ID   BS222_STAPS             Reviewed;          50 AA.
AC   A0A0P0C3P7; C0HJT1;
DT   30-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT   20-JAN-2016, sequence version 1.
DT   25-MAY-2022, entry version 15.
DE   RecName: Full=Bacteriocin BacSp222 {ECO:0000312|EMBL:ALI97662.1};
OS   Staphylococcus pseudintermedius.
OG   Plasmid p222 {ECO:0000312|EMBL:ALI97662.1}.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus; Staphylococcus intermedius group.
OX   NCBI_TaxID=283734 {ECO:0000312|EMBL:ALI97662.1};
RN   [1] {ECO:0000312|EMBL:ALI97662.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], PROTEIN SEQUENCE, FUNCTION,
RP   BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, INDUCTION, FORMYLATION
RP   AT MET-1, AND MASS SPECTROMETRY.
RC   STRAIN=222 {ECO:0000312|EMBL:ALI97662.1};
RC   PLASMID=p222 {ECO:0000312|EMBL:ALI97662.1};
RX   PubMed=26411997; DOI=10.1038/srep14569;
RA   Wladyka B., Piejko M., Bzowska M., Pieta P., Krzysik M., Mazurek L.,
RA   Guevara-Lora I., Bukowski M., Sabat A.J., Friedrich A.W., Bonar E.,
RA   Miedzobrodzki J., Dubin A., Mak P.;
RT   "A peptide factor secreted by Staphylococcus pseudintermedius exhibits
RT   properties of both bacteriocins and virulence factors.";
RL   Sci. Rep. 5:14569-14569(2015).
CC   -!- FUNCTION: Has bacteriolytic activity against Gram-positive bacteria
CC       B.subtilis, L.lactis and M.luteus and several species from genus
CC       Staphylococcus including methicillin-resistant S.aureus, with MIC
CC       values ranging from 0.11 uM to 7.8 uM. Has no activity against Gram-
CC       negative bacteria or fungi. In vitro, has a dose-dependent cytolytic
CC       effect on eukaryotic cells. {ECO:0000269|PubMed:26411997}.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Temperature dependence:
CC         Thermostable. Retains virtually all of its activity after incubation
CC         at 100 degress Celsius for 3 hours. {ECO:0000269|PubMed:26411997};
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:26411997}.
CC   -!- INDUCTION: Mainly expressed during the logarithmic phase.
CC       {ECO:0000269|PubMed:26411997}.
CC   -!- MASS SPECTROMETRY: Mass=5921.92; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:26411997};
CC   -!- MISCELLANEOUS: Highly resistant to proteases.
CC       {ECO:0000269|PubMed:26411997}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; CP011490; ALI97662.1; -; Genomic_DNA.
DR   PDB; 5LWC; NMR; -; A=1-50.
DR   PDB; 7NYI; NMR; -; A=1-50.
DR   PDBsum; 5LWC; -.
DR   PDBsum; 7NYI; -.
DR   AlphaFoldDB; A0A0P0C3P7; -.
DR   SMR; A0A0P0C3P7; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0019835; P:cytolysis; IEA:UniProtKB-KW.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   3D-structure; Antibiotic; Antimicrobial; Bacteriocin; Cytolysis;
KW   Direct protein sequencing; Formylation; Plasmid; Secreted.
FT   PEPTIDE         1..50
FT                   /note="Bacteriocin BacSp222"
FT                   /evidence="ECO:0000269|PubMed:26411997"
FT                   /id="PRO_0000438329"
FT   MOD_RES         1
FT                   /note="N-formylmethionine"
FT                   /evidence="ECO:0000269|PubMed:26411997"
FT   HELIX           2..11
FT                   /evidence="ECO:0007829|PDB:5LWC"
FT   HELIX           13..21
FT                   /evidence="ECO:0007829|PDB:5LWC"
FT   HELIX           23..32
FT                   /evidence="ECO:0007829|PDB:5LWC"
FT   HELIX           36..49
FT                   /evidence="ECO:0007829|PDB:5LWC"
SQ   SEQUENCE   50 AA;  5893 MW;  ECAE12A48CF22114 CRC64;
     MAGLLRFLLS KGRALYNWAK SHVGKVWEWL KSGATYEQIK EWIENALGWR