TI236_ARATH
ID TI236_ARATH Reviewed; 2166 AA.
AC F4ISL7; Q9SL97;
DT 05-JUN-2019, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 03-AUG-2022, entry version 77.
DE RecName: Full=Protein TIC236, chloroplastic {ECO:0000305};
DE AltName: Full=236 kDa translocon at the inner-envelope-membrane of chloroplasts {ECO:0000305};
DE AltName: Full=Protein EMBRYO DEFECTIVE 2410 {ECO:0000303|PubMed:15266054};
DE Flags: Precursor;
GN Name=TIC236 {ECO:0000303|PubMed:30464337};
GN Synonyms=EMB2410 {ECO:0000303|PubMed:15266054};
GN OrderedLocusNames=At2g25660 {ECO:0000312|Araport:AT2G25660};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP DISRUPTION PHENOTYPE.
RX PubMed=15266054; DOI=10.1104/pp.104.045179;
RA Tzafrir I., Pena-Muralla R., Dickerman A., Berg M., Rogers R., Hutchens S.,
RA Sweeney T.C., McElver J., Aux G., Patton D., Meinke D.;
RT "Identification of genes required for embryo development in Arabidopsis.";
RL Plant Physiol. 135:1206-1220(2004).
RN [4]
RP FUNCTION, INTERACTION WITH TOC75-3, SUBCELLULAR LOCATION, AND TOPOLOGY.
RX PubMed=30464337; DOI=10.1038/s41586-018-0713-y;
RA Chen Y.L., Chen L.J., Chu C.C., Huang P.K., Wen J.R., Li H.M.;
RT "TIC236 links the outer and inner membrane translocons of the
RT chloroplast.";
RL Nature 564:125-129(2018).
CC -!- FUNCTION: Part of the inner chloroplast membrane translocon complex
CC (TIC) which associates with the outer chloroplast membrane translocon
CC complex (TOC) and forms a supercomplex involved in protein precursor
CC import into the chloroplast stroma (PubMed:30464337). Required for the
CC import of HSP93, TIC40 and RBCS protein precursors in the chloroplast
CC stroma (PubMed:30464337). Links the outer and inner membrane
CC translocons of the chloroplast envelope (PubMed:30464337).
CC {ECO:0000269|PubMed:30464337}.
CC -!- SUBUNIT: Part of the TIC complex, which can interact with components of
CC the TOC complex to form a larger import complex (PubMed:30464337).
CC Interacts with the TOC complex component TOC75-3 (PubMed:30464337).
CC {ECO:0000269|PubMed:30464337}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast inner membrane
CC {ECO:0000269|PubMed:30464337}; Single-pass membrane protein
CC {ECO:0000255}. Plastid, chloroplast intermembrane space
CC {ECO:0000269|PubMed:30464337}.
CC -!- DISRUPTION PHENOTYPE: Embryonic lethality when homozygous
CC (PubMed:15266054). Embryo development arrested at globular stage
CC (PubMed:15266054). {ECO:0000269|PubMed:15266054}.
CC -!- SIMILARITY: Belongs to the TamB family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAD31376.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC006053; AAD31376.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002685; AEC07731.1; -; Genomic_DNA.
DR EMBL; CP002685; ANM61810.1; -; Genomic_DNA.
DR PIR; B84651; B84651.
DR RefSeq; NP_001324007.1; NM_001336009.1.
DR RefSeq; NP_180137.3; NM_128125.3.
DR AlphaFoldDB; F4ISL7; -.
DR STRING; 3702.AT2G25660.1; -.
DR iPTMnet; F4ISL7; -.
DR PaxDb; F4ISL7; -.
DR PRIDE; F4ISL7; -.
DR ProteomicsDB; 189734; -.
DR EnsemblPlants; AT2G25660.1; AT2G25660.1; AT2G25660.
DR EnsemblPlants; AT2G25660.4; AT2G25660.4; AT2G25660.
DR GeneID; 817107; -.
DR Gramene; AT2G25660.1; AT2G25660.1; AT2G25660.
DR Gramene; AT2G25660.4; AT2G25660.4; AT2G25660.
DR KEGG; ath:AT2G25660; -.
DR Araport; AT2G25660; -.
DR TAIR; locus:2050327; AT2G25660.
DR eggNOG; ENOG502QTMX; Eukaryota.
DR HOGENOM; CLU_000678_0_0_1; -.
DR InParanoid; F4ISL7; -.
DR OMA; ANGHIKF; -.
DR OrthoDB; 35018at2759; -.
DR PRO; PR:F4ISL7; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; F4ISL7; baseline and differential.
DR GO; GO:0009501; C:amyloplast; IEA:EnsemblPlants.
DR GO; GO:0009706; C:chloroplast inner membrane; IDA:TAIR.
DR GO; GO:0031972; C:chloroplast intermembrane space; IEA:UniProtKB-SubCell.
DR GO; GO:0005887; C:integral component of plasma membrane; IEA:InterPro.
DR GO; GO:0009660; P:amyloplast organization; IEA:EnsemblPlants.
DR GO; GO:0009306; P:protein secretion; IEA:InterPro.
DR InterPro; IPR007452; TamB.
DR Pfam; PF04357; TamB; 1.
PE 1: Evidence at protein level;
KW Chloroplast; Membrane; Plastid; Plastid inner membrane; Protein transport;
KW Reference proteome; Transit peptide; Transmembrane; Transmembrane helix;
KW Transport.
FT TRANSIT 1..37
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 38..2166
FT /note="Protein TIC236, chloroplastic"
FT /id="PRO_0000447404"
FT TOPO_DOM 38..101
FT /note="Stromal"
FT /evidence="ECO:0000305|PubMed:30464337"
FT TRANSMEM 102..122
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 123..2166
FT /note="Chloroplast intermembrane"
FT /evidence="ECO:0000305|PubMed:30464337"
FT REGION 1611..1649
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1627..1649
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 2166 AA; 240344 MW; 9FBCB0DB12226B86 CRC64;
MSLRLQNPFL STPLLHGSFN RREKRINVAR RAFRSKRIYS EKKQNDWLAK VAKFSQFCGK
NVQLLRKSLD SRSRMEVKCL KEPFVRSKDL VRSLAPVWEE GLFFLRCSVF FAVISGVCLL
VWYGQNKARV FVETKLLPSV CSVLSETIQR EVDFGKVRRV SPLCITLEAS SIGPHGEEFS
CGEVPTMKVC VRPFASLRRG KIVVDAILSN PTVLVAQKKD FTWLGIPLSD TTLPSHLSSE
EGIDFRTKTR RVSREEAGIR WDEERDNDAR KAAEIGYIVP CKNYSQAKDN AVKHDRRFTE
IANPNSFICM DEKMHSAEQH CMDPGVEYDV KHAELEKSFG IKIPGSGLKF LSKMLKVPRK
YKFKWNSKSH KNSMSNISAK KRILERSASA ALSYFHSLSQ QKLDEPSVLS TNYDGLSLDM
LLVKGDREIS NQYDRHVPYG EQSLANDLDG KGYRVRGKRL LGVKKASTLD KFTVSCDPFL
MTVDRLCALL QTKRSPSVED IVNSSESETL SSQRGDISMN VVNQNTDDVP HGNRSGNQPR
DFTFKKHEHQ PVANHWRPSW PRNKKLKEAV FNILTGSSKK LTGRADPNAP HLSDELEKLP
AVYVEKTLPV MLDSVQFKGG TLLLLAYGDT EPREMRNVHG HVKFQNHYGR VYVQLGGNCN
MWRSDVTSED GGLLSVDVFV DTVEQNWHAN LNVANFFVPI FERILEIPIE WSKGRATGEV
HLCMSRGESF PNLHGQLDVT GLGFHINDAP SSFSDVSASL SFRGQRIFLH NANGWFGKVP
LEASGDFGIH PDEGEFHLMC QVPYVEINAL MKTFKMKPLF FPLAGSVTAV FNCQGPLDAP
VFVGSCMVSR KIAYLSPDLP TSLAYEAMLK NKEAGAVAAF DRVPFSYLSA NFTFNTDNCV
ADLYGIRATL VDGGEIRGAG NAWICPEGEV DDTALDVNFS GNISFDKVLH RYMPEYFNIG
MLKLGDLTGE TKLSGALLKP RFDIKWAAPK ADGSLTDARG DIVISHDNII VNSSSVAFDL
FTKLDTSYHD PCLSHQDFTQ GEAMPFVVEG LDLDLRMRGF EFFSLVSSYP FDSPRPTHLK
ATGRIKFLGK IKRHSTTKDG DVGSDKCEDA AAISSLDGDI SISSLKLNQL ILAPQLSGRL
SVSRDHVKLD AAGRPDESLT LDFIGPLQPN SDENVQSGKL LSFSLQKGQL RANACFQPQQ
SATLEIRNFP LDELELASLR GLIQKAEIQL NLQKRRGHGL LSVIRPKFSG VLGEALDVAV
RWSGDVCFML SGRLEVMITV EKTILEQSNS RYELQGEYVL PGSRDRDLGQ KEAGSFLMRA
MTGHLGSVIS SMGRWRMRLE VPKAEVAEML PLARLLSRST DPAVHSRSKD LFIQSVQNLC
LQAENLRDLL EEIRGYYTPP SEVVLEDLSL PGLAELKGHW HGSLDASGGG NGDTLAEFDF
HGDDWEWGTY KTQRVLATGS YNNDDGLRLK EMLIQKGNAT LHADGTLLGP KTNLHFAVLN
FPVSLIPTLV EVVESSATDI VHSLRKLLSP IKGILHMEGD LRGSLEKPEC DVQVRLLDGA
VGGIDLGRAE VFASLTSNSR FLFNSNFEPF VQNGHVHIQG SVPVSFSQKN MSEGEVSETD
RGGAVKIPSW AKEKEDDEKR TSRDRSEERW DSQLAESLKG LYWNILDAGE VRLEADIKDG
GMTLLTAISP YANWLQGNAD IRLQVGGTVD HPVLDGSASF HRASISSPVL RKPLTNFGGT
LHVKSNRLCI TSLESRVSRK GKLVVKGNLP LRSNEASAGD GIELKCEVLE VRAKNFLSCQ
VDTQLQITGS MLQPTISGNI KLSQGEAYLP HDKGGGAAPL NRLAANQYSI PGAAINQAVS
SRYFARFFGT ERASSGMKFS QSTGKSNSVE KEIEEVKMKP NMDIRLSDMK LVLGPELRIM
YPLILNFAVS GELELDGMAH PKFIKPKGVL TFENGDVNLV ATQVRLKREH LNVAKFEPEH
GLDPLLDLAL VGSEWQFRVQ SRASNWQDKL VVTSTRSVEQ DALSPSEAAK VFESQLAESI
LEGDGQLAFK KLATATLGTI MPRIEGKGEF GQARWRLVYA PQIPSLLSVD PTVDPLKSLA
SNISFGTEVE VQLGKRLQAS VVRQMKDSEM AMQWTLIYQL TSRLRVLLQS APSKRLLFEY
SATSQD
