ID   TI224_ARATH             Reviewed;         142 AA.
AC   A1XJK0; F4IAQ1;
DT   06-FEB-2013, integrated into UniProtKB/Swiss-Prot.
DT   06-MAR-2013, sequence version 3.
DT   03-AUG-2022, entry version 83.
DE   RecName: Full=Mitochondrial import inner membrane translocase subunit TIM22-4;
GN   Name=TIM22-4; OrderedLocusNames=At1g18320; ORFNames=F15H18.28;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11130712; DOI=10.1038/35048500;
RA   Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA   Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA   Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA   Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA   Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA   Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA   Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA   Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA   Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA   Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA   Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA   Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA   Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA   Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA   Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT   "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL   Nature 408:816-820(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA   Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA   Feldmann K.A.;
RT   "Full-length cDNA from Arabidopsis thaliana.";
RL   Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Essential core component of the TIM22 complex, a complex that
CC       mediates the import and insertion of multi-pass transmembrane proteins
CC       into the mitochondrial inner membrane. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane {ECO:0000305};
CC       Multi-pass membrane protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the Tim17/Tim22/Tim23 family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AY089200; Type=Frameshift; Evidence={ECO:0000305};
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DR   EMBL; AC013354; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; CP002684; AEE29701.1; -; Genomic_DNA.
DR   EMBL; AY089200; -; NOT_ANNOTATED_CDS; mRNA.
DR   RefSeq; NP_173268.3; NM_101690.3.
DR   AlphaFoldDB; A1XJK0; -.
DR   SMR; A1XJK0; -.
DR   STRING; 3702.AT1G18320.1; -.
DR   PaxDb; A1XJK0; -.
DR   PRIDE; A1XJK0; -.
DR   GeneID; 838412; -.
DR   KEGG; ath:AT1G18320; -.
DR   Araport; AT1G18320; -.
DR   TAIR; locus:2014139; AT1G18320.
DR   eggNOG; KOG3225; Eukaryota.
DR   HOGENOM; CLU_091077_1_0_1; -.
DR   InParanoid; A1XJK0; -.
DR   OrthoDB; 1544601at2759; -.
DR   PRO; PR:A1XJK0; -.
DR   Proteomes; UP000006548; Chromosome 1.
DR   ExpressionAtlas; A1XJK0; baseline and differential.
DR   Genevisible; A1XJK0; AT.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0042721; C:TIM22 mitochondrial import inner membrane insertion complex; IBA:GO_Central.
DR   GO; GO:0030943; F:mitochondrion targeting sequence binding; IBA:GO_Central.
DR   GO; GO:0008320; F:protein transmembrane transporter activity; IBA:GO_Central.
DR   GO; GO:0045039; P:protein insertion into mitochondrial inner membrane; IBA:GO_Central.
DR   InterPro; IPR039175; TIM22.
DR   PANTHER; PTHR14110; PTHR14110; 1.
PE   2: Evidence at transcript level;
KW   Membrane; Mitochondrion; Mitochondrion inner membrane; Reference proteome;
KW   Transmembrane; Transmembrane helix.
FT   CHAIN           1..142
FT                   /note="Mitochondrial import inner membrane translocase
FT                   subunit TIM22-4"
FT                   /id="PRO_0000420937"
FT   TRANSMEM        21..41
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        70..88
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        97..113
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        120..137
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   142 AA;  15097 MW;  49B954C61F4A5DB0 CRC64;
     MPTIEEIRAQ EVWNNCAVRA VTSGVMGGGL GLMMGLFLGA LDNPITHDTM TARQQFVFTA
     KQMGQRSWNS CKTFAVMGLV FSAAECIVEK ARAKHDTVNT AIAGCVTGGS MSARGGPKAA
     CIGCAGFAIF SVLIEKFFDR HT