ID   TI8AB_MOUSE             Reviewed;          97 AA.
AC   Q4FZG7;
DT   21-MAR-2006, integrated into UniProtKB/Swiss-Prot.
DT   30-AUG-2005, sequence version 1.
DT   03-AUG-2022, entry version 112.
DE   RecName: Full=Putative mitochondrial import inner membrane translocase subunit Tim8 A-B;
GN   Name=Timm8a2; Synonyms=Timm8ab;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Oocyte;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: Putative mitochondrial intermembrane chaperone that
CC       participates in the import and insertion of some multi-pass
CC       transmembrane proteins into the mitochondrial inner membrane. Also
CC       required for the transfer of beta-barrel precursors from the TOM
CC       complex to the sorting and assembly machinery (SAM complex) of the
CC       outer membrane. Acts as a chaperone-like protein that protects the
CC       hydrophobic precursors from aggregation and guide them through the
CC       mitochondrial intermembrane space (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Heterohexamer; possibly composed of 3 copies of TIMM8AB and 3
CC       copies of TIMM13. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane {ECO:0000250};
CC       Peripheral membrane protein {ECO:0000250}; Intermembrane side
CC       {ECO:0000250}.
CC   -!- DOMAIN: The twin CX3C motif contains 4 conserved Cys residues that form
CC       2 disulfide bonds in the mitochondrial intermembrane space. However,
CC       during the transit of Timm8ab from cytoplasm into mitochondrion, the
CC       Cys residues probably coordinate zinc, thereby preventing folding and
CC       allowing its transfer across mitochondrial outer membrane (By
CC       similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the small Tim family. {ECO:0000305}.
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DR   EMBL; BC099499; AAH99499.1; -; mRNA.
DR   CCDS; CCDS37020.1; -.
DR   RefSeq; NP_001032833.1; NM_001037744.1.
DR   RefSeq; XP_006518982.1; XM_006518919.3.
DR   AlphaFoldDB; Q4FZG7; -.
DR   SMR; Q4FZG7; -.
DR   STRING; 10090.ENSMUSP00000047497; -.
DR   iPTMnet; Q4FZG7; -.
DR   PhosphoSitePlus; Q4FZG7; -.
DR   jPOST; Q4FZG7; -.
DR   MaxQB; Q4FZG7; -.
DR   PaxDb; Q4FZG7; -.
DR   PeptideAtlas; Q4FZG7; -.
DR   PRIDE; Q4FZG7; -.
DR   ProteomicsDB; 259023; -.
DR   Ensembl; ENSMUST00000045976; ENSMUSP00000047497; ENSMUSG00000071229.
DR   GeneID; 223262; -.
DR   KEGG; mmu:223262; -.
DR   UCSC; uc007var.1; mouse.
DR   CTD; 223262; -.
DR   MGI; MGI:3615492; Timm8a2.
DR   VEuPathDB; HostDB:ENSMUSG00000071229; -.
DR   eggNOG; KOG3489; Eukaryota.
DR   GeneTree; ENSGT00940000154661; -.
DR   HOGENOM; CLU_141397_1_2_1; -.
DR   InParanoid; Q4FZG7; -.
DR   OMA; KNCAERY; -.
DR   OrthoDB; 1593287at2759; -.
DR   PhylomeDB; Q4FZG7; -.
DR   TreeFam; TF106191; -.
DR   BioGRID-ORCS; 223262; 3 hits in 73 CRISPR screens.
DR   PRO; PR:Q4FZG7; -.
DR   Proteomes; UP000000589; Chromosome 14.
DR   RNAct; Q4FZG7; protein.
DR   Bgee; ENSMUSG00000071229; Expressed in animal zygote and 21 other tissues.
DR   Genevisible; Q4FZG7; MM.
DR   GO; GO:0005743; C:mitochondrial inner membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005758; C:mitochondrial intermembrane space; ISO:MGI.
DR   GO; GO:0042719; C:mitochondrial intermembrane space protein transporter complex; ISO:MGI.
DR   GO; GO:0005739; C:mitochondrion; HDA:MGI.
DR   GO; GO:0042802; F:identical protein binding; ISO:MGI.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.287.810; -; 1.
DR   InterPro; IPR004217; Tim10-like.
DR   InterPro; IPR035427; Tim10-like_dom_sf.
DR   InterPro; IPR039238; Tim8/13.
DR   PANTHER; PTHR19338; PTHR19338; 1.
DR   Pfam; PF02953; zf-Tim10_DDP; 1.
DR   SUPFAM; SSF144122; SSF144122; 1.
PE   3: Inferred from homology;
KW   Chaperone; Disulfide bond; Membrane; Metal-binding; Mitochondrion;
KW   Mitochondrion inner membrane; Protein transport; Reference proteome;
KW   Translocation; Transport; Zinc.
FT   CHAIN           1..97
FT                   /note="Putative mitochondrial import inner membrane
FT                   translocase subunit Tim8 A-B"
FT                   /id="PRO_0000228021"
FT   MOTIF           43..66
FT                   /note="Twin CX3C motif"
FT   DISULFID        43..66
FT                   /evidence="ECO:0000250"
FT   DISULFID        47..62
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   97 AA;  11283 MW;  AF9D72279CED11BB CRC64;
     MESAWSSRGT SLGSSDPQLQ RFMEAEVQKQ RVQLLIHHMT ELCWEKCMDK PGPRLDGRAE
     LCLVNCVERF IDTSQFILNR LEQTQKARPL FSERLSD