TI232_ARATH
ID TI232_ARATH Reviewed; 188 AA.
AC Q38820;
DT 06-FEB-2013, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 124.
DE RecName: Full=Mitochondrial import inner membrane translocase subunit TIM23-2;
GN Name=TIM23-2; OrderedLocusNames=At1g72750; ORFNames=F28P22.6;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Columbia;
RA Almira E.C., Lu G., Ferl R.J.;
RT "A gene encoding an inner mitochondrial membrane protein in Arabidopsis
RT thaliana.";
RL Submitted (DEC-1994) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, AND
RP SUBCELLULAR LOCATION.
RX PubMed=12692332; DOI=10.1104/pp.102.016808;
RA Murcha M.W., Lister R., Ho A.Y., Whelan J.;
RT "Identification, expression, and import of components 17 and 23 of the
RT inner mitochondrial membrane translocase from Arabidopsis.";
RL Plant Physiol. 131:1737-1747(2003).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [7]
RP TISSUE SPECIFICITY, SUBCELLULAR LOCATION, AND IDENTIFICATION BY MASS
RP SPECTROMETRY.
RX PubMed=14730085; DOI=10.1104/pp.103.033910;
RA Lister R., Chew O., Lee M.N., Heazlewood J.L., Clifton R., Parker K.L.,
RA Millar A.H., Whelan J.;
RT "A transcriptomic and proteomic characterization of the Arabidopsis
RT mitochondrial protein import apparatus and its response to mitochondrial
RT dysfunction.";
RL Plant Physiol. 134:777-789(2004).
RN [8]
RP SUBCELLULAR LOCATION.
RX PubMed=17098851; DOI=10.1104/pp.106.090688;
RA Murcha M.W., Elhafez D., Lister R., Tonti-Filippini J., Baumgartner M.,
RA Philippar K., Carrie C., Mokranjac D., Soll J., Whelan J.;
RT "Characterization of the preprotein and amino acid transporter gene family
RT in Arabidopsis.";
RL Plant Physiol. 143:199-212(2007).
RN [9]
RP INTERACTION WITH OEP163; TIM17-2; TIM21; TIM50 AND MPPA2, SUBUNIT, AND
RP SUBCELLULAR LOCATION.
RX PubMed=22730406; DOI=10.1105/tpc.112.098731;
RA Wang Y., Carrie C., Giraud E., Elhafez D., Narsai R., Duncan O., Whelan J.,
RA Murcha M.W.;
RT "Dual location of the mitochondrial preprotein transporters B14.7 and
RT Tim23-2 in complex I and the TIM17:23 complex in Arabidopsis links
RT mitochondrial activity and biogenesis.";
RL Plant Cell 24:2675-2695(2012).
CC -!- FUNCTION: Essential component of the TIM17:23 complex, a complex that
CC mediates the translocation of transit peptide-containing proteins
CC across the mitochondrial inner membrane. Links the inner and outer
CC membranes (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Homomultimer. Component of the TIM17:23 complex at least
CC composed of TIM23, TIM17 and TIM50. The complex interacts with the
CC TIM44 component of the PAM complex. Also part of the NADH-ubiquinone
CC oxidoreductase complex I. Interacts with OEP163, TIM17-2, TIM21, TIM50
CC and MPPA2. {ECO:0000269|PubMed:22730406}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane {ECO:0000305};
CC Multi-pass membrane protein {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in roots and young cotyledons. Detected
CC in leaves and flowers. {ECO:0000269|PubMed:12692332,
CC ECO:0000269|PubMed:14730085}.
CC -!- DEVELOPMENTAL STAGE: Peak of expression during cotyledon development.
CC {ECO:0000269|PubMed:12692332}.
CC -!- DOMAIN: The C-terminal part (143-188) is required for insertion in the
CC mitochondrial inner membrane.
CC -!- SIMILARITY: Belongs to the Tim17/Tim22/Tim23 family. {ECO:0000305}.
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DR EMBL; U18126; AAA57314.1; -; mRNA.
DR EMBL; AY463973; AAR26374.1; -; mRNA.
DR EMBL; AC010926; AAG51855.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE35368.1; -; Genomic_DNA.
DR EMBL; AY072498; AAL66913.1; -; mRNA.
DR EMBL; AY042892; AAK68832.1; -; mRNA.
DR EMBL; AY088456; AAM65992.1; -; mRNA.
DR PIR; S71194; S71194.
DR RefSeq; NP_177419.1; NM_105934.2.
DR AlphaFoldDB; Q38820; -.
DR SMR; Q38820; -.
DR BioGRID; 28826; 7.
DR STRING; 3702.AT1G72750.1; -.
DR TCDB; 3.A.8.1.4; the mitochondrial protein translocase (mpt) family.
DR SwissPalm; Q38820; -.
DR PaxDb; Q38820; -.
DR PRIDE; Q38820; -.
DR ProteomicsDB; 246485; -.
DR EnsemblPlants; AT1G72750.1; AT1G72750.1; AT1G72750.
DR GeneID; 843607; -.
DR Gramene; AT1G72750.1; AT1G72750.1; AT1G72750.
DR KEGG; ath:AT1G72750; -.
DR Araport; AT1G72750; -.
DR TAIR; locus:2030260; AT1G72750.
DR eggNOG; KOG3324; Eukaryota.
DR HOGENOM; CLU_118311_0_0_1; -.
DR InParanoid; Q38820; -.
DR OMA; AVMYSGF; -.
DR OrthoDB; 1418527at2759; -.
DR PhylomeDB; Q38820; -.
DR PRO; PR:Q38820; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q38820; baseline and differential.
DR Genevisible; Q38820; AT.
DR GO; GO:0031305; C:integral component of mitochondrial inner membrane; IBA:GO_Central.
DR GO; GO:0005743; C:mitochondrial inner membrane; HDA:TAIR.
DR GO; GO:0005739; C:mitochondrion; HDA:TAIR.
DR GO; GO:0005744; C:TIM23 mitochondrial import inner membrane translocase complex; IBA:GO_Central.
DR GO; GO:0008320; F:protein transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0030150; P:protein import into mitochondrial matrix; IBA:GO_Central.
DR InterPro; IPR005681; Tim23.
DR InterPro; IPR045238; Tim23-like.
DR PANTHER; PTHR15371; PTHR15371; 1.
DR TIGRFAMs; TIGR00983; 3a0801s02tim23; 1.
PE 1: Evidence at protein level;
KW Membrane; Mitochondrion; Mitochondrion inner membrane; Reference proteome;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..188
FT /note="Mitochondrial import inner membrane translocase
FT subunit TIM23-2"
FT /id="PRO_0000420939"
FT TRANSMEM 64..84
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 112..131
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 138..154
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 161..178
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 188 AA; 19878 MW; E7E0634175E431BE CRC64;
MAANNRSDHG SDENTRLYNP YQNYEVPINK SQYLYKLPTS PEFLFTEEAL RQRRSWGENL
TFYTGTAYLG GSVAGASVGV ITGVKSFESG DTTKLKINRI LNSSGQTGRT WGNRIGIIGL
VYAGIESGIV AATDRDDVWT SVVAGLGTGA VCRAARGVRS AAVAGALGGL AAGAVVAGKQ
IVKRYVPI