ID   TI233_ARATH             Reviewed;         188 AA.
AC   Q9S837;
DT   06-FEB-2013, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   03-AUG-2022, entry version 118.
DE   RecName: Full=Mitochondrial import inner membrane translocase subunit TIM23-3;
GN   Name=TIM23-3; OrderedLocusNames=At3g04800; ORFNames=F7O18.29, T9J14.25;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, AND SUBCELLULAR LOCATION.
RX   PubMed=12692332; DOI=10.1104/pp.102.016808;
RA   Murcha M.W., Lister R., Ho A.Y., Whelan J.;
RT   "Identification, expression, and import of components 17 and 23 of the
RT   inner mitochondrial membrane translocase from Arabidopsis.";
RL   Plant Physiol. 131:1737-1747(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11130713; DOI=10.1038/35048706;
RA   Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA   Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA   Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA   Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA   Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA   Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA   Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA   Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA   Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA   Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA   Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA   Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA   de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA   Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA   Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA   Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA   Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA   Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA   Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA   Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA   Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA   Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA   Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA   Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT   "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL   Nature 408:820-822(2000).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA   Kim C.J., Chen H., Quinitio C., Shinn P., Ecker J.R.;
RT   "Arabidopsis ORF clones.";
RL   Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   TISSUE SPECIFICITY.
RX   PubMed=14730085; DOI=10.1104/pp.103.033910;
RA   Lister R., Chew O., Lee M.N., Heazlewood J.L., Clifton R., Parker K.L.,
RA   Millar A.H., Whelan J.;
RT   "A transcriptomic and proteomic characterization of the Arabidopsis
RT   mitochondrial protein import apparatus and its response to mitochondrial
RT   dysfunction.";
RL   Plant Physiol. 134:777-789(2004).
RN   [6]
RP   SUBCELLULAR LOCATION.
RX   PubMed=17098851; DOI=10.1104/pp.106.090688;
RA   Murcha M.W., Elhafez D., Lister R., Tonti-Filippini J., Baumgartner M.,
RA   Philippar K., Carrie C., Mokranjac D., Soll J., Whelan J.;
RT   "Characterization of the preprotein and amino acid transporter gene family
RT   in Arabidopsis.";
RL   Plant Physiol. 143:199-212(2007).
CC   -!- FUNCTION: Essential component of the TIM17:23 complex, a complex that
CC       mediates the translocation of transit peptide-containing proteins
CC       across the mitochondrial inner membrane. Links the inner and outer
CC       membranes (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the TIM17:23 complex at least composed of TIM23,
CC       TIM17 and TIM50. The complex interacts with the TIM44 component of the
CC       PAM complex (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane {ECO:0000305};
CC       Multi-pass membrane protein {ECO:0000305}.
CC   -!- TISSUE SPECIFICITY: Expressed in roots. Detected in cotyledons, leaves
CC       and flowers. {ECO:0000269|PubMed:12692332,
CC       ECO:0000269|PubMed:14730085}.
CC   -!- SIMILARITY: Belongs to the Tim17/Tim22/Tim23 family. {ECO:0000305}.
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DR   EMBL; AY463974; AAR26375.1; -; mRNA.
DR   EMBL; AC009465; AAG51426.1; -; Genomic_DNA.
DR   EMBL; AC011437; AAF04906.1; -; Genomic_DNA.
DR   EMBL; CP002686; AEE74137.1; -; Genomic_DNA.
DR   EMBL; BT025747; ABF83637.1; -; mRNA.
DR   RefSeq; NP_187131.1; NM_111352.1.
DR   AlphaFoldDB; Q9S837; -.
DR   SMR; Q9S837; -.
DR   STRING; 3702.AT3G04800.1; -.
DR   iPTMnet; Q9S837; -.
DR   PaxDb; Q9S837; -.
DR   PRIDE; Q9S837; -.
DR   ProteomicsDB; 234425; -.
DR   EnsemblPlants; AT3G04800.1; AT3G04800.1; AT3G04800.
DR   GeneID; 819640; -.
DR   Gramene; AT3G04800.1; AT3G04800.1; AT3G04800.
DR   KEGG; ath:AT3G04800; -.
DR   Araport; AT3G04800; -.
DR   TAIR; locus:2084913; AT3G04800.
DR   eggNOG; KOG3324; Eukaryota.
DR   HOGENOM; CLU_118311_0_0_1; -.
DR   InParanoid; Q9S837; -.
DR   OMA; KYRQYNP; -.
DR   OrthoDB; 1418527at2759; -.
DR   PhylomeDB; Q9S837; -.
DR   PRO; PR:Q9S837; -.
DR   Proteomes; UP000006548; Chromosome 3.
DR   ExpressionAtlas; Q9S837; baseline and differential.
DR   Genevisible; Q9S837; AT.
DR   GO; GO:0031305; C:integral component of mitochondrial inner membrane; IBA:GO_Central.
DR   GO; GO:0005744; C:TIM23 mitochondrial import inner membrane translocase complex; IBA:GO_Central.
DR   GO; GO:0008320; F:protein transmembrane transporter activity; IBA:GO_Central.
DR   GO; GO:0030150; P:protein import into mitochondrial matrix; IBA:GO_Central.
DR   InterPro; IPR045238; Tim23-like.
DR   PANTHER; PTHR15371; PTHR15371; 1.
PE   2: Evidence at transcript level;
KW   Membrane; Mitochondrion; Mitochondrion inner membrane; Reference proteome;
KW   Transmembrane; Transmembrane helix.
FT   CHAIN           1..188
FT                   /note="Mitochondrial import inner membrane translocase
FT                   subunit TIM23-3"
FT                   /id="PRO_0000420940"
FT   TRANSMEM        63..83
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        111..130
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        138..154
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        161..178
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   188 AA;  20213 MW;  09BD1C88A53324C8 CRC64;
     MADPMNHSTG HQQQQKYRQY NPYQQVNLPY RKLYELPTSP EFLFEEEATK KRLTWGENLT
     FFTGWGYCTG SVLGAFKGTI AGMRAAERGE SLKIRTNRIL NSGGLVARRG GNCLGSVGLM
     FAAMESGVTY MRDGDDGSLT TVIAGLATGV LYRAASGPRS AVVAGAVGGV AALAAVAGRR
     IVKRFVPI