TI223_ARATH
ID TI223_ARATH Reviewed; 214 AA.
AC Q6NKU9; Q9FJ60;
DT 06-FEB-2013, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 107.
DE RecName: Full=Mitochondrial import inner membrane translocase subunit TIM22-3;
GN Name=TIM22-3; OrderedLocusNames=At5g55510; ORFNames=MTE17.23;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=17098851; DOI=10.1104/pp.106.090688;
RA Murcha M.W., Elhafez D., Lister R., Tonti-Filippini J., Baumgartner M.,
RA Philippar K., Carrie C., Mokranjac D., Soll J., Whelan J.;
RT "Characterization of the preprotein and amino acid transporter gene family
RT in Arabidopsis.";
RL Plant Physiol. 143:199-212(2007).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9872454; DOI=10.1093/dnares/5.5.297;
RA Nakamura Y., Sato S., Asamizu E., Kaneko T., Kotani H., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. VII. Sequence
RT features of the regions of 1,013,767 bp covered by sixteen physically
RT assigned P1 and TAC clones.";
RL DNA Res. 5:297-308(1998).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Cheuk R., Chen H., Kim C.J., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP TISSUE SPECIFICITY.
RX PubMed=12692332; DOI=10.1104/pp.102.016808;
RA Murcha M.W., Lister R., Ho A.Y., Whelan J.;
RT "Identification, expression, and import of components 17 and 23 of the
RT inner mitochondrial membrane translocase from Arabidopsis.";
RL Plant Physiol. 131:1737-1747(2003).
RN [7]
RP TISSUE SPECIFICITY, AND INDUCTION.
RX PubMed=14730085; DOI=10.1104/pp.103.033910;
RA Lister R., Chew O., Lee M.N., Heazlewood J.L., Clifton R., Parker K.L.,
RA Millar A.H., Whelan J.;
RT "A transcriptomic and proteomic characterization of the Arabidopsis
RT mitochondrial protein import apparatus and its response to mitochondrial
RT dysfunction.";
RL Plant Physiol. 134:777-789(2004).
CC -!- FUNCTION: Essential core component of the TIM22 complex, a complex that
CC mediates the import and insertion of multi-pass transmembrane proteins
CC into the mitochondrial inner membrane. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane {ECO:0000305};
CC Multi-pass membrane protein {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in young cotyledons, roots, flowers and
CC leaves. {ECO:0000269|PubMed:12692332, ECO:0000269|PubMed:14730085}.
CC -!- INDUCTION: Up-regulated after antimycin A or rotenone treatments.
CC {ECO:0000269|PubMed:14730085}.
CC -!- SIMILARITY: Belongs to the Tim17/Tim22/Tim23 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB08568.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; DQ405271; ABD64060.1; -; mRNA.
DR EMBL; AB015479; BAB08568.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002688; AED96638.1; -; Genomic_DNA.
DR EMBL; BT012594; AAT06413.1; -; mRNA.
DR EMBL; BT015156; AAT85752.1; -; mRNA.
DR EMBL; AK227469; BAE99471.1; -; mRNA.
DR RefSeq; NP_200362.3; NM_124933.5.
DR AlphaFoldDB; Q6NKU9; -.
DR SMR; Q6NKU9; -.
DR STRING; 3702.AT5G55510.1; -.
DR PaxDb; Q6NKU9; -.
DR PRIDE; Q6NKU9; -.
DR ProteomicsDB; 234399; -.
DR DNASU; 835645; -.
DR EnsemblPlants; AT5G55510.1; AT5G55510.1; AT5G55510.
DR GeneID; 835645; -.
DR Gramene; AT5G55510.1; AT5G55510.1; AT5G55510.
DR KEGG; ath:AT5G55510; -.
DR Araport; AT5G55510; -.
DR TAIR; locus:2173907; AT5G55510.
DR eggNOG; KOG1652; Eukaryota.
DR HOGENOM; CLU_083527_0_0_1; -.
DR InParanoid; Q6NKU9; -.
DR OMA; NRDANDM; -.
DR OrthoDB; 1456214at2759; -.
DR PhylomeDB; Q6NKU9; -.
DR PRO; PR:Q6NKU9; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q6NKU9; baseline and differential.
DR GO; GO:0009507; C:chloroplast; IDA:TAIR.
DR GO; GO:0009941; C:chloroplast envelope; HDA:TAIR.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0042721; C:TIM22 mitochondrial import inner membrane insertion complex; IEA:InterPro.
DR GO; GO:0008320; F:protein transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0045039; P:protein insertion into mitochondrial inner membrane; IEA:InterPro.
DR GO; GO:0045036; P:protein targeting to chloroplast; IBA:GO_Central.
DR GO; GO:0015031; P:protein transport; IDA:TAIR.
DR InterPro; IPR039175; TIM22.
DR PANTHER; PTHR14110; PTHR14110; 1.
PE 2: Evidence at transcript level;
KW Membrane; Mitochondrion; Mitochondrion inner membrane; Reference proteome;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..214
FT /note="Mitochondrial import inner membrane translocase
FT subunit TIM22-3"
FT /id="PRO_0000420936"
FT TRANSMEM 38..61
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 88..105
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 114..130
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 137..154
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..35
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..17
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 18..32
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 214 AA; 22524 MW; 704656E8EB164277 CRC64;
MAAENSSNAI NVDTSLDSDS KPNRDANDMT DHDSSSKALV IPAPAVCLVR FAGDAASGAF
MGSVFGYGSG LFKKKGFKGS FVDAGQSAKT FAVLSGVHSL VVCLLKQIRG KDDAINVGVA
GCCTGLALSF PGAPQAMLQS CLTFGAFSFI LEGLNKRQTA LAHSVSFRQQ TRSPQHDLPL
LSLAIPIHDE IKGAFSSFCN SLTKPKKLKF PHAR
