TI214_OENEH
ID TI214_OENEH Reviewed; 2463 AA.
AC Q9MTH5;
DT 10-OCT-2002, integrated into UniProtKB/Swiss-Prot.
DT 18-MAR-2008, sequence version 3.
DT 25-MAY-2022, entry version 64.
DE RecName: Full=Protein TIC 214 {ECO:0000250|UniProtKB:P56785};
DE AltName: Full=Translocon at the inner envelope membrane of chloroplasts 214 {ECO:0000250|UniProtKB:P56785};
DE Short=AtTIC214 {ECO:0000250|UniProtKB:P56785};
GN Name=TIC214 {ECO:0000250|UniProtKB:P56785}; Synonyms=ycf1;
OS Oenothera elata subsp. hookeri (Hooker's evening primrose) (Oenothera
OS hookeri).
OG Plastid; Chloroplast.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Myrtales; Onagraceae; Onagroideae; Onagreae; Oenothera.
OX NCBI_TaxID=85636;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Johansen;
RX PubMed=10852478; DOI=10.1007/pl00008686;
RA Hupfer H., Swiatek M., Hornung S., Herrmann R.G., Maier R.M., Chiu W.-L.,
RA Sears B.;
RT "Complete nucleotide sequence of the Oenothera elata plastid chromosome,
RT representing plastome I of the five distinguishable Euoenothera
RT plastomes.";
RL Mol. Gen. Genet. 263:581-585(2000).
RN [2]
RP SEQUENCE REVISION.
RX PubMed=18299283; DOI=10.1093/nar/gkn081;
RA Greiner S., Wang X., Rauwolf U., Silber M.V., Mayer K., Meurer J.,
RA Haberer G., Herrmann R.G.;
RT "The complete nucleotide sequences of the five genetically distinct plastid
RT genomes of Oenothera, subsection Oenothera: I. Sequence evaluation and
RT plastome evolution.";
RL Nucleic Acids Res. 36:2366-2378(2008).
CC -!- FUNCTION: Involved in protein precursor import into chloroplasts. May
CC be part of an intermediate translocation complex acting as a protein-
CC conducting channel at the inner envelope.
CC {ECO:0000250|UniProtKB:P56785}.
CC -!- SUBUNIT: Part of the Tic complex. {ECO:0000250|UniProtKB:P56785}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast inner membrane
CC {ECO:0000250|UniProtKB:P56785}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- SIMILARITY: Belongs to the TIC214 family. {ECO:0000305}.
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DR EMBL; AJ271079; CAB67227.3; -; Genomic_DNA.
DR RefSeq; NP_084759.2; NC_002693.2.
DR AlphaFoldDB; Q9MTH5; -.
DR PRIDE; Q9MTH5; -.
DR GeneID; 802789; -.
DR GO; GO:0009706; C:chloroplast inner membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR InterPro; IPR008896; TIC214.
DR PANTHER; PTHR33163; PTHR33163; 3.
DR Pfam; PF05758; Ycf1; 3.
PE 3: Inferred from homology;
KW Chloroplast; Coiled coil; Membrane; Plastid; Plastid inner membrane;
KW Protein transport; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..2463
FT /note="Protein TIC 214"
FT /id="PRO_0000217305"
FT TRANSMEM 18..38
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 60..80
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 86..106
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 127..147
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 170..190
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 297..317
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 792..841
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1230..1249
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1393..1417
