ID   TI214_PINTH             Reviewed;        1756 AA.
AC   P41647;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   25-MAY-2022, entry version 66.
DE   RecName: Full=Protein TIC 214 {ECO:0000250|UniProtKB:P56785};
DE   AltName: Full=Translocon at the inner envelope membrane of chloroplasts 214 {ECO:0000250|UniProtKB:P56785};
DE            Short=AtTIC214 {ECO:0000250|UniProtKB:P56785};
GN   Name=TIC214 {ECO:0000250|UniProtKB:P56785}; Synonyms=ycf1;
OS   Pinus thunbergii (Japanese black pine) (Pinus thunbergiana).
OG   Plastid; Chloroplast.
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Pinopsida; Pinidae; Conifers I; Pinales; Pinaceae; Pinus;
OC   Pinus subgen. Pinus.
OX   NCBI_TaxID=3350;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=7937893; DOI=10.1073/pnas.91.21.9794;
RA   Wakasugi T., Tsudzuki J., Ito S., Nakashima K., Tsudzuki T., Sugiura M.;
RT   "Loss of all ndh genes as determined by sequencing the entire chloroplast
RT   genome of the black pine Pinus thunbergii.";
RL   Proc. Natl. Acad. Sci. U.S.A. 91:9794-9798(1994).
CC   -!- FUNCTION: Involved in protein precursor import into chloroplasts. May
CC       be part of an intermediate translocation complex acting as a protein-
CC       conducting channel at the inner envelope.
CC       {ECO:0000250|UniProtKB:P56785}.
CC   -!- SUBUNIT: Part of the Tic complex. {ECO:0000250|UniProtKB:P56785}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast inner membrane
CC       {ECO:0000250|UniProtKB:P56785}; Multi-pass membrane protein
CC       {ECO:0000255}.
CC   -!- SIMILARITY: Belongs to the TIC214 family. {ECO:0000305}.
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DR   EMBL; D17510; BAA04442.1; -; Genomic_DNA.
DR   PIR; T07566; T07566.
DR   RefSeq; NP_042487.1; NC_001631.1.
DR   AlphaFoldDB; P41647; -.
DR   PRIDE; P41647; -.
DR   GeneID; 1457612; -.
DR   GO; GO:0009706; C:chloroplast inner membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR   InterPro; IPR008896; TIC214.
DR   PANTHER; PTHR33163; PTHR33163; 3.
DR   Pfam; PF05758; Ycf1; 3.
PE   3: Inferred from homology;
KW   Chloroplast; Membrane; Plastid; Plastid inner membrane; Protein transport;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..1756
FT                   /note="Protein TIC 214"
FT                   /id="PRO_0000217303"
FT   TRANSMEM        18..38
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        54..74
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        79..99
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        128..148
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        163..183
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        210..230
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REGION          1469..1504
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1471..1486
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1756 AA;  205322 MW;  15C9946A8E8E7B56 CRC64;
     MIKVLGLLLG PLSSWVEVSG PIIIFGLYYG FLATLPFGPS KIYSTRSFLL GKPGYGIIAI
     SGSITGQLIG FLSMYYSPIY AALWKPYAIT LLVVPYMFFR FFQIMDKPSS SESPHLMNSI
     NNPKALSLFM DGLILQLLNP ILLANPVLTR LVNLFLFRYS PNISFMISGL CGWLGGHILL
     TIFIKWVSFR IDRNSLIDNT LLRRYINRTF SLLILCYCSF YLGRAPLPFL KGKNYEDNNG
     RSVTMARDDR SVAMARKNRS VAIDPQKLQE LIKEEKFFRQ QPWPIMCFDH NRVYQPIRYI
     GNSPFTRLGP VRTEVSQYFF GAYSSDGKKR ISFTFLPSVL VLGEKLVGGY RDFLYTSCSS
     EDPYYRWNHT VKRKRDSLGN EFSDRVKALS HGSPAENVIE RRVKFSNSNG DSFTEMYDPF
     LNGAFRGTIN QFESPQMLND SIISNLSDFI EIFMRDPKEG FPNDPLGYGY HISYLWQELE
     HESFPLPWEP LPTYTVRSLV PVTISSNPGT PQPKYALNRL KMKKISYLIQ TPEIQPPYWL
     WIAWRSSIIH VPVQVARCYG DIYTNFLVYV YGRFDRLIKP SASGIIRMDA ISCLFKKEYL
     FVYENLCFLF ECLAQYEWTI LLISRAGIPR DKRYSMEKKD FISLYREILL NEPLQIREIR
     KHVPRWSSGL MIGEYDDVDP VLLSSNRILS RKVKDTMTFD IGQRRIGVVQ NRYSAEADYR
     RNLIQGSIRA KRRKIMIWKR IQPNVHSLFF LKRKEIPAYP KDYYDTSDSG RVDKEQIGEE
     VREKIQRVED PLSQQTIAES LCLTWPELHY FRGLLLMAQS NIRKNIILPS LIIAKNMGRI
     LLFQVPEFYK DWEEMRREMH VICASDGTEL SETTFPDKWE TQGMQIKLLF PFRLKPWHRS
     KPQSAKRLRW SYLTTFGLET DIPFGDPTSN LGIFSKFLKP IFKKVKRRLM GSTGIRRVPR
     VGYIDKSELN DRIQNKLLSE TTPMGSANDS SEVNDQFGYE TQTINVKDQD DWTTTMKERI
     ESIAIINSSP ITGMSLVDSE IHTGSKGSFN ILGSTLKKRL VQIRRIPGLF RNKSVQLIRK
     RFYSIKFFIK RMDRRMDRDL LPSFINFIAS NIKFWIQSAW IRSTRNITTI YGRIFIINKD
     ISRINRGKIT NYYSINEKIQ DFEIRPDRNM FSMSQAYIFH KIWQIRALDI QRILDHEKPQ
     DLKENDWKQW LRCFDRYNLS PQIWSRINPN KCRNIVNKQC TCYEERFVPY EQQEDSIFAT
     VMEPFLGLLR KMNKRYRYDL LSYSYLNSTK DLDILNLTDI PGPPVQPAIP DERDITDREG
     ILKEKFINDI IEEDWKGTDF NIVNGSRSTV DIYDAIRSCD YDHTTSIDRQ KKKTDLITNQ
     QGIDETRREN VGIMDSPKIE KRISQLDLNF WLFPELSGIK NIYYETKSKF IPGNSLLREE
     RERKKIEEEE RKETTNVLEQ IIGIRSNVKN KQVEDGQDKN GQVEDQDGQD QDGQVEDQQT
     DGKKKTNKGL FQCKVVDVLG KKRFFYLANI CRVMSGMKDP GTYLRIQERN IDLSLMAFCI
     AIQKNSSANK ISKELALQRN VRGKVRNDNE IREDKKIMVF QPYRLSSIVD DQFLMYKIVS
     ISLKLKKRAG EWIDGDFYDG SVQRGKILGD EKNIFSSLNL EDILLPKRRR EFRILNRFDL
     ENDHVGFSNG KDIQNDEELM GRDQHLSVDT TQIIKRFLWP SYRLEDIICM NRYWFNTNDG
     SRSAMLRIRM YPLTVN