ID   TI442_ARATH             Reviewed;         469 AA.
AC   Q5XF06; Q8S8E5;
DT   06-FEB-2013, integrated into UniProtKB/Swiss-Prot.
DT   23-NOV-2004, sequence version 1.
DT   25-MAY-2022, entry version 109.
DE   RecName: Full=Mitochondrial import inner membrane translocase subunit TIM44-2;
DE   Flags: Precursor;
GN   Name=TIM44-2; OrderedLocusNames=At2g36070; ORFNames=F11F19.2;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10617197; DOI=10.1038/45471;
RA   Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA   Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA   Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA   Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA   Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA   Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA   Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT   "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL   Nature 402:761-768(1999).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA   Cheuk R., Chen H., Kim C.J., Shinn P., Ecker J.R.;
RT   "Arabidopsis ORF clones.";
RL   Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   IDENTIFICATION.
RX   PubMed=12692332; DOI=10.1104/pp.102.016808;
RA   Murcha M.W., Lister R., Ho A.Y., Whelan J.;
RT   "Identification, expression, and import of components 17 and 23 of the
RT   inner mitochondrial membrane translocase from Arabidopsis.";
RL   Plant Physiol. 131:1737-1747(2003).
RN   [5]
RP   TISSUE SPECIFICITY, SUBCELLULAR LOCATION, IDENTIFICATION BY MASS
RP   SPECTROMETRY, AND INDUCTION.
RX   PubMed=14730085; DOI=10.1104/pp.103.033910;
RA   Lister R., Chew O., Lee M.N., Heazlewood J.L., Clifton R., Parker K.L.,
RA   Millar A.H., Whelan J.;
RT   "A transcriptomic and proteomic characterization of the Arabidopsis
RT   mitochondrial protein import apparatus and its response to mitochondrial
RT   dysfunction.";
RL   Plant Physiol. 134:777-789(2004).
CC   -!- FUNCTION: Essential component of the PAM complex, a complex required
CC       for the translocation of transit peptide-containing proteins from the
CC       inner membrane into the mitochondrial matrix in an ATP-dependent
CC       manner. Recruits mitochondrial HSP70 to drive protein translocation
CC       into the matrix using ATP as an energy source (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Probable component of the PAM complex at least composed of a
CC       mitochondrial HSP70 protein, TIMM44 and TIMM14. The complex interacts
CC       with the TIMM23 component of the TIM17:23 complex (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC       {ECO:0000269|PubMed:14730085}.
CC   -!- TISSUE SPECIFICITY: Expressed in roots, flowers, young cotyledons and
CC       leaves. {ECO:0000269|PubMed:14730085}.
CC   -!- INDUCTION: Up-regulated after antimycin A or rotenone treatments.
CC       {ECO:0000269|PubMed:14730085}.
CC   -!- SIMILARITY: Belongs to the Tim44 family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAM15344.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AC007017; AAM15344.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; CP002685; AEC09202.1; -; Genomic_DNA.
DR   EMBL; BT015810; AAU94373.1; -; mRNA.
DR   PIR; E84776; E84776.
DR   RefSeq; NP_181151.3; NM_129166.5.
DR   AlphaFoldDB; Q5XF06; -.
DR   SMR; Q5XF06; -.
DR   STRING; 3702.AT2G36070.1; -.
DR   iPTMnet; Q5XF06; -.
DR   PaxDb; Q5XF06; -.
DR   PRIDE; Q5XF06; -.
DR   ProteomicsDB; 234372; -.
DR   EnsemblPlants; AT2G36070.1; AT2G36070.1; AT2G36070.
DR   GeneID; 818180; -.
DR   Gramene; AT2G36070.1; AT2G36070.1; AT2G36070.
DR   KEGG; ath:AT2G36070; -.
DR   Araport; AT2G36070; -.
DR   TAIR; locus:2039195; AT2G36070.
DR   eggNOG; KOG2580; Eukaryota.
DR   HOGENOM; CLU_038444_0_0_1; -.
DR   InParanoid; Q5XF06; -.
DR   OMA; AKTAMHS; -.
DR   OrthoDB; 1041560at2759; -.
DR   PhylomeDB; Q5XF06; -.
DR   PRO; PR:Q5XF06; -.
DR   Proteomes; UP000006548; Chromosome 2.
DR   ExpressionAtlas; Q5XF06; baseline and differential.
DR   Genevisible; Q5XF06; AT.
DR   GO; GO:0005743; C:mitochondrial inner membrane; HDA:TAIR.
DR   GO; GO:0005739; C:mitochondrion; HDA:TAIR.
DR   GO; GO:0005744; C:TIM23 mitochondrial import inner membrane translocase complex; TAS:TAIR.
DR   GO; GO:0015462; F:ABC-type protein transporter activity; TAS:TAIR.
DR   GO; GO:0051087; F:chaperone binding; IBA:GO_Central.
DR   GO; GO:0030150; P:protein import into mitochondrial matrix; IBA:GO_Central.
DR   GO; GO:0006626; P:protein targeting to mitochondrion; TAS:TAIR.
DR   InterPro; IPR032710; NTF2-like_dom_sf.
DR   InterPro; IPR039544; Tim44-like.
DR   InterPro; IPR007379; Tim44-like_dom.
DR   PANTHER; PTHR10721; PTHR10721; 1.
DR   Pfam; PF04280; Tim44; 1.
DR   SMART; SM00978; Tim44; 1.
DR   SUPFAM; SSF54427; SSF54427; 1.
PE   1: Evidence at protein level;
KW   Coiled coil; Membrane; Mitochondrion; Mitochondrion inner membrane;
KW   Reference proteome; Transit peptide.
FT   TRANSIT         1..44
FT                   /note="Mitochondrion"
FT                   /evidence="ECO:0000255"
FT   CHAIN           45..469
FT                   /note="Mitochondrial import inner membrane translocase
FT                   subunit TIM44-2"
FT                   /id="PRO_0000420942"
FT   REGION          133..169
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          208..228
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          60..97
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        140..169
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   469 AA;  53646 MW;  5E90C81E8A1FD1DA CRC64;
     MASRKLVRDL LITKQPLLQQ LVHQRRVGAR LGLLQGNGFA SHRRFSVFSE FSKKIRGEAD
     SNPEFQKTVK EFKERAEELQ GVKEDLKVRT KQTTEKLYKQ GQGVWTEAES VAKKVSSSVK
     DKFSAATEEV KESFKLGKEE SAESASSSGT GTTEGEKQQQ QSGSTEEQDT FFGKFKSSIS
     SPKLSEAFHK PLDFAKKGLD IVKDELRGNP SKRKHLEYTP PPPFTGERST RTEMVIMPTK
     QSKWQKKWES LREKMQGYPV FKRLSGMSEP VVNKSQEIAE DVREKWETSD NPIVHKIQDM
     NERIFEETGS ASTYKEIRRR DPSFSLPDFV SEIQEAIRPV LNAYSKGDAK TLKKYCSKEL
     IERCTAEHRA FTSQGYFFDH KLLHVSEVDI QETKMMGTTP VIIVRFQTQE IFCVRDQDGK
     IKEGGQDTIH TVYYDWAMQQ VDAAELGEDA IYPIWRLREM LRAGVQALI