TI221_ARATH
ID TI221_ARATH Reviewed; 173 AA.
AC A2RVP7; Q9SR75;
DT 06-FEB-2013, integrated into UniProtKB/Swiss-Prot.
DT 06-MAR-2007, sequence version 1.
DT 25-MAY-2022, entry version 86.
DE RecName: Full=Mitochondrial import inner membrane translocase subunit TIM22-1;
DE AltName: Full=Protein MATERNAL EFFECT EMBRYO ARREST 67;
DE Flags: Precursor;
GN Name=TIM22-1; Synonyms=MEE67; OrderedLocusNames=At3g10110;
GN ORFNames=T22K18.6;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=17098851; DOI=10.1104/pp.106.090688;
RA Murcha M.W., Elhafez D., Lister R., Tonti-Filippini J., Baumgartner M.,
RA Philippar K., Carrie C., Mokranjac D., Soll J., Whelan J.;
RT "Characterization of the preprotein and amino acid transporter gene family
RT in Arabidopsis.";
RL Plant Physiol. 143:199-212(2007).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Bautista V.R., Kim C.J., Chen H., Wu S.Y., De Los Reyes C., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (JAN-2007) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP TISSUE SPECIFICITY.
RX PubMed=12692332; DOI=10.1104/pp.102.016808;
RA Murcha M.W., Lister R., Ho A.Y., Whelan J.;
RT "Identification, expression, and import of components 17 and 23 of the
RT inner mitochondrial membrane translocase from Arabidopsis.";
RL Plant Physiol. 131:1737-1747(2003).
RN [6]
RP TISSUE SPECIFICITY, AND INDUCTION.
RX PubMed=14730085; DOI=10.1104/pp.103.033910;
RA Lister R., Chew O., Lee M.N., Heazlewood J.L., Clifton R., Parker K.L.,
RA Millar A.H., Whelan J.;
RT "A transcriptomic and proteomic characterization of the Arabidopsis
RT mitochondrial protein import apparatus and its response to mitochondrial
RT dysfunction.";
RL Plant Physiol. 134:777-789(2004).
RN [7]
RP IDENTIFICATION, AND DISRUPTION PHENOTYPE.
RX PubMed=15634699; DOI=10.1242/dev.01595;
RA Pagnussat G.C., Yu H.-J., Ngo Q.A., Rajani S., Mayalagu S., Johnson C.S.,
RA Capron A., Xie L.-F., Ye D., Sundaresan V.;
RT "Genetic and molecular identification of genes required for female
RT gametophyte development and function in Arabidopsis.";
RL Development 132:603-614(2005).
RN [8]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22223895; DOI=10.1074/mcp.m111.015131;
RA Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T.,
RA Giglione C.;
RT "Comparative large-scale characterisation of plant vs. mammal proteins
RT reveals similar and idiosyncratic N-alpha acetylation features.";
RL Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012).
CC -!- FUNCTION: Essential core component of the TIM22 complex, a complex that
CC mediates the import and insertion of multi-pass transmembrane proteins
CC into the mitochondrial inner membrane. {ECO:0000269|PubMed:17098851}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane {ECO:0000305};
CC Multi-pass membrane protein {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in young cotyledons, roots, flowers and
CC leaves. {ECO:0000269|PubMed:12692332, ECO:0000269|PubMed:14730085}.
CC -!- INDUCTION: Up-regulated after antimycin A or rotenone treatments.
CC {ECO:0000269|PubMed:14730085}.
CC -!- DISRUPTION PHENOTYPE: Embryo lethality. {ECO:0000269|PubMed:15634699}.
CC -!- SIMILARITY: Belongs to the Tim17/Tim22/Tim23 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF04413.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; DQ405268; ABD64057.1; -; mRNA.
DR EMBL; DQ405269; ABD64058.1; -; mRNA.
DR EMBL; AC010927; AAF04413.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002686; AEE74859.1; -; Genomic_DNA.
DR EMBL; BT030038; ABN04776.1; -; mRNA.
DR RefSeq; NP_566368.1; NM_111846.3.
DR AlphaFoldDB; A2RVP7; -.
DR SMR; A2RVP7; -.
DR STRING; 3702.AT3G10110.1; -.
DR TCDB; 3.A.8.1.3; the mitochondrial protein translocase (mpt) family.
DR PaxDb; A2RVP7; -.
DR EnsemblPlants; AT3G10110.1; AT3G10110.1; AT3G10110.
DR GeneID; 820172; -.
DR Gramene; AT3G10110.1; AT3G10110.1; AT3G10110.
DR KEGG; ath:AT3G10110; -.
DR Araport; AT3G10110; -.
DR TAIR; locus:2100088; AT3G10110.
DR eggNOG; KOG3225; Eukaryota.
DR HOGENOM; CLU_091077_2_0_1; -.
DR InParanoid; A2RVP7; -.
DR OMA; QGMESCA; -.
DR OrthoDB; 1544601at2759; -.
DR PhylomeDB; A2RVP7; -.
DR PRO; PR:A2RVP7; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; A2RVP7; baseline and differential.
DR Genevisible; A2RVP7; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0042721; C:TIM22 mitochondrial import inner membrane insertion complex; IBA:GO_Central.
DR GO; GO:0030943; F:mitochondrion targeting sequence binding; IBA:GO_Central.
DR GO; GO:0008320; F:protein transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0009793; P:embryo development ending in seed dormancy; IMP:TAIR.
DR GO; GO:0045039; P:protein insertion into mitochondrial inner membrane; IBA:GO_Central.
DR InterPro; IPR039175; TIM22.
DR PANTHER; PTHR14110; PTHR14110; 1.
PE 1: Evidence at protein level;
KW Membrane; Mitochondrion; Mitochondrion inner membrane; Reference proteome;
KW Transit peptide; Transmembrane; Transmembrane helix.
FT TRANSIT 1..18
FT /note="Mitochondrion"
FT /evidence="ECO:0000255"
FT CHAIN 19..173
FT /note="Mitochondrial import inner membrane translocase
FT subunit TIM22-1"
FT /id="PRO_0000420934"
FT TRANSMEM 52..72
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 101..119
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 128..144
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 151..168
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..26
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 9
FT /note="P -> T (in Ref. 1; ABD64057/ABD64058)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 173 AA; 18183 MW; CFC24979387481AB CRC64;
MADSSAAEPT TGASSPPVAS DENSTQIQPI RMPTIEEIRA QEVWNNCAVR AVTSGVMGGG
LGLMMGLFLG ALDNPITHDT MTARQQFVFT AKQMGQRSWN SCKTFAVMGL VFSAAECIVE
KARAKHDTVN TAIAGCVTGG SMSARGGPKA ACIGCAGFAT FSVLIEKFFD RHT
