TI441_ARATH
ID TI441_ARATH Reviewed; 474 AA.
AC Q1PF33; Q9SIL8;
DT 06-FEB-2013, integrated into UniProtKB/Swiss-Prot.
DT 16-MAY-2006, sequence version 1.
DT 03-AUG-2022, entry version 90.
DE RecName: Full=Mitochondrial import inner membrane translocase subunit TIM44-1;
DE Flags: Precursor;
GN Name=TIM44-1; OrderedLocusNames=At2g20510; ORFNames=T13C7.10;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=17147637; DOI=10.1111/j.1467-7652.2006.00183.x;
RA Underwood B.A., Vanderhaeghen R., Whitford R., Town C.D., Hilson P.;
RT "Simultaneous high-throughput recombinational cloning of open reading
RT frames in closed and open configurations.";
RL Plant Biotechnol. J. 4:317-324(2006).
RN [4]
RP IDENTIFICATION.
RX PubMed=12692332; DOI=10.1104/pp.102.016808;
RA Murcha M.W., Lister R., Ho A.Y., Whelan J.;
RT "Identification, expression, and import of components 17 and 23 of the
RT inner mitochondrial membrane translocase from Arabidopsis.";
RL Plant Physiol. 131:1737-1747(2003).
RN [5]
RP TISSUE SPECIFICITY.
RX PubMed=14730085; DOI=10.1104/pp.103.033910;
RA Lister R., Chew O., Lee M.N., Heazlewood J.L., Clifton R., Parker K.L.,
RA Millar A.H., Whelan J.;
RT "A transcriptomic and proteomic characterization of the Arabidopsis
RT mitochondrial protein import apparatus and its response to mitochondrial
RT dysfunction.";
RL Plant Physiol. 134:777-789(2004).
CC -!- FUNCTION: Essential component of the PAM complex, a complex required
CC for the translocation of transit peptide-containing proteins from the
CC inner membrane into the mitochondrial matrix in an ATP-dependent
CC manner. Recruits mitochondrial HSP70 to drive protein translocation
CC into the matrix using ATP as an energy source (By similarity).
CC {ECO:0000250}.
CC -!- SUBUNIT: Probable component of the PAM complex at least composed of a
CC mitochondrial HSP70 protein, TIMM44 and TIMM14. The complex interacts
CC with the TIMM23 component of the TIM17:23 complex (By similarity).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q1PF33-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q1PF33-2; Sequence=VSP_044958;
CC -!- TISSUE SPECIFICITY: Expressed in roots, flowers, young cotyledons and
CC leaves. {ECO:0000269|PubMed:14730085}.
CC -!- SIMILARITY: Belongs to the Tim44 family. {ECO:0000305}.
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DR EMBL; AC007109; AAD25651.2; -; Genomic_DNA.
DR EMBL; CP002685; AEC07020.1; -; Genomic_DNA.
DR EMBL; CP002685; ANM63221.1; -; Genomic_DNA.
DR EMBL; DQ446533; ABE65836.1; -; mRNA.
DR PIR; B84590; B84590.
DR RefSeq; NP_001325325.1; NM_001335691.1. [Q1PF33-1]
DR RefSeq; NP_565473.1; NM_127610.1. [Q1PF33-2]
DR AlphaFoldDB; Q1PF33; -.
DR SMR; Q1PF33; -.
DR STRING; 3702.AT2G20510.1; -.
DR PaxDb; Q1PF33; -.
DR PRIDE; Q1PF33; -.
DR EnsemblPlants; AT2G20510.1; AT2G20510.1; AT2G20510. [Q1PF33-2]
DR EnsemblPlants; AT2G20510.3; AT2G20510.3; AT2G20510. [Q1PF33-1]
DR GeneID; 816572; -.
DR Gramene; AT2G20510.1; AT2G20510.1; AT2G20510. [Q1PF33-2]
DR Gramene; AT2G20510.3; AT2G20510.3; AT2G20510. [Q1PF33-1]
DR KEGG; ath:AT2G20510; -.
DR Araport; AT2G20510; -.
DR TAIR; locus:2054759; AT2G20510.
DR eggNOG; KOG2580; Eukaryota.
DR HOGENOM; CLU_038444_0_0_1; -.
DR InParanoid; Q1PF33; -.
DR OMA; ISCKEIR; -.
DR PRO; PR:Q1PF33; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q1PF33; baseline and differential.
DR Genevisible; Q1PF33; AT.
DR GO; GO:0005743; C:mitochondrial inner membrane; IBA:GO_Central.
DR GO; GO:0005744; C:TIM23 mitochondrial import inner membrane translocase complex; TAS:TAIR.
DR GO; GO:0015462; F:ABC-type protein transporter activity; TAS:TAIR.
DR GO; GO:0051087; F:chaperone binding; IBA:GO_Central.
DR GO; GO:0030150; P:protein import into mitochondrial matrix; IBA:GO_Central.
DR GO; GO:0006626; P:protein targeting to mitochondrion; TAS:TAIR.
DR InterPro; IPR032710; NTF2-like_dom_sf.
DR InterPro; IPR039544; Tim44-like.
DR InterPro; IPR007379; Tim44-like_dom.
DR PANTHER; PTHR10721; PTHR10721; 1.
DR Pfam; PF04280; Tim44; 1.
DR SMART; SM00978; Tim44; 1.
DR SUPFAM; SSF54427; SSF54427; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Coiled coil; Membrane; Mitochondrion;
KW Mitochondrion inner membrane; Reference proteome; Transit peptide.
FT TRANSIT 1..54
FT /note="Mitochondrion"
FT /evidence="ECO:0000255"
FT CHAIN 55..474
FT /note="Mitochondrial import inner membrane translocase
FT subunit TIM44-1"
FT /id="PRO_0000420941"
FT REGION 132..165
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 68..88
FT /evidence="ECO:0000255"
FT COMPBIAS 142..165
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 26..27
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_044958"
SQ SEQUENCE 474 AA; 54299 MW; 1B1B8DA89464A362 CRC64;
MAIRKIIRDL LITKQPLLRQ LFHQRVLRAN ARSEFLPAIG YTSHRRFSVF TEFSKNIRGE
AHSNPEFERT VKELKERTEE FKGVTEDLKV RTKQTTEKLY KQADGVWTEA ESAAKKVSSS
VKDKLSAASE EVKESFKLGK EENAESASSS GTRASQGEKQ QSGSTEELHT FFAKFKSSLS
SPKVSEVFYR LKEAKPFDIV KQALDIVKDE LRGNPSRKKF LEHTPPPPFT GERSMRTEMV
VTQTKQSKLQ QKWESFREKM QGSPVFKRLS GMSEPVVNKS QEIAEDVREI WETSDNPIVH
KIQDMNEKFL KETDSASTYK EIRSRDPSFS LPDFAAEIEE VIKPVLNAYS EGDVETLKKY
CSKEVIERCT AERTAYQTHG VLFDNKLLHI SEVSVSVTKM MGDSPIIIAK FQTQEIYCVR
DENGEIQEGG QDTIHTVYHE WAMQQVETTE LGEDAIYPIW RLREMCRNGV QALI
