ID   TI214_OENBE             Reviewed;        1005 AA.
AC   P31563;
DT   01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1993, sequence version 1.
DT   25-MAY-2022, entry version 60.
DE   RecName: Full=Protein TIC 214 {ECO:0000250|UniProtKB:P56785};
DE   AltName: Full=Translocon at the inner envelope membrane of chloroplasts 214 {ECO:0000250|UniProtKB:P56785};
DE            Short=AtTIC214 {ECO:0000250|UniProtKB:P56785};
DE   Flags: Fragment;
GN   Name=TIC214 {ECO:0000250|UniProtKB:P56785}; Synonyms=ycf1;
OS   Oenothera berteroana (Bertero's evening primrose).
OG   Plastid; Chloroplast.
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Myrtales; Onagraceae; Onagroideae; Onagreae; Oenothera.
OX   NCBI_TaxID=3950;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8435856; DOI=10.1007/bf00351505;
RA   Nimzyk R., Schoendorf T., Hachtel W.;
RT   "In-frame length mutations associated with short tandem repeats are located
RT   in unassigned open reading frames of Oenothera chloroplast DNA.";
RL   Curr. Genet. 23:265-270(1993).
CC   -!- FUNCTION: Involved in protein precursor import into chloroplasts. May
CC       be part of an intermediate translocation complex acting as a protein-
CC       conducting channel at the inner envelope.
CC       {ECO:0000250|UniProtKB:P56785}.
CC   -!- SUBUNIT: Part of the Tic complex. {ECO:0000250|UniProtKB:P56785}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast inner membrane
CC       {ECO:0000250|UniProtKB:P56785}; Multi-pass membrane protein
CC       {ECO:0000255}.
CC   -!- SIMILARITY: Belongs to the TIC214 family. {ECO:0000305}.
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DR   EMBL; X64614; CAA45895.1; -; Genomic_DNA.
DR   AlphaFoldDB; P31563; -.
DR   GO; GO:0009706; C:chloroplast inner membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR   InterPro; IPR008896; TIC214.
DR   PANTHER; PTHR33163; PTHR33163; 3.
DR   Pfam; PF05758; Ycf1; 3.
PE   3: Inferred from homology;
KW   Chloroplast; Membrane; Plastid; Plastid inner membrane; Protein transport;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..>1005
FT                   /note="Protein TIC 214"
FT                   /id="PRO_0000217304"
FT   TRANSMEM        25..45
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        67..87
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        91..111
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        131..151
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        177..197
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        304..324
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REGION          457..481
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          767..833
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        462..481
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        785..807
FT                   /note="Basic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        808..822
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   NON_TER         1005
SQ   SEQUENCE   1005 AA;  117043 MW;  E868FEC7FAAA693C CRC64;
     MTLKMFLPVN LVSVCMKLNN SVVMVGLYYG FISAFSIGSS YLFLLRPRFL NDDPDAIEKK
     ASETAGFFTG QLLIFISILY GPLHLALGRP HTILLLLAPY FFFHFLSSNS GQYPSQRFAF
     PLLTKSMRNR SFQLVFLHSL LFQLFSLSVL GRPMLTRLSY IYIFRSNNKM LFVLSSFVGW
     LIGHILVLKW AGLVFVWLLK FIRSKTMKYI TCNVLIPATK YIIEKWRNSF VAGLIREILA
     MKQVESALVR IKNSRLLDDV RWWIRGSGLI SGLKINIRFY ARLILRGFEN VYVGAKFRQD
     MEHLFSIILF AFFLLYLDRT PLLYADPADK KLQLQRKLSN ETQAAREEKE LEERLTEKFE
     AQRRAQRAAQ RKALQEFKQG VVESYLANQA AKDENQIQAQ KDEKQIQAEK KATIRAEQVV
     QYTFGLIEAQ RREMEIEAAR AMQEAYKGML AAEEEDVEEG VQEKQEGFPE EPISPSEERE
     ENPKLLILKE KISILTEKIS ILTEKNDLFS FEIPIITSLF DPQKPLRPLR YIKTCAGVEK
     AVKNEMSQYF FYPCRSDGKQ RLCFTYPPSL ATFWEMIQRK MASRFPRIYT KAKWRALRGS
     APGSYRQWIS RNKKKKNSLS TEFQNRIQTL DQKKSLLNVL ARRKRSSLQN VLETRKRLCN
     YKTKKEYLPE IADPFLTGAL RGKSDPEVYD GVRKTSEIKV VFLKNNITMA TLGNKNDDDL
     REEKNAISLL SRMKNPVNKL HLLFVNERDY PFVKPLVNRI NGPAVPKKKK TISKSKQNEI
     KSKQKKVKSK QKKVKSKQKK VKSKQKKVKS KQNEIKSKQN EIKSKQKKVK RKQNEIKSKL
     NEVKRKQNEI YPKGVKFNAT PKTEINPHGI RFDAATIEKY SFATGYSYRP PSFHDIIFKA
     FVTEPQRNKK KVIELEEEIN KQVPRWSYQL IDELEQLEGA EGETQFSDHE IRILPFKRVS
     VFTEKEAKKK PLIDEEGNYV RHKKTYAVRF LGHMSDFRRG LIKGS