TI214_TOBAC
ID TI214_TOBAC Reviewed; 1901 AA.
AC P12222; P12220; P12224; Q32721; Q3HKA6;
DT 01-OCT-1989, integrated into UniProtKB/Swiss-Prot.
DT 01-APR-1993, sequence version 2.
DT 25-MAY-2022, entry version 84.
DE RecName: Full=Protein TIC 214 {ECO:0000250|UniProtKB:P56785};
DE AltName: Full=Translocon at the inner envelope membrane of chloroplasts 214 {ECO:0000250|UniProtKB:P56785};
DE Short=AtTIC214 {ECO:0000250|UniProtKB:P56785};
GN Name=TIC214 {ECO:0000250|UniProtKB:P56785}; Synonyms=ycf1;
OS Nicotiana tabacum (Common tobacco).
OG Plastid; Chloroplast.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC Nicotiana.
OX NCBI_TaxID=4097;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Bright Yellow 4;
RX PubMed=16453699; DOI=10.1002/j.1460-2075.1986.tb04464.x;
RA Shinozaki K., Ohme M., Tanaka M., Wakasugi T., Hayashida N.,
RA Matsubayashi T., Zaita N., Chunwongse J., Obokata J.,
RA Yamaguchi-Shinozaki K., Ohto C., Torazawa K., Meng B.-Y., Sugita M.,
RA Deno H., Kamogashira T., Yamada K., Kusuda J., Takaiwa F., Kato A.,
RA Tohdoh N., Shimada H., Sugiura M.;
RT "The complete nucleotide sequence of the tobacco chloroplast genome: its
RT gene organization and expression.";
RL EMBO J. 5:2043-2049(1986).
RN [2]
RP SEQUENCE REVISION.
RX PubMed=1731088; DOI=10.1016/0022-2836(92)90718-y;
RA Wolfe K.H., Morden C.W., Palmer J.D.;
RT "Small single-copy region of plastid DNA in the non-photosynthetic
RT angiosperm Epifagus virginiana contains only two genes. Differences among
RT dicots, monocots and bryophytes in gene organization at a non-bioenergetic
RT locus.";
RL J. Mol. Biol. 223:95-104(1992).
RN [3]
RP SEQUENCE REVISION.
RC STRAIN=cv. Bright Yellow 4;
RA Wakasugi T., Sugita M., Tsudzuki T., Sugiura M.;
RT "Updated gene map of tobacco chloroplast DNA.";
RL Plant Mol. Biol. Rep. 16:231-241(1998).
RN [4]
RP ESSENTIAL FOR GROWTH.
RC STRAIN=cv. Petit Havana;
RX PubMed=10792825; DOI=10.1046/j.1365-313x.2000.00722.x;
RA Drescher A., Ruf S., Calsa T. Jr., Carrer H., Bock R.;
RT "The two largest chloroplast genome-encoded open reading frames of higher
RT plants are essential genes.";
RL Plant J. 22:97-104(2000).
CC -!- FUNCTION: Involved in protein precursor import into chloroplasts. May
CC be part of an intermediate translocation complex acting as a protein-
CC conducting channel at the inner envelope.
CC {ECO:0000250|UniProtKB:P56785}.
CC -!- SUBUNIT: Part of the Tic complex. {ECO:0000250|UniProtKB:P56785}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast inner membrane
CC {ECO:0000250|UniProtKB:P56785}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- SIMILARITY: Belongs to the TIC214 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA77394.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; Z00044; CAA77394.1; ALT_SEQ; Genomic_DNA.
DR EMBL; Z00044; CAJ32485.1; -; Genomic_DNA.
DR PIR; A05036; A05036.
DR PIR; A05217; A05217.
DR PIR; A05218; A05218.
DR RefSeq; NP_054553.1; NC_001879.2.
DR AlphaFoldDB; P12222; -.
DR GeneID; 1466307; -.
DR KEGG; nta:1466307; -.
DR OMA; NEMSQYF; -.
DR OrthoDB; 53153at2759; -.
DR GO; GO:0009706; C:chloroplast inner membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR InterPro; IPR008896; TIC214.
DR PANTHER; PTHR33163; PTHR33163; 1.
DR Pfam; PF05758; Ycf1; 1.
PE 3: Inferred from homology;
KW Chloroplast; Membrane; Plastid; Plastid inner membrane; Protein transport;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..1901
FT /note="Protein TIC 214"
FT /id="PRO_0000217307"
FT TRANSMEM 18..38
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 64..84
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 87..107
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 124..144
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 172..192
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 221..241
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 248..299
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 797..817
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1591..1618
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 266..283
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1591..1610
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1901 AA; 226141 MW; F3E6727102B94B04 CRC64;
MIFQSFLLGN LVSLCMKIIN SVVVVGLYYG FLTTFSIGPS YLFLLRALVM EEGTEKKVSA
TTGFITGQLM MFISIYYAPL HLALGRPHTI TVLALPYLLF HFFWNNHKHF FDYGSTTRNS
MRNLSIQCVF LNNLIFQLFN HFILPSSMLA RLVNIYLFRC NSKILFVTSG FVGWLIGHIL
FMKWLGLVLV WIRQNHSIRS NKYIRSNKYL VLELRNSMAR IFSILLFITC VYYLGRIPSP
ILTKKLKEAS KTEERVESEE ERDVEIETAS EMKGTKQEQE GSTEEDPYPS PSLFSEERWD
PDKIDETEEI RVNGKDKIKD KFHSHLTETG YNNINTSNSP IYDYEDSYLN NNNTGNTEIF
KLQLLDKKNE NKDLFWFQQP LVSLLFDYNR WNRPFRYIKN NRFEQAIRTE MSQYFFNTCK
SDGKQRISFT YPPSLSTFWK MIKRRIPLLS LQKTLPNELD NQWISTNKEK SNNLNKEFLN
RLEVLDKESF SLDILETRTR LCNDDTKKEY VPKMYDPLLN GPYRGTIKKK FSPSIINNTS
LENLKERVRI NRIHTIFLPN TDYQELEQKV DTVAKKPLST EIDEFLTLIN EFGNEPKSSL
NLKDLSLFSD QEQGRVNSEK RTKFVKFVFN AIAPNGTTSE KKSIGIKEIS KKIPRWSHKL
ITELEQQSGD YQEGVPLDHQ IRSRKAKRVV IFTANNQNND PDTKDTDTAD QDQTKEVALI
RYSQQPDFRR GIIKGSMRAQ RRKTVIWKLF QANVHSPLFL DRITPPFLFS FDISGLIKPI
FRNWSGKEGE FKILESREEQ TKREEKKEKD KKGENKRKEK ARIEIAEAWD TIPFAQIIRG
YMLITQSILR KYIVLPSLII AKNLGRMLVL QLPEWSEDLQ EWNREMHIKC TYNGVQLSET
EFPKNWLKDG IQIKILFPFC LKPWHISKLY SSRGELMKKK KQKDDFCFLT VWGMEAELPF
GSPRKRPSFF EPIFKELEKK IGKFKKKYFI TLKVFKGKIK LFRRISKETK KWLIKSSLFI
KKMKKELSKV NPIVLFRLKE IDESNETKKE KDSLMSNQII NESFSQIESG NWPNSSLIES
KMKDLTDRTS TIKNQIERIT KEKKKVTPEI DISPNKTNNI KKFESPKKIF QILKRRNTRL
IWKFHYFLKL FIQRLYIDLF LSIINIPRIN TQLFLESTNK LIDKYISNNE INQEKINNQK
KIHFISTIKK SLYNISKKNS HIFFDLSYLS QAYVFYKLSQ PQVINLSKLR SVLQYNRTSF
FLKTKIKDYF RTLGIFHSEL KHKKLQSYRI NQWKNWLRRH YQYDLSQIRW SRLMPQKWRN
RVNQGCMAQN RNLNKWNSYE KDQLIHYKKE NDSELYSLAN QKDNFQKCYR YDLLAYKSIN
YEKKNDSFIS RLPFQVNKNL EISSNSNTSK HNLFDMLGNL HINNYLRKGN ILYIERNLDR
KYFDWKIIHF SLRQKEDIEA WVKIDTNSNP NTKIGINNYQ IIDKIDKKGF FYLTIHQNPE
NNQKNSKKAF FDWMGMNEKI LNRPILNLEF WFFPEFVPLY NVYKIKPWII PSKLLLLNLN
TNENVSQNKN INKNQKQNFF LRSNKKIKNR IQEAKEPASQ GEKERGSDIE NKGNLGPVLS
KHQNALKKDY AESDTKKGKK KKQYKSNTEA ELDLFLKRYL LFQLRWNDAL NQRMIENIKV
YCLLLRLINP SKIAISSIQR REMSLDIMLI QKNLTLTELM KKGILIIEPI RLSVKNNGQF
IMYQTIGISL VHKSKHQTNQ RYPEQRYVDK KNFDEFILQP QTQRINTDKN HFDLLVPENI
LWSRRRRELR IRSLFNSLNW NGIDRNSVFC NENNVKNWSQ FLDERKPLYK EKNELIKLKF
FLWPNYRLED LACMNRYWFD TNNGSRFSIL RIHMYPQLKI N