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2116..2136
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2162..2187
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 326..441
FT /evidence="ECO:0000255"
FT COILED 2049..2192
FT /evidence="ECO:0000255"
FT COMPBIAS 792..827
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2162..2179
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 2463 AA; 291461 MW; 9964D1B084C3BBF8 CRC64;
MVNLVYVCMK INNSVVMVGL YYGFISAFSI GSSYLFLLRP RFLNDDPDAI EKKASETAGF
FTGQLLIFIS ILYGPLHLAL GRPHTILLLL APYFFFHYLF SNSGQWPSQR FAFPLLTKSM
RNRRFQLVFL NNLLFQLFSL SLLGRPMLTR LSYIYIFRCN NKMLFVLSSF VGWLIGHILV
LKWAGLVFVW LLQVIRSKTM KYITCNVLIP ATKYIIEKWR NSFVAGLIRE ILAMKQVESA
LVRIKNSKLL DDARWWIRGS SLISGLKINI RFYARLILRG FENVYVGAKF RQDMEHLFSI
ILFAIFLLYL DQTPLLYADP ADKKLQLQRK LSNETQAARA EKKLEERLTK KFEAQRRAQR
AAQRQALQEF KQGVVESYLA KQVAKDANQI QAQKDEKQIQ AEQKARRIRA EQVVQYTFWL
IEAQRREMEI EAARAMQEAY KGMLAAQEGY VEEGVQEKQE GFPEELISPS PIFHSEEREE
NPKLLILKEK ISILKKKISI LKKKISILKE KNDLFSFEIP IITSLFDPQK PLRPLRYIKT
CAGVEKAVKN EMSQYFFYAC RSDGKQRICF TYPPSLATFW EMIQRKMASR FPRIYAKAKW
RALRWSAPGS YRQWISRNKK KKNSLSTEFQ NRIKTLDKKK SLLNVLARRK RSSLLNVLAR
RKRSSLQNVL ETRKRLCNYK TNKTKKEYLP EKEYLPEIAD PFLTGALRGK SDPEVDDGGR
KTSDLIKVVF LKNNITMATL RNKNDDDLRE QKNAIALLSR MKNPVNKLHL LFVNERDYPF
VKTLVNRING PAVPKKKKKI SKSKQKNVKS KQKNVKSKQK NVKSKQKNVK SKQNEIKRKV
NEIKRKVNEI KRKQNESYPR GVKFGATPKT EINPHGIRFD AATIEKYSFA TGYSYSPPSF
DDILFHAFVT EPQRNKKAVI ELEEEINKKV PRWSYQLIDE LEQLEGAEGE TQFSDHEIRI
LPFKRVAVFT EKDSKKRKPL IDEQGNFVRH RKTYAIRFLG HMSDFRRGLI KGSARQDRRK
AYVCRTTQVN ARSPLFALGP RTFLDGLVNL AVQVKFFYET RIKGEKIVDD DDDNEKDEFK
VMIPDTKSIV AETREMLKQA GAEDGQSYED VEDDIRIENV TEMWENIDYG QVIRTFILLL
HIFLRKKVVF PAFIIGKNIA RMLLLQATEW KIDFARLKRE RYAICTYNGM KVSEKIAFDQ
FPPDWADDGI QILVTNPFYL KPWYRSKTRS IQKDPKKEKD PKKEKGPKKE PWYRFKTRFI
QKDPKKEKGP KKEKGPKKEP WYRRFFFQKD PKKEKGPKKG KAQFEGDRGV RFLTSFGILT
DRPFGDLITP DWGVFFNPIR NELKKKIRQF EKKHSIILSK RFRNVLKKTK KWFIKSFLFL
KRARLKRHPI ELSGGRETPE FTRSQKDIDN LKNEQDFRMS RNPRISESLL QGPVRALKDD
SLPEEKVADP EKEPSDLDNE LRAVWDEIDK VTKERKKIVF TPKPDSPDKL VQAKKNILKK
LERIKSRRHK FYFLRIRKSY YVLLFFIKRI SRNIKRIYLN PLERAISIRK IHPQRFFEFS
KKMIEKSIGI GKTETNKETV YKTKKKKKKK NPFISIFKES LYDKDIRISE NDIKLGDTWN
GYKYKRKKAT DTSDLASMSQ AYVFYKLYQT QQTQLIHLDK LRYVLQYDGT SRFLKKELKD
YFEAQELFHS KLKHKNSLNS GKNQWKNWLK AQHQYSVSPI IWNSLSPQKW RTKVNQERMD
ENTDLNKRYS NEKRKQFFEA NSLDDEENVV ETYLGQRAGD IKNSIKSYSY DLFSYQSINS
EDKYVCINNK QKNSYNYNRR KVNLVDSPEG IALSNQFLVQ NDLLDLYTFP DRKYVPWRLF
PGSLIGGNDK DKDRFVKMWT ATNSGNAVKY WTAANGNTSI KPGVFWTFQN SQRTKKQNPL
FDWRGMNTEL PNRCISDLKG WFFFSELLKL DLRYQVKPWI LSKNLLFENL IFENQEENPN
LIQNPIEDGR KNVIQNENEN DPIEDGRQNV IQNENENAIQ NLIDFFLEKK NSPKDTNQEL
HAQAKARIWD ALVASLKQKR EQKERKNKRI AQLIEKKKQK EIEKQKRKIE KQKRKKEKIE
NAKKKIENEK KKIETEEEKI EKEKRKKERK KEKLKKKVAK NIEKLKNKVA KNVAKNIEKL
KKQRAKNIAR MEEEDKKARK KRKRKVQVQE NKIPYTAFGS DKWQRPIAEY PKSGDIRNFQ
VILPEDDDED DEEDRLDELK LNAYELSRIQ KITDEKRMKR NLLSSIKRER LKMEFSTRNN
SLATIMLTHG IFSIEPLRIS RQNQDASFLI YQLIKISLVE QLDPYDHNDS FELTEKYRAR
RNFFMPKTNA ETMHKSDSDL FVPETILSTK RRRELRILIS FYSRRGKRKN RIYKNPVFWK
YVKNCGEVVD NSEKKKKKLI KSFLWPNYRL EDLACMNRYW FNAQNGSRFS MLRIRMYPRL
KIR
