TI50A_DROME
ID TI50A_DROME Reviewed; 343 AA.
AC Q9V9P3; Q4QPP6;
DT 06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 131.
DE RecName: Full=Mitochondrial import inner membrane translocase subunit TIM50-A;
DE AltName: Full=Tiny tim 3;
DE Flags: Precursor;
GN Name=ttm3; ORFNames=CG6691;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Testis;
RA Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M.,
RA Park S., Wan K.H., Yu C., Celniker S.E.;
RL Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP IDENTIFICATION, AND TISSUE SPECIFICITY.
RX PubMed=17435247; DOI=10.1534/genetics.107.072074;
RA Sugiyama S., Moritoh S., Furukawa Y., Mizuno T., Lim Y.M., Tsuda L.,
RA Nishida Y.;
RT "Involvement of the mitochondrial protein translocator component tim50 in
RT growth, cell proliferation and the modulation of respiration in
RT Drosophila.";
RL Genetics 176:927-936(2007).
CC -!- FUNCTION: Essential component of the TIM23 complex, a complex that
CC mediates the translocation of transit peptide-containing proteins
CC across the mitochondrial inner membrane. {ECO:0000250}.
CC -!- SUBUNIT: Component of the TIM23 complex at least composed of Tim23,
CC Tim17 (Tim17a1, Tim17a2 or Tim17b1) and a Tim50. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane {ECO:0000250};
CC Single-pass membrane protein {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Exclusively expressed in the testis.
CC {ECO:0000269|PubMed:17435247}.
CC -!- SIMILARITY: Belongs to the TIM50 family. {ECO:0000305}.
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DR EMBL; AE014134; AAF57243.1; -; Genomic_DNA.
DR EMBL; BT023720; AAY85120.1; -; mRNA.
DR RefSeq; NP_610130.1; NM_136286.3.
DR AlphaFoldDB; Q9V9P3; -.
DR SMR; Q9V9P3; -.
DR STRING; 7227.FBpp0085289; -.
DR PaxDb; Q9V9P3; -.
DR DNASU; 35437; -.
DR EnsemblMetazoa; FBtr0085935; FBpp0085289; FBgn0032971.
DR GeneID; 35437; -.
DR KEGG; dme:Dmel_CG6691; -.
DR CTD; 35437; -.
DR FlyBase; FBgn0032971; ttm3.
DR VEuPathDB; VectorBase:FBgn0032971; -.
DR eggNOG; KOG2832; Eukaryota.
DR GeneTree; ENSGT01040000240503; -.
DR HOGENOM; CLU_048293_0_2_1; -.
DR InParanoid; Q9V9P3; -.
DR OMA; PYGYISY; -.
DR OrthoDB; 1142452at2759; -.
DR PhylomeDB; Q9V9P3; -.
DR BioGRID-ORCS; 35437; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 35437; -.
DR PRO; PR:Q9V9P3; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0032971; Expressed in testis and 8 other tissues.
DR ExpressionAtlas; Q9V9P3; baseline and differential.
DR Genevisible; Q9V9P3; DM.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005743; C:mitochondrial inner membrane; ISS:UniProtKB.
DR GO; GO:0005739; C:mitochondrion; ISS:FlyBase.
DR GO; GO:0005744; C:TIM23 mitochondrial import inner membrane translocase complex; ISS:UniProtKB.
DR GO; GO:0004722; F:protein serine/threonine phosphatase activity; ISS:UniProtKB.
DR GO; GO:0004725; F:protein tyrosine phosphatase activity; ISS:UniProtKB.
DR GO; GO:0007006; P:mitochondrial membrane organization; ISS:UniProtKB.
DR GO; GO:0007005; P:mitochondrion organization; ISS:FlyBase.
DR GO; GO:0006470; P:protein dephosphorylation; ISS:UniProtKB.
DR GO; GO:0030150; P:protein import into mitochondrial matrix; IBA:GO_Central.
DR Gene3D; 3.40.50.1000; -; 1.
DR InterPro; IPR004274; FCP1_dom.
DR InterPro; IPR036412; HAD-like_sf.
DR InterPro; IPR023214; HAD_sf.
DR InterPro; IPR027111; Tim50.
DR PANTHER; PTHR12210:SF3; PTHR12210:SF3; 1.
DR Pfam; PF03031; NIF; 1.
DR SMART; SM00577; CPDc; 1.
DR SUPFAM; SSF56784; SSF56784; 1.
DR PROSITE; PS50969; FCP1; 1.
PE 2: Evidence at transcript level;
KW Membrane; Mitochondrion; Mitochondrion inner membrane; Protein transport;
KW Reference proteome; Transit peptide; Translocation; Transmembrane;
KW Transmembrane helix; Transport.
FT TRANSIT 1..?
FT /note="Mitochondrion"
FT /evidence="ECO:0000255"
FT CHAIN ?..343
FT /note="Mitochondrial import inner membrane translocase
FT subunit TIM50-A"
FT /id="PRO_0000043121"
FT TOPO_DOM 1..57
FT /note="Mitochondrial matrix"
FT /evidence="ECO:0000255"
FT TRANSMEM 58..77
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 78..343
FT /note="Mitochondrial intermembrane"
FT /evidence="ECO:0000255"
FT DOMAIN 135..278
FT /note="FCP1 homology"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00336"
FT CONFLICT 39
FT /note="T -> S (in Ref. 3; AAY85120)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 343 AA; 40580 MW; E73D60FD597E4017 CRC64;
MHKIVWFGTL NKSIGYIGKK KTCLLSPCEK ICLNSARKTV QRCDKNYSPP KLRRIKNFYT
YSVVLGSLFS IVMWAIYKLG KPEEDHRGPI EDEFSQLPWF RQYIMRMWHT LQYYEKMMEE
PQMARLLPNV VPPPYIQPPY SLVLEIKDVL VHPDWTYQTG WRFKKRPGVD YFLQQCSRNF
EIVIYTSEQG MTAFPLLDAL DPYGYIKYRL VRGATDLVEG QHTKNLDYLN RDLSRVIVVD
CDPYTTPLHP DNSLVLTKWL GNDDDVQLFD LTAFLQLIAE HQVNDVREVL RYYRQFEDPM
EQFKDNQRRL QEQSQESIQN LPTSERQWNL TLLGRSLRGS SIK
