124..144
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        172..192
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        221..241
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REGION          250..300
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          794..814
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1581..1609
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        266..283
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1581..1601
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CONFLICT        337
FT                   /note="P -> E (in Ref. 1; BAE48059)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   1892 AA;  225429 MW;  F495F5E0AB703D9E CRC64;
     MIFQSFLLGN LVSLCMKIIN SVVVVGLYYG FLTTFSIGPS YLFLLRALVM EEGTEKKVSA
     TTGFITGQLM MFISIYYAPL HLALGRPHTI TVLALPYLLF HFFWNNHKHF FDYGSTTRNS
     MRNLSIQCVF LNNLIFQLFN HFILPSSMLA RLVNIYLFRC NSKILFVTSG FVGWLIGHIF
     FMKWLGLVLV WIRQNHSIRS NKYIRSNKYL VLELRNSMAR IFSILLFITC VYYLGRIPSP
     ILTKKLKEAS KTEERVESEE EKDVEIETAS EMKGTKQEQE GSTEEDPYPS PSLFSEEGWD
     PDKIDETEEI RVNGKDKIKD KFHSHLTETG YNTSNCPIYD YEDSYLNNNN TGNPENFKLQ
     LLDKKNENKE LFWFQQPLVS LLFDYNRWNR PFRYIKNNRF EQAVRTEMSQ YFFDTCKSDG
     KQRISFTYPP SLSTFWKMIK RRIPLLSLQK RLPNELDNQW ISTNKEKSTN LNKEFLNRLE
     VLDKESFSLD ILETRTRLCN DDTKKEYVPK MYDPLLNGPY RGTIKKEFSP SIINNTSLEN
     LKERVRINRI HTIFLPNTDY QEFEQKVDTV DKKPLSTEID EFLTLINEFD NEPKSSLNLK
     DLSLFSDQEQ GRVNSEKRTK FVKFVFNAID PNGTTSEKKL IGIKEISKKI PRWSHKLITE
     LEQQSGDYQE GVPLDHQIRS RKAKRVVIFT ANNQNNDPNT KDTDTADQDQ TKEVALIRYS
     QQPDFRRGII KGSMRAQRRK TVIWKLFQAN VHSPLFLERI TPPLLFSFDI SGLIKPIFRN
     WSGKEGEFKI LESREEQTKR EEKKEKDKKG ENKRKEKARI EIAEAWDTIP FAQIIRGYML
     ITQSILRKYI VLPSLIIAKN LGRMLFLQLP EWSEDLQEWN REMHIKCTYN GVQLSETEFP
     KNWLKDGIQI KILFPFCLKP WHISKLYSSR GELMKKKKQK DDFCFLTVWG MEAELPFGSP
     RKRPSFFEPI FKELEKKIGK FKKKYFITLK VLKGKIKLFR RVSKETKKWL IKSSLFIKKM
     KKELSKVNPI VLFRFKEIDE SNETKKEKDS LISNQIINEP FSQIESGNWP NSSLIESKMK
     DLTDRTSTIK NQIERITKEK KKVTPEIDIS PNKSPNKTNN IKKFESPKNI FQILKRRNTR
     LIWKFHYFLK FFIQRLYIDL FLSIINIPRI NTQLFLESTN KLIDKYISKN EINQKKIHFI
     STIKKSLYNI SKKNSHIFFD LSYLSQAYVF YKLSQTQVIN LSKLRSVLQY NRTSFFLKTK
     LKDYFRTLGI FHSELKHKKL QSYRINQWKN WLRRHYQYDL SQIRWSRLMP QKRRNRVNQS
     CMAQNRNLNK WNSYEKDQLI HYKKENDSEL YSLSNQKDNF KKCYRYDLLA YKSINYENKN
     DSFICRLPFQ VNKNLEISSN SNTSKHNLFY MLGNLHINNY LRKGNILYIE RNLDRKYFDW
     KIIHFSLRQK EDIEAWVKSD TNSNPNTKIG INNYQIIDKI EKKGLFYLTI HQNPENNQKN
     SKNDFFDWMG MNEKILNRPI LNLEFWLFPE FVPFYNVYKI KPWIIPSKLL LLNLNTNENV
     SQNKNKKQNF FLRSNKKIKN RIQEEKEPAS QGEKERGSDI ENKGNLGPVL SKHQNALKKD
     YTESDTKKGK KKKQYKSNTE AELDLFLKRY LLFQLRWNDA LNQRMIENIK VYCLLLRLIN
     PSKIAISSIQ RREMSLDIML IQKNLTLTEL MKKGILIIEP IRLSVKNNGQ FIMYQTIGIS
     LVHKSKHQTN QRYPEQRYVD KKNFDEFILQ PQTQRINTDK NHFDLLVPEN ILWSRRRREL
     RIRSFFNSLN WNGVDRNSVF CNENNVKNWS QFLDERKPLY KEKNELIKLK FFLWPNYRLE
     DLACMNRYWF DTNNGSRFSI LRIHMYPRLK IN